Increase of trans-resveratrol in typical Sicilian wine using β-Glucosidase from various sources

β-Glucosidase (EC 3.2.1.21) (β-G) from different sources were tested to increase the trans-resveratrol in some Sicilian wines by hydrolysing resveratrol glucoside. β-G from Aspergillus niger mould was tested as a crude and purified enzyme, and compared with the same enzyme from Saccharomyces cerevisiae yeast. Specific purification served to eliminate collateral enzyme activities so that β-G could be used simply and economically. Aspergillus niger β-G produced trans-resveratrol increases of up to 75%, with no change in physico-chemical properties and bouquet, and an increase in health and nutritional properties. S. cerevisiae β-G raised free-terpenol levels, but impaired wine colour due to anthocyanase activity.     

Self-organizing maps for surveying lubrication within squeeze film dampers

Self-organizing maps (SOMs) represent a well-known neural network technique particularly suited to classification tasks. Here, it is adopted to monitor the lubricating conditions within squeeze film dampers for rotor support and was aimed, in particular, at identifying the aspect of the pressure waves within the oil film. Pressure distribution is indeed significantly influenced by a number of factors, which affect damper operation. Results from past research in the field make it possible to infer that the pattern of pressure signals taken in the oil film represents a valuable source of information as regards the lubricating conditions within the damper. Surveillance procedures in the operation of turbomachinery could benefit from prompt detection of possible, unwanted changes in the characteristics of lubrication, for example, when modeling bearing operations within model-based schemes. In this paper, SOM capabilities are first evaluated, dealing separately with theoretically simulated data. The subsequent tests adopted theoretical data as a reference for identifying experimental conditions. Further tests were carried out to map experimental data. Despite constraints consisting in the damper motion being imposed during the theoretical and experimental tests, the results confirmed the potential of the method and encourage further tests in conditions which are closer to real operation.     

Phenolic content and antioxidant capacity versus consumer acceptance of soaked and vacuum impregnated frozen nectarines

Nectarines (Prunus persica L. cv. Maria Laura) were manually selected, cut in slices and divided into four groups: fresh, untreated frozen, soaked in osmotic solution and subsequently frozen, and vacuum impregnated (VI) and subsequently frozen. This investigation was focused on evaluation of consumer acceptance with respect to treated versus untreated frozen nectarine slices. In a preliminary acceptance test of untreated frozen nectarine slices, fruits were generally rejected on the basis of a darkened appearance and “oxidized” taste. These negative attributes were probably linked to the activity of polyphenol oxidase (PPO) and depletion of phenols due to cell rupture during freeze–thaw procedures. For these reasons, in order to evaluate the tendency of fruit to oxidation, several analyses were performed: the antioxidant capacity of phenolic fraction and the o-diphenol content were estimated by spectrophotometric assays, whereas the hydroxycinnamic acid (chlorogenic and neochlorogenic acids) composition was evaluated by high performance liquid chromatography (HPLC). Phenolic content and antioxidant capacity were found to correlate well with the acceptance level of frozen nectarine slices. In this regard a higher phenolic content associated with a higher acceptance level of nectarine samples.     

Optimization of parallel variable-speed-driven centrifugal pumps operation

This paper presents an optimization framework based on dynamic programming mathematic tool suitable to be implemented in programmable logic controllers to improve the automated control of parallel pumping loops aiming at energy conservation. The paper also contributes with simple methods to derive system curve and pumping head-flow speed-dependent characteristic from ordinary field measurements.     

User profiling and virtual agents: a case study on e-commerce services

The main contribution of this work is the design of an application framework based on both conversational agents and user profiling technologies for the development of e-commerce services. User profiles are exploited by conversational agents to help customers in retrieving potentially interesting products from a catalogue. Three techniques were used for collecting data for a usability test: eye-movement tracking, questionnaire, and recording the user-system dialogue. The main outcomes of the experimental sessions are: (1) the dialogue capabilities of the agent facilitate the interaction between the user and the e-commerce site; and, (2) user profiles improve the retrieval capabilities of the agent. Finally, some limitations of the user profiling techniques adopted in the framework are discussed and a more sophisticated content-based profiling technique is proposed.     

Electroactive carbon nanotube actuators: Soft-lithographic fabrication and electro-chemical modelling

Carbon nanotube (NT) actuators were fabricated using the micromolding method. In order to prevent the nanotubes from aggregating, they were enclosed in a partially cross-linked polyvinylalcohol–polyallylamine matrix. A unimorph matrix bender in the form of a small multi-layered strip, 5 mm × 15 mm was fabricated. The resulting composite system: NT/PVA, solid polymer electrolyte and metal contact, was characterised using impedance analysis and cyclic voltammetry. Initial measurements on electro-mechanical transduction show a conversion efficiency of 0.2 μm/mV. An electrochemical model of the impedance of the system was then developed, with which the experimental results agree fairly well.     

BIC 3, the Latest Inertial Centrifugal Balance for Mass Measurement in Weightless Conditions

The paper describes BIC 3, the latest prototype of inertial balance made at INRIM (former IMGC–CNR) in view of its possible use on board the International Space Station. The main characteristic of this instrument is its ability to work both in weightless conditions and on Earth surface with metrological performances comparable to those of a laboratory-level classic balance. BIC 3, although still based on the same centrifugal method adopted in the two previous prototypes, widely differs from them as regards configuration (constant speed), main motor characteristics (here a stepping motor is used), force transducer (the integral beam of a commercially-available balance), and consequently shows metrological performances considerably improved. The main constructional features are described and the metrological characteristics resulted from on-Earth tests are reported and discussed. A test made of 175 measurements in the range 0–150 g showed an expanded uncertainty of 4.1 mg.     

Pollution of inland waters in Brazil: the case for goal-oriented initiatives

This article examines the use of output-based aid and payment for environmental services to reduce water pollution in Brazil. Two nationwide initiatives are reviewed. First, the output-based aid concept of the River Basin Clean-up Programme (PRODES) to tackle the problem of sanitation deficit in urban areas is presented. Second, the payment for environmental services scheme supported by Brazil’s National Water Agency (ANA) through the Water Producer Programme to promote water conservation on rural properties is discussed. Both programmes are evaluated not only as financing mechanisms for water conservation but also as strategies for implementing Brazil’s water policy.