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81.
The use of multiple freeze (-20 degrees C)-thaw cycles in combination with isoeugenol and polysorbate 80 was investigated as a method for the reduction of numbers of Listeria monocytogenes cells in a bacteriological medium. Three freeze (1 h, -20 degrees C)-thaw cycles in the presence of isoeugenol at concentrations of 0, 100, and 300 ppm resulted in average L. monocytogenes reductions of 0.69, 2.65, and 3.3 log10 MPN (most probable number) per ml, respectively. Increasing the number of freeze-thaw cycles further decreased cell numbers, with reductions of nearly 5 log10 MPN/ml being obtained with six freeze-thaw cycles. Freeze-thaw cycles were effective in reducing cell numbers at isoeugenol concentrations down to 25 ppm. Rapid freezing rates with liquid nitrogen were found to be less effective in reducing numbers of L. monocytogenes cells. Two rapid freeze-thaw cycles in the presence of 100 ppm isoeugenol and polysorbate 80 resulted in a reduction of 1.45 log10 MPN/ml. Two freezing (-20 degrees C) cycles involving slow freezing and thawing rates with samples being held frozen for 6 h for each cycle resulted in reductions larger than those obtained with faster freezing rates. It was found that complete thawing in freeze-thaw cycles was not necessary to achieve bactericidal action. The application of multiple freeze-thaw cycles in combination with low concentrations of isoeugenol could effectively reduce numbers of L. monocytogenes cells in bacteriological media. 相似文献
82.
The alternating projections algorithm (APA) is a program that is based on alternating projections and is designed to determine the three dimensional structure of proteins using distance geometry. The sampling and convergence properties of APA, as tested on bovine pancreatic trypsin inhibitor (BPTI) were previously reported in this journal. The results confirmed the program's earlier success with poly-L-alanine. However, the asymptotic convergence of the alternating projections was slow. We show that after good convergence is first obtained via alternating projections, that a weighted quadratic penalty function then produces the final conformation with better convergence in half the time required by the asymptotic convergence of alternating projections. 相似文献
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It is shown that a Markovian queue, with bulk arrivals and departures having any probability mass functions for their batch sizes, has geometrically distributed queue length at equilibrium (when this exists) provided there is an additional special bulk arrival stream, with particular rate and batch size distribution, when the server is idle. It is shown that the time-averaged input rate of the special arrivals tends to zero as the queue becomes saturated, and a heavy-traffic limit for the queue without special arrivals is derived by martingale methods. This is shown to give the same asymptotic queue length probabilities as the geometric model. The product form is then extended to tandem networks of batch queues using the reversed compound agent theorem (RCAT). In order to obtain the product form in this case, it is required that, in addition to special arrival streams, so-called ‘partial batches’ are discarded immediately from the network when there are not enough customers in the queue to fill an entire departing batch. Somewhat surprisingly it turns out that, in heavy traffic, the product-form network does not always agree with the regulated Brownian motion (RBM) diffusion limit for the standard network without special arrivals and where partial batches are not discarded, but forwarded to the next node. Indeed, we show that the two models agree in heavy traffic if and only if the skew-symmetry condition for the RBM to have a product form is satisfied. When the condition does hold, our theoretical and numerical results thus validate the use of the product-form batch networks as moderate-traffic approximations to the analogous standard queueing network model without special arrivals and where partial batches may be forwarded to the next node instead of being lost. In the case that the condition does not hold, we obtain a new product-form stationary distribution for the associated non-RBM diffusion limit. 相似文献
84.
We consider a generic mean-field scenario, in which a sequence of population models, described by discrete-time Markov chains (DTMCs), converges to a deterministic limit in discrete time. Under the assumption that the limit has a globally attracting equilibrium, the steady states of the sequence of DTMC models converge to the point-mass distribution concentrated on this equilibrium. In this paper we provide explicit bounds in probability for the convergence of such steady states, combining the stochastic bounds on the local error with control-theoretic tools used in the stability analysis of perturbed dynamical systems to bound the global accumulation of error. We also adapt this method to compute bounds on the transient dynamics. The approach is illustrated by a wireless sensor network example. 相似文献
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Alexei Merzlikine Charles Rotter Brian Rago Julie Poe Curt Christoffersen V. Hayden Thomas 《Drug development and industrial pharmacy》2013,39(9):1082-1091
Background: Chitosan glutamate and polyacrylic acid (e.g., carbomer 974P) are known to modulate the tight junctions in the intestinal wall and increase permeability and blood exposure of drugs absorbed orally by the paracellular route. Aim: To assess the impact of chitosan glutamate and carbomer 974P on the absorption of paracellularly absorbed model drug, acyclovir, in vitro and in rat in vivo. Methods: The influence of chitosan glutamate and carbomer 974P (alone and in combination with EDTA–Na2) on the in vitro Caco-2 permeability and oral pharmacokinetic profile in the rat of acyclovir was investigated. Results: In the presence of chitosan glutamate, the apparent permeability of acyclovir across Caco2 monolayer increased 4.1 times relative to control. This increase was accompanied by a significant (~60%) decrease in transepithelial electrical resistance values indicating opening of the tight junctions in the cell monolayer. In rat, chitosan glutamate doubled oral bioavailability of acyclovir and tripled the amount of acyclovir excreted unchanged into urine. In contrast, the effect of carbomer 974P was not statistically significant at 5% level. Conclusions: In conclusion, chitosan glutamate (1–3%) and chitosan glutamate (1%)/EDTA–Na2 (0.01%) are effective excipients to increase permeability of acyclovir across Caco-2 cell monolayers and the oral absorption in the rat in vivo. 相似文献
88.
Calhoun Patrick S.; Sampson William S.; Bosworth Hayden B.; Feldman Michelle E.; Kirby Angela C.; Hertzberg Michael A.; Wampler Timothy P.; Tate-Williams Faye; Moore Scott D.; Beckham Jean C. 《Canadian Metallurgical Quarterly》2000,68(5):923
The present study assessed drug use and the validity of self–reports of substance use among help–seeking veterans referred to a specialty clinic for the assessment of posttraumatic stress disorder (PTSD). Patients (n?=?341) were asked to provide a urine sample for use in drug screening as part of an evaluation of PTSD. Self–reports of substance use were compared with same–day supervised urine samples for 317 patients who volunteered to participate in a drug screening. Results suggested that self–reports were generally quite valid. Only 8% of the cases involved patients not reporting substance use detected by urine screens. A total of 42% of the participants were identified as using drugs of abuse (excluding alcohol) through self–report and urine drug screens. Among participants using drugs, PTSD diagnosis was significantly associated with greater marijuana and depressant use as compared with stimulant (cocaine and amphetamines) use. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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