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We examined spatial-temporal patterns of neural activity, as inferred from 700 nm light reflectance, from the dorsal hippocampus and surrounding neocortex in seven freely behaving cats following 1.5, 2.5, 3.5 and 5.0 mg/kg intravenous cocaine administration. Images were acquired using a new technique which gathered reflected light from cortical and subcortical structures. Cardiac and respiratory patterning, collected simultaneously with optical images, revealed increased rates and diminished variation after intravenous cocaine administration. Cocaine increased reflectance correlates of hippocampal neural activity in a dose-dependent fashion over a 120 min period, with a lengthening time-to-peak effect (22-76 min). The largest dose resulted in an initial decrease, followed by the greatest enhancement in neuronal activity. Correlates of neural activation in the neocortex displayed an inverse dose-response curve to that found in the hippocampus; the time-to-peak effect was shorter (6-43 min) and the maximal change was reduced. Regional patches and bands of activation occurred during the period of the cocaine response, and were more pronounced in the hippocampus than the neocortex. Procaine, administered in a similar dose, slightly increased neural activity for 10 min in both the hippocampus and neocortex, and elicited a small increase in respiration. Cocaine induces a pronounced enhancement of neural activation in the neocortex and dorsal hippocampus; the time course of activation in the hippocampus parallels an increased respiratory pattern and outlasts the neocortical response. We speculate that hippocampal activation may be related to the profound respiratory acceleration found in response to cocaine. 相似文献
75.
JD Edinger AI Fins RJ Sullivan GR Marsh DS Dailey TV Hope M Young E Shaw D Carlson D Vasilas 《Canadian Metallurgical Quarterly》1997,20(12):1127-1134
Complaints of daytime dysfunction are common among chronic insomniacs, but laboratory comparisons of insomniacs and age-matched and gender-matched normal controls have generally failed to document these complaints. However, a few studies, which allowed subjects to sleep in their homes on the nights before daytime testing, have shown some relative diurnal deficits among insomniacs. The current study compared the effects of nocturnal laboratory and home polysomnogram (PSG) studies on subsequent daytime test results among older insomniacs and normal sleepers. Insomniacs (n = 32) and normal sleepers (n = 32) were randomly assigned to first undergo three nights of nocturnal PSG monitoring either in the sleep laboratory (16 insomniacs, 16 normal sleepers) or in their homes (16 insomniacs, 16 normal sleepers). Following the third night of PSG monitoring, subjects spent 1 day in the sleep laboratory, where they completed a four-trial multiple sleep latency test along with four trials of a computer-administered performance test battery. Results showed that insomniacs, as a group, were slightly, albeit consistently, sleepier than were normal sleepers following nights of home sleep monitoring, but a reverse of this trend was found among subjects who underwent nocturnal laboratory PSG before daytime testing. Furthermore, normal sleepers showed faster reaction times on a signal detection task than did insomniacs within the subgroup who underwent home PSGs prior to such testing. However, within the subgroup that underwent nocturnal laboratory PSGs, insomniacs' signal detection reaction times were significantly faster than those shown by normal sleepers. Results provide some support for the speculation that the nocturnal PSG monitoring site, used as a precursor to daytime testing, may systematically affect daytime comparisons between insomniacs and matched controls. Moreover, these results suggest that the use of home-based nocturnal PSG monitoring prior to daytime testing may provide an enhanced understanding of insomniacs' diurnal complaints. 相似文献
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GR Douglas J Jiao JD Gingerich LM Soper JA Gossen 《Canadian Metallurgical Quarterly》1996,28(4):317-324
In order to help establish criteria for optimizing protocols for in vivo mutation studies, lacZ transgenic mice (Muta mouse) were treated with five consecutive daily doses of ethylnitrosourea (50 mg/kg), sampled at times up to 55 days after treatment, and mutant frequencies and DNA sequences determined for liver and bone marrow. In the bone marrow, the mutant frequency rose very rapidly in the first 5 days after treatment to 34 times the control frequency. Subsequently, there was a brood peak where the mutant frequency did not vary significantly, although it did appear to begin to decline after 45 days. In contrast, in the liver, the peak mutant frequency (11 times the control frequency) was not achieved until 35 days, after which there appeared to be a slow decline up to 55 days, which was not statistically significant. Once the maximum mutant frequency was reached, the mutation spectra in the two tissues were indistinguishable. In contrast to the G:C-->A:T transitions in 5'-CpG sites characteristic of untreated mice, A:T-->T:A transversions and A:T-->G:C transitions were prominent in both liver and bone marrow of ENU-treated mice, suggesting the involvement of unrepaired O2- and O4-ethylthymine adducts. In addition, G:C-->T:A transversions were induced in liver. This study demonstrates the possibility that although tissues may have different mutation fixation times, a single mutation fixation time equal to the longest time may be appropriate for in vivo mutation studies, provided that the mutation frequency does not decline appreciably after the peak is reached. This study also illustrates the necessity of ensuring that mutation characteristics are determined after optimal fixation has occurred. 相似文献
78.
GR Stewart Y Zhu W Parredes TI Tree R Guderian JE Bradley 《Canadian Metallurgical Quarterly》1997,65(1):164-170
The concentration and affinity to concanavalin A (ConA) of plasma alpha 1-acid glycoprotein (AGP) and AGP levels in extracts of liver tissues were investigated in specific-pathogen-free chickens infected with the highly virulent HPS-2 strain or virulent reference GBF-1 strain of infectious bursal disease virus. The plasma AGP value in uninfected control 3- to 7-wk-old chickens was 161.8 +/- 25.8 micrograms/ml (mean +/- SD), and ConA-unreactive and ConA-reactive AGP comprised 47% and 63% of total AGP. Increases in AGP levels in plasma and liver extracts were observed in both the HPS-2 and the GBF-1 groups, although albumin levels decreased. The plasma AGP values were higher in the HPS-2 group than in the GBF-1 group through the experimental period. In the HPS-2 group, ConA-reactive AGP reached levels 6.8-fold greater than control values and comprised 80% of total AGP at 4 days post-inoculation. In the lipopolysaccharide group, a great increase in ConA-reactive AGP was observed. 相似文献
79.
G R Wiggans 《Journal of dairy science》1986,69(11):2935-2940
Factors to estimate daily yield from one or two sampled milkings of cows milked three times a day were derived with data for 21 mo from seven Pennsylvania herds and data for 1 yr from five Utah herds. Accuracy of estimation increased with number of milkings weighed; standard deviation of error was nearly halved when information was included from two milkings instead of one. Fat yield did not increase as much as milk yield with increased time between milkings (interval); therefore, fat percentage factors increased with interval. If fewer milkings were sampled than weighed, fat yield was estimated by adjusting sample-day fat percentage. Effect of interval on protein yield was nearly the same as on milk yield. Therefore, milk factors are recommended for protein, and no adjustment to protein percentage is necessary if fewer milkings are sampled than weighed. Factors derived for milk were tested on records from 53 California and Oregon herds. Standard deviations of errors were similar to those from Utah and Pennsylvania data. 相似文献
80.
Extension factors for annualized lactation yields [365 (total lactation yield)/-days between calvings] were computed by generating 747,904 partial lactations from complete lactation records of 105,379 Israeli-Holstein cows with one to three lactations. Factors included in the model were last test yield, days pregnant, days in milk, farm type, calving season, and days remaining in the lactation. Both linear and quadratic effects were assumed for the first three factors. Primiparous and multiparous cows were analyzed separately for milk, fat, and economically fat-corrected milk [.67 (milk) + 10 (fat)] yields. Adjusted coefficients of determination computed for this model were .04 higher for fat and .01 higher for milk and economically fat-corrected milk than for an alternate model in which remaining yield was predicted as a linear function of last test yield and remaining days in milk. Correlations between actual and predicted lactations for partial lactations of less than 4 mo in milk computed by the first model were .03 higher for fat but were nearly identical to those computed by the second model for milk. Although last test yields was the most important factor in predicting annualized yields, inclusion of other factors increased accuracy of annualized fat yield prediction. 相似文献