Fatal intracerebral hemorrhage following thrombolytic therapy of embolic myocardial infarction in unsuspected infective endocarditis

Cerebral hemorrhage occurs in 0.2% of patients under the age of 60 years treated with thrombolytic therapy for acute myocardial infarction. A case of fatal cerebral hemorrhage following TPA therapy for myocardial infarction due to probable coronary artery embolism during unsuspected native valve infective endocarditis is reported.     

Preparation of cell bodies from the developing cerebellum: structural and metabolic integrity of the isolated cells

A method is described for the isolation from the developing rat cerebellum of cell bodies which display a high level of ultrastructural organization. The procedure, which utilizes isotonic conditions throughout, begins with a brief trypsinisation at low enzyme concentration (0.025%). Proteolysis is terminated by trypsin inhibitor and followed by short exposure to EDTA. The technique is effective with cerebella from rats up to 2 weeks after birth. Recoveries of cell bodies vary from 130-410 million/g wet weight of tissue, depending on age: this represents, in terms of recovered DNA, a mean value for yield of 33%. Suspensions contain little debris, free nuclei are rare and about 80% of the perikarya excludes trypan blue. Survey electron micrographs show that most cell bodies possess uninterrupted plasma membrane profiles and retain highly organised cytoplasmic and nuclear ultrastructure. Structural preservation is highlighted in the case of Purkinje cell bodies in which may characteristic features survive including, most notably, perisomatic spines. Metabolic integrity appears to parallel morphological preservation as judged by several functional criteria, including the ability to metabolise glucose, accumulate K+ ions and synthesize proteins. SDS-polyacrylamide gel electrophoresis shows that the tissue dossociation technique does not lead to major deletions of cell proteins and that the pattern of perikaryal protein synthesis in vitro closely resembles that in vivo. These perikaryal preparations therefore hold out great promise as a simplified system for metabolic studies and as a starting material for the derivation of purified sub-populations of cell bodies from developing cerebellum.     

Cardiopulmonary changes during flexible fiberoptic bronchoscopy

Whole-of-life tests of the C-nitroso compounds p-nitrosodimethylaniline (NDMA) and p-nitroso-diethylaniline (NDEA) have been completed in male rats and mice fed maximum tolerated doses continuously over the first halves of their respective natural lifespans. The chemicals were offered at a concentration of 300 mg/litre drinking fluid, but the doses of NDEA consumed were only 75% of the NDMA doses, in both species. Possibly because of this the results with NDEA were statistically not clear-cut, but there was a significant increase in tumour incidence after NDMA treatment in both species. The main sites of tumorigenesis after NDMA were lung, kidney and malignant lymphoma in the rats, and lung, duodenum and malignant lymphoma in the mice. The results confirm our own earlier experiment and provide the first evidence of oncogenic activity in this class of compounds.     

Venous hypertension associated with a posterior fossa dural arteriovenous fistula: another cause of bithalamic lesions on MR images

We report a case of a posterior fossa arteriovenous fistula (AVF) with bithalamic hyperintensity of MR images. The thalamic abnormality improved after surgery, suggesting reversible venous hypertension as the pathogenesis of the finding, as opposed to infarction. This manifestation of a posterior fossa AVF should be considered in the differential diagnosis of bilateral thalamic disease.     

Effect of condensing cover material on yield of an active solar still: an experimental validation

An attempt has been made to evaluate inner and outer glass temperature and its effects on yield. Numerical computations have been performed for a typical day in the month of December, 2005, for the climatic condition of New Delhi (latitude: 28°35´ N; longitude: 77°12´ E and an altitude of 216 m above mean sea level). Higher yield was observed for an active solar distillation system as compared to the passive mode due to higher operating temperature differences between water and inner glass cover. The parametric study has also been performed to find out the effects of various parameters, namely thickness of condensing cover, collector absorbing surface, wind velocity and water depth of the still. It is observed that there is significant effect on daily yield due to change in the values of collector absorbing surface, wind velocity and water depth. For all the cases, the correlation of coefficients (r) between predicted and experimental values have been verified and they showed fair agreement with 0.90 < r < 0.99 and root mean square percent deviation 3.22% < e < 22.64%. Effect of condensing cover materials, namely copper and polyvinyl chloride (PVC), on daily yield have also been investigated and compared.