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51.
JL Taylor-Roth PV Malven DE Gerrard SE Mills AL Grant 《Canadian Metallurgical Quarterly》1998,120(3):357-363
The successful use of proteins in pharmaceutical and other commercial applications requires close examination of their relative fragility. Because of the resultant enhanced stability, proteins are often formulated in the solid state, even though dehydration tends to alter their structure. Even in the solid form, however, proteins may become inactivated due to various deleterious processes, e.g., aggregation. This review focuses on such mechanisms, with an emphasis on case studies conducted in our laboratory. Proteins which have both disulfide bonds and free thiols may aggregate via thiol-disulfide exchange, and this process may be facilitated by lyophilization-induced structural perturbations. For proteins possessing disulfides but not free thiols, aggregation also may occur when native disulfides are beta-eliminated, thus giving rise to thiol species which can catalyze disulfide scrambling. Other deleterious processes have also been uncovered, including a formaldehyde-mediated aggregation of formalinized vaccines. It is illustrated how knowledge of such deterioration pathways makes possible the rational development of stable solid protein formulations. 相似文献
52.
Design aspects applicable to seawater cooling systems of combined power and desalination plants are discussed in this paper. Preventive measures are proposed on how to assure trouble-free seawater supply for cooling and seawater distillation. Site-specific measures are illustrated with the example of the seawater supply system to the Ruwais Utilities Complex, Abu Dhabi, which consists of a combined power and desalination plant as well as a fertilizer plant. 相似文献
53.
Jinjun Xia Amanda Weaver David E. Gerrard Gang Yao 《Sensing and Instrumentation for Food Quality and Safety》2008,2(2):75-81
The objective of this study was to determine the influence of the mechanisms regulating tenderness on optical scattering properties
of beef. Optical scattering properties of four beef muscles with different amounts of connective tissue and varying sarcomere
lengths across a range of aging periods were determined. The optical scattering coefficient of beef muscles were measured
based on a diffusive fitting of spatially resolved reflectance measurements. As a reference to beef tenderness, Warner-Bratzler
shear forces were also measured in this study. Variation in muscle composition resulted in a range of optical scattering properties
across muscles. Our results implied that connective tissue and sarcomere structure have different effects on the optical scattering
properties of muscle. In addition, proteolysis of muscle proteins significantly changes the optical scattering properties.
These results provide useful information in understanding the originations of optical scattering in beef. 相似文献
54.
Wills Thomas A.; Gibbons Frederick X.; Sargent James D.; Gerrard Meg; Lee Hye-Ryeon; Dal Cin Sonya 《Canadian Metallurgical Quarterly》2010,29(5):539
Objective: To investigate whether self-control moderates the effect of media influences on tobacco and alcohol use among youth and if so how this effect occurs. Design: In Study 1, a regional sample of 10-year olds (N = 290) was interviewed in households; attention to tobacco/alcohol advertising was assessed. In Study 2, a national sample of youth ages 10–14 years (N = 6,522) was surveyed by telephone; exposure to tobacco/alcohol use in movies was assessed. Good self-control was measured in both studies. Main Outcome Measures: Willingness to use substances and affiliation with peer substance users (Study 1); involvement in smoking or drinking (Study 2). Results: In Study 1, the effect of tobacco/alcohol advertising on predisposition for substance use was lower among persons scoring higher on good self-control. In Study 2, the effect of movie smoking/alcohol exposure on adolescent tobacco/alcohol use was lower, concurrently and prospectively, among persons scoring higher on good self-control. Moderation occurred primarily through reducing the effect of movie exposure on positive smoking/alcohol expectancies and the effect of expectancies on adolescent use; some evidence for moderation of social processes was also noted. Covariates in the analyses included demographics, sensation seeking, and IQ. Conclusion: Good self-control reduces the effect of adverse media influences on adolescent tobacco and alcohol use. Findings on the processes underlying this effect may be useful for media literacy and primary prevention programs. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
55.
Dustin D. Gerrard David T. Fullwood Denise M. Halverson Stephen R. Niezgoda 《计算机、材料和连续体(英文)》2010,15(2):129-152
The effective properties of composite materials are often strongly related to the connectivity of the material components. Many structure metrics, and related homogenization theories, do not effectively account for this connectivity. In this paper, relationships between the topology, represented via homology theory, and the effective elastic response of composite plates is investigated. The study is presented in the context of popular structure metrics such as percolation theory and correlation functions. 相似文献
56.
Wills Thomas A.; Sargent James D.; Gibbons Frederick X.; Gerrard Meg; Stoolmiller Mike 《Canadian Metallurgical Quarterly》2009,23(1):23
The authors tested a theoretical model of how exposure to alcohol cues in movies predicts level of alcohol use (ever use plus ever and recent binge drinking) and alcohol-related problems. A national sample of younger adolescents was interviewed by telephone with 4 repeated assessments spaced at 8-month intervals. A structural equation modeling analysis performed for ever-drinkers at Time 3 (N = 961) indicated that, controlling for a number of covariates, movie alcohol exposure at Time 1 was related to increases in peer alcohol use and adolescent alcohol use at Time 2. Movie exposure had indirect effects to alcohol use and problems at Times 3 and 4 through these pathways, with direct effects to problems from Time 1 rebelliousness and Time 2 movie exposure also found. Prospective risk-promoting effects were also found for alcohol expectancies, peer alcohol use, and availability of alcohol in the home; protective effects were found for mother's responsiveness and for adolescent's school performance and self-control. Theoretical and practical implications are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
57.
E.J. Wynveen B.C. Bowker A.L. Grant J.W. Lamkey K.J. Fennewald L. Henson D.E. Gerrard 《Journal of food science》2001,66(6):886-891
ABSTRACT: The ability of sodium polyphosphate (P) to alter postmortem pH declines and pork quality was investigated. Hams from electrically stimulated carcasses were injected 18 min postmortem with P or sodium bicarbonate (SB). P and SB reduced ( P 0.001) pH decline and improved (P < 0.05) color. P and SB administration improved water-holding capacity as indicated by reductions ( P 0.05) in drip loss, thaw loss, and cooking loss values. P and SB also reduced ( P 0.001) shear values. These data showed delivery of P and SB was effective in altering postmortem pH declines and improving the quality of pork. 相似文献
58.
Reservoir creation results in decomposition of flooded organic matter and increased rates of mercury methylation. Methylmercury (MeHg), the most toxic form of mercury, bioaccumulates through aquatic food webs. Our objective was to quantify the transfer of MeHg from aquatic food webs into terrestrial organisms. We examined rates of MeHg bioaccumulation in an insectivorous songbird, the tree swallow, breeding near an experimentally created reservoir. We also determined the impact of flooding and MeHg bioaccumulation on the reproductive success of these birds. Mean MeHg burdens in nestling swallows from near the experimental reservoir increased from 1,210 +/- 150 ng before flooding to 2,200 +/- 102 ng after flooding. Postflood MeHg concentrations in both the body and feathers of the birds were significantly greater than preflood MeHg concentrations. Although MeHg burdens in swallows were elevated in postflood years, we found no overt toxicological affects. An increase in dipteran productivity (the primary food source of tree swallows) after reservoir creation resulted in earlier nest initiation, larger eggs, and faster growth rates of wing and bill length in nestlings raised during postflood years. 相似文献
59.
Pale, soft, and exudative (PSE) meat has been recognized for decades and causes huge economic loss to the meat industry due to its inferior quality. Although it has been well established that fast and excessive glycolysis combined with high temperature in muscle early postmortem is the cause of PSE meat, the molecular mechanisms associated with this abnormal glycolysis remain poorly defined. Our previous studies with mice and pigs suggest that AMPK regulates muscle glycolysis postmortem. To confirm further the role of AMPK in the regulation of postmortem glycolysis, we investigated the effects of intraperitoneal injection of compound C, a specific AMPK inhibitor, on AMPK activation and glycolysis in postmortem longissimus dorsi (LD) muscle of mice. Data showed that intraperitoneal injection of compound C inhibited AMPK activation in postmortem mouse LD muscle. Simultaneously, injection of compound C inhibited glycolysis and increased muscle pH corroborating of our previous observations that postmortem glycolysis is inhibited in AMPK knockout mice. This study firmly supports that AMPK regulates glycolysis in postmortem skeletal muscle and suggests that AMPK can be a target to control postmortem glycolysis, preventing incidence of PSE meat. 相似文献
60.
The objective of this study was to determine effects of light (40-80 kg) or heavy (100-130 kg) slaughter weight and halothane status (positive, nn; negative, NN; and heterozygous, Nn), on meat quality. Longissimus muscle (LM) pH at 45 min (pH(45)) post-exsanguination was 6.25, 6.03, and 5.84 (different at p < 0.01) for NN, Nn, and nn genotype, respectively. At heavier weights (100-130 kg), genotype correlated (r = -0.71) with LM pH(45), 10th costae LM (TENLM) color score (r = -0.55), TENLM Hunter L(?)-value (r = 0.47), water holding capacity (r = 0.42) and TENLM subjective firmness-wetness score (r = 0.51). Rate constants for metmyoglobin accumulation and oxymyoglobin autoxidation, indicators for fresh meat color stability, increased (p < 0.05) with decreasing pH. Color stability for NN muscle was more stable than nn muscle (p < 0.05). Electrofocusing of myoblobin revealed two bands (MW 17.10(3)) at pI 6.1 and 6.5 across genotypes. Because differences were not observed across genotypes, an observed increase (p < 0.05) in 24 hr myoglobin autoxidation rate constant (associated with increased expression of the HAL gene) are presumed dependent upon post-mortem muscle changes. These data show that changes in halothane status affect fresh pork quality and that lowered meat quality results in further color destruction due to altered chemical reactions involving myoglobin oxidation. 相似文献