Investigation of in vitro bone cell adhesion and proliferation on Ti using direct current stimulation

Our objective was to establish an in vitro cell culture protocol to improve bone cell attachment and proliferation on Ti substrate using direct current stimulation. For this purpose, a custom made electrical stimulator was developed and a varying range of direct currents, from 5 to 25 μA, was used to study the current stimulation effect on bone cells cultured on conducting Ti samples in vitro. Cell–material interaction was studied for a maximum of 5 days by culturing with human fetal osteoblast cells (hFOB). The direct current was applied in every 8 h time interval and the duration of electrical stimulation was kept constant at 15 min for all cases. In vitro results showed that direct current stimulation significantly favored bone cell attachment and proliferation in comparison to nonstimulated Ti surface. Immunochemistry and confocal microscopy results confirmed that the cell adhesion was most pronounced on 25 μA direct current stimulated Ti surfaces as hFOB cells expressed higher vinculin protein with increasing amount of direct current. Furthermore, MTT assay results established that cells grew 30% higher in number under 25 μA electrical stimulation as compared to nonstimulated Ti surface after 5 days of culture period. In this work we have successfully established a simple and cost effective in vitro protocol offering easy and rapid analysis of bone cell–material interaction which can be used in promotion of bone cell attachment and growth on Ti substrate using direct current electrical stimulation in an in vitro model.     

Comparative study of the physico-chemical parameters of the coastal waters in rivers Matla and Saptamukhi: Impacts of coastal water coastal pollution

A qualitative and quantitative research has been performed on the physico-chemical parameters of the neighboring coastal waters of the rivers Matla and Saptamukhi in the Sunderban district, West Bengal. The distribution pattern of eight physico-chemical parameters, namely pH, salinity, dissolved oxygen, suspended solids, nitrogen and phosphorus concentrations (inorganic and total) has been graphed and compared for the two rivers over a time period of ten years (??90s decade). A statistical analysis has been carried out and the correlation data between these parameters has been rationalized based on both natural and man-made activities during that time. This has pointed to various causes behind coastal pollution of river waters. The changes in water quality have been related to flood impacts, storm surge, eutrophication, domestic sewage, agricultural and industrial wastes. In order to avoid coastal degradation and maintain environmental balance, it is very important to understand the impact of these parameters on coastal zones.     

Synthesis,characterization and corrosion performance evaluation of DDR membrane for H2 separation from HI decomposition reaction

An all silica DDR (deca dodecasil rhombohedral) zeolite membrane with dense, interlocked structure has been developed for separation of H₂ from HI/I₂ mixture of HI decomposition reaction. In this work, the DDR zeolite membrane was synthesized on the seeded clay-alumina substrate within 5 days. The seeds were synthesized by sonication mediated hydrothermal process within short crystallization time which enhanced the nucleation for the membrane growth. The synthesized membranes along with seed crystals were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR), Field emission scanning electron microscope (FESEM) and energy dispersive X-ray spectroscopy (EDAX). The selectivity of hydrogen with respect to CO₂ and Ar was evaluated by single gas permeation studies at room temperature. The tests for corrosion resistance were carried out upto 120 h with both support and DDR membrane at 130 °C which confirmed the stability of membrane under the harsh HI/I₂ environment.     

Cooperative caging and transport using autonomous aquatic surface vehicles

We present a study on the cooperative control of two autonomous surface vehicles performing a caging and transport mission on the water surface. The two vehicles, connected to each other by means of a floating flexible rope, are required to capture a floating target from a given location, and transport it to a designated position. We focus on the coordination and control strategy to meet these requirements, and on its implementation on two under-actuated vehicles. We describe a multi-layered control architecture which achieves the goal, followed by simulation studies and field experiments with the two vehicles caging and transporting a floating target on the surface of a lake.     

Iodine‐Mediated Copper‐Catalyzed Efficient α‐C(sp2)‐Thiomethylation of α‐Oxoketene Dithioacetals with Dimethyl Sulfoxide in One Pot

The direct α‐Csp² H functionalization and thiomethylation of α‐oxoketene dithioacetals (DTAs) has been accomplished with dimethyl sulfoxide (DMSO) in the presence of iodine and a copper(I) salt for the first time. A prerequisite is the in situ iodination of the α‐Csp² atom of dithioacetals that could offer other reaction channels. The operationally simple one‐pot protocol includes region‐defined consecutive iodination and sulfenylation of the challenging α‐Csp² H bond of dithioacetals employing cheap and readily available reagents. DMSO here plays a dual role as thiomethyl source and solvent.

     

A novel image fusion framework for night-vision navigation and surveillance

The night-vision image fusion plays a critical role in detecting targets and obstructions in low light or total darkness, which has great importance for pedestrian recognition, vehicle navigation, surveillance and monitoring applications. The central idea is to fuse low-light visible and infrared imagery into a single output. In this paper, we describe a new fusion framework for spatially registered visual and infrared images. The proposed framework utilizes the properties of fractal dimension and phase congruency in the non-subsampled contourlet transform (NSCT) domain. The proposed framework applies multiscale NSCT on visual and IR images to get low- and high-frequency bands. The varied frequency bands of the transformed images are then fused while exploiting their characteristics. Finally, the inverse NSCT is performed to get the fused image. The performance of the proposed framework is validated by extensive experiments on different scene imaginary, where the definite advantages are demonstrated subjectively and objectively.     

Shear velocity-based uncertainty quantification for rock joint shear strength

Bulletin of Engineering Geology and the Environment - The shear strength of rock joints is an important property required in order to analyze the stability of rock slopes and tunnels. However,...     

Silicone Rubber Compatibilized Syndiotactic Polystyrene and Thermotropic Liquid Crystalline Polymer (Vectra A950) Blend

Blends of syndiotactic polystyrene (s-PS), thermotropic liquid crystalline polymer (Vectra A-950), and silicone rubber with two different loading levels, have been prepared through melt processing in internal mixer at 285°C. Silicone rubber was used as a compatibilizer for this blend system. The effect of silicon rubber on crystalline, dynamic mechanical, rhelogical, thermal properties, and phase morphology of the (s-PS/TLCP) blend has been investigated in details. With the addition of compatibilizer the viscosity of the blend system increased to an order of magnitude. Dynamic mechanical analysis (DMTA) and differential scanning calorimetry (DSC) results showed that the glass transition temperature (T_g) of the blend, in presence of silicone rubber, shifted towards lower temperature region. From FTIR analysis it is evident that the ‘C=O’ stretching frequency has shifted towards lower side. SEM analysis suggested that, the TLCP domain size is reduced in ternary blend in comparison to binary blend system.     

Performance of a stand-alone renewable energy system based on energy storage as hydrogen

Electrolytic hydrogen offers a promising alternative for long-term energy storage of renewable energy (RE). A stand-alone RE system based on energy storage as hydrogen has been developed and installed at the Hydrogen Research Institute, and successfully tested for autonomous operation with developed control system and power conditioning devices. The excess energy produced, with respect to the load requirement, has been sent to the electrolyzer for hydrogen production. When energy produced from the RE sources became insufficient, with respect to the load requirement, the stored hydrogen was fed to a fuel cell to produce electricity. The RE system components have substantially different voltage-current characteristics and they are integrated through power conditioning devices on a dc bus for autonomous operation by using a developed control system. The developed control system has been successfully tested for autonomous operation and energy management of the system. The experimental results clearly indicate that a stand-alone RE system based on hydrogen production is safe and reliable.     

Texture modeling in carbon-carbon composites based on mesophase precursor matrices

A computational modeling study of texture formation in carbon-carbon composites based on carbon fibers and carbonaceous mesophase precursors is presented. The modeling predictions on texture formation and disclination structures are quantitatively validated with extensive experimental data. The number and type of disclinations displayed by the carbonaceous mesophase matrix is shown to be governed by the elasticity of the mesophase, the carbon fiber-mesophase interfacial energy, the size of the fibers, and positional arrangement of the fibers. The simulations provide new insights on the fundamental principles that govern texturing and disclination nucleation, and on how to control the structure of carbon-carbon composites through fiber concentration, fiber cross-section, and fiber-matrix interaction.