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Male genital tract obstructions may result from infections, previous inguinal and scrotal surgery (vasectomy) and congenital bilateral absence of the vas deferens (CBAVD). Microsurgery can sometimes be successful in treating the obstruction. In other cases and in cases of failed surgical intervention, the patient can be treated by microsurgical or percutaneous epididymal sperm aspiration (MESA, PESA) or testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). We present the results of 39 ICSI procedures for obstructive azoospermia in 24 couples. The aetiology of the obstruction was failed microsurgery in 11 patients, CBAVD in nine and genital infections in four. Sperm retrieval was accomplished via MESA in four cases, PESA in 18 cases and via TESE in 11 cases. TESE was only applied when PESA failed to produce enough spermatozoa for simultaneous ICSI. In six patients, the ICSI procedure was performed with cryopreserved spermatozoa after an initial PESA procedure. Fertilization occurred in 47% of the metaphase II oocytes; embryo transfer was performed in 92% of procedures and resulted in a clinical pregnancy in 13/39 procedures. Ongoing pregnancy was achieved in 10/39 procedures. One pregnancy was terminated early after prenatal investigation showed a cytogenetic abnormality (47,XX+18, Edwards syndrome). The other nine pregnancies resulted in the live birth of 10 children, without any congenital abnormalities. Epididymal and testicular retrieved spermatozoa were successfully used for ICSI to treat obstructive azoospermia, and resulted in an ongoing pregnancy in 10 of 24 couples (41.6%) after 39 ICSI procedures, a success rate of 25.6% per treatment cycle and of 27.7% per embryo transfer. 相似文献
104.
JK Tong CA Hassig GR Schnitzler RE Kingston SL Schreiber 《Canadian Metallurgical Quarterly》1998,395(6705):917-921
105.
C. J. Barclay S. W. Campbell A. M. Galloway N. A. McPherson 《The International Journal of Advanced Manufacturing Technology》2013,65(1-4):127-140
Field activated sintering is receiving a growing attention as an alternative to classical sintering for its unique ability to realize high level of densification at low temperatures and using short processing times. This technique, however, is characterized by the presence of complex temperature and stress distributions and, as a result, microstructural non-uniformities may be produced in the samples. These temperature non-homogeneities depend on a large number of experimental parameters, such as die geometry, heating rate, material properties, sintering temperature, etc. In this paper, the various experimental parameters are systematically analyzed in order to evidence their role in determining the temperature distribution. Methods to control or remove temperature inhomogeneities will be discussed, particularly in the case of large samples. 相似文献
106.
The influence of polymer surface-protein binding affinity on protein ion signals in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is examined. The surfaces of poly(vinylidene fluoride) and poly(ethylene terephthalate) polymer substrates are modified by pulsed rf plasma deposition of allylamine. By varying the on/off duty cycle of the pulsed rf plasma, the polymer substrate surfaces are coated with thin films having varying densities of surface amine groups. The varying surface amine density is shown to lead to systematic changes in the surface binding affinity for the 125I-radiolabeled peptides angiotensin I and porcine insulin. Unlabeled angiotensin I and porcine insulin are then deposited on the pulsed rf plasma-modified substrates and analyzed by MALDI mass spectrometry. The experimental approach involves applying the peptide to the modified polymer surface in an aqueous phosphate-buffered saline solution and allowing the peptide solution to dry completely under ambient conditions. Subsequently, the MALDI matrix alpha-cyano-4-hydroxycinnamic acid in methanol and 10% trifluoroacetic acid in water are added to the peptide-coated modified polymer surfaces. The results of these studies demonstrate that, for the sample preparation method employed, increases in the surface peptide binding affinity lead to decreases in the peptide MALDI ion signal. 相似文献
107.
A limited number of empirical studies conducted between 1950 and 1970 used longitudinal designs to examine the relations between psychological characteristics of students and subsequent high school dropout. Five prospective studies demonstrated that modest but significant relations existed between completing high school and measures of personality gathered in the seventh, eighth or ninth grades. Stronger relations were found in three early studies that examined the link between sociometric measures gathered in late elementary school and high school graduation 6 or 7 years later. Results clarified some methodological requirements for useful research on school completion and suggested that social and emotional variables explain some of the variance in school dropout that is independent of academic performance. Implications are drawn for effective school dropout research, policies, and practices at the local, state, and national levels. (PsycINFO Database Record (c) 2011 APA, all rights reserved) 相似文献
108.
SG Willis KS Smith VL Dunn LA Gapter KH Riviere GR Riviere 《Canadian Metallurgical Quarterly》1999,37(3):867-869
Species-specific nested PCR was used to detect Treponema amylovorum, Treponema denticola, Treponema maltophilum, Treponema medium, Treponema pectinovorum, Treponema socranskii, and Treponema vincentii in dental plaque. Subjects with periodontitis harbored all species, but T. pectinovorum and T. vincentii were not found in plaque from disease-free subjects. 相似文献
109.
FJ Dilworth GR Williams AM Kissmeyer JL Nielsen E Binderup MJ Calverley HL Makin G Jones 《Canadian Metallurgical Quarterly》1997,138(12):5485-5496
110.
GR Hill JM Crawford KR Cooke YS Brinson L Pan JL Ferrara 《Canadian Metallurgical Quarterly》1997,90(8):3204-3213
The influence of bone marrow transplantation (BMT) conditioning regimens on the incidence and severity of graft-versus-host disease (GVHD) has been suggested in clinical BMT. Using murine BMT models, we show here an increase in GVHD severity in several donor-recipient strain combinations after intensification of the conditioning regimen by increasing the total body irradiation (TBI) dose from 900 cGy to 1,300 cGy. Increased GVHD was mediated by systemic increases in tumor necrosis factor alpha (TNF alpha). Histologic analysis of gastrointestinal tracts showed synergistic damage by increased TBI and allogeneic donor cells that permitted increased translocation of lipopolysacharide (LPS) into the systemic circulation. In vitro, LPS triggered excess TNF alpha from macrophages primed by the GVH reaction. In addition, macrophages isolated within 4 hours of conditioning were primed in proportion to the TBI dose itself to secrete TNF alpha. Thus, the higher TBI dose increased macrophage priming and increased gut damage after allogeneic BMT, causing higher systemic levels of inflammatory cytokines and subsequent severe GVHD. These data highlight the importance of conditioning in GVHD pathophysiology and suggest that interventions to prevent LPS stimulation of primed macrophages may limit the severity of GVHD after intensive conditioning for allogeneic BMT. 相似文献