Early age and hardened performance of cement pastes combining mineral additions

To asses the influence of mineral additions (MA) at early age and on hardened performance of fluid cement based pastes, an experimental program was carried out. The design of the mixtures correspond to paste compositions used in self compacting concretes of moderated strength, as those employed for architectural applications. Two types of fillers (limestone and quartzite) have been used to substitute 50 % of cement in a reference paste, with and without a high range water reducing admixture. Then, three active MA (microsilica, nanosilica and metakaolin) were combined. A physical and mechanical characterization in the hardened state showed that the inclusion of MA to a cement-filler mixture can moderately improve the hardened performance of the pastes. Air and water cured samples were tested in order to evaluate the influence of curing conditions. At early ages (24 h), in situ temperature and ultrasonic pulse velocity were monitored on samples with limestone filler, combined with the three active MA, to study the reaction process and microstructure development, respectively. The reaction degree of the samples under study during the first 24 h was related to the microstructure development. Evaporation, drying shrinkage and cracking at early age were also monitored, considering an air flow of 3 m/s on the exposed sample surface. Some relations were described linking cracking risks at early ages with the chemical and physical phenomena involved at early age microstructure evolution.     

Scheduling manufacturing systems with blocking: a Petri net approach

This paper introduces a Petri net-based approach for scheduling manufacturing systems with blocking. The modelling of the job routings and the resource and blocking constraints is carried out with the Petri net formalism due to their capability of representing dynamic, concurrent discrete-event dynamic systems. In addition Petri nets can detect deadlocks typically found in systems with blocking constraints. The scheduling task is performed with an algorithm that combines the classical A* search with an aggressive node-pruning strategy. Tests were conducted on a variety of manufacturing systems that included classical job shop, flexible job shop and flexible manufacturing scheduling problems. The optimisation criterion was makespan. The experiments show that the algorithm performed well in all types of problems both in terms of solution quality and computing times.     

Synthesis, biological activity, and ADME properties of novel S-DABOs/N-DABOs as HIV reverse transcriptase inhibitors

Previous studies aimed at exploring the SAR of C2-functionalized S-DABOs demonstrated that the substituent at this position plays a key role in the inhibition of both wild-type RT and drug-resistant enzymes, particularly the K103N mutant form. The introduction of a cyclopropyl group led us to the discovery of a potent inhibitor with picomolar activity against wild-type RT and nanomolar activity against many key mutant forms such as K103N. Despite its excellent antiviral profile, this compound suffers from a suboptimal ADME profile typical of many S-DABO analogues, but it could, however, represent a promising candidate as an anti-HIV microbicide. In the present work, a new series of S-DABO/N-DABO derivatives were synthesized to obtain additional SAR information on the C2-position and in particular to improve ADME properties while maintaining a good activity profile against HIV-1 RT. In vitro ADME properties (PAMPA permeation, water solubility, and metabolic stability) were also experimentally evaluated for the most interesting compounds to obtain a reliable indication of their plasma levels after oral administration.     

Surface enhanced fluorescence of anti-tumoral drug emodin adsorbed on silver nanoparticles and loaded on porous silicon

Fluorescence spectra of anti-tumoral drug emodin loaded on nanostructured porous silicon have been recorded. The use of colloidal nanoparticles allowed embedding of the drug without previous porous silicon functionalization and leads to the observation of an enhancement of fluorescence of the drug. Mean pore size of porous silicon matrices was 60 nm, while silver nanoparticles mean diameter was 50 nm. Atmospheric and vacuum conditions at room temperature were used to infiltrate emodin-silver nanoparticles complexes into porous silicon matrices. The drug was loaded after adsorption on metal surface, alone, and bound to bovine serum albumin. Methanol and water were used as solvents. Spectra with 1 μm spatial resolution of cross-section of porous silicon layers were recorded to observe the penetration of the drug. A maximum fluorescence enhancement factor of 24 was obtained when protein was loaded bound to albumin, and atmospheric conditions of inclusion were used. A better penetration was obtained using methanol as solvent when comparing with water. Complexes of emodin remain loaded for 30 days after preparation without an apparent degradation of the drug, although a decrease in the enhancement factor is observed. The study reported here constitutes the basis for designing a new drug delivery system with future applications in medicine and pharmacy.     

Evaluating the In Vitro Metabolism of Docosahexaenoic Acid in Sheep Rumen Fluid

Rumen metabolism (e.g., biohydrogenation) of dietary unsaturated fatty acids (FA) is one of the main reasons why ruminant fats tend to be highly saturated and contain many isomerized FA intermediates. The process by which long-chain (20- to 24-carbon FA) polyunsaturated FA (LC-PUFA) are metabolized by rumen bacteria is not as well understood as that of linoleic or linolenic acids. In order to better understand the fate of LC-PUFA in the rumen several concentrations of docosahexaenoic acid (DHA) were evaluated in in vitro batch incubations ranging from 100 to 1,500 μg per 6 mL of incubation volume using rumen fluid from sheep and incubated for 0, 1, 2, 3, and 6 h. From the results, it was shown that DHA was extensively metabolized at low (100 to 300 μg/6 mL incubation volume), but not at high level of inclusion (800 μg). At 300 μg of DHA most of the depleted DHA was recovered as LC-DHA metabolites within the first 6 h of incubation, and at the lowest levels (100 μg of incubation volume) further metabolism is apparent at 6 h. Using SP-2560 GC columns several LC-DHA metabolites were shown to elute after 24:0 and just past DHA, a region generally free of interfering FA. The present in vitro study would appear to be a useful method to evaluate the production of DHA metabolites in combination with its depletion.     

Effect of planting density on nutritional quality of green-chopped corn for silage

The objective of this on-farm study was to determine the effect of corn planting density on the nutritional quality of whole-plant corn for silage. This study was performed in a commercial 1,900-cow dairy farm located in Piedritas (Buenos Aires, Argentina). Two commercial hybrids (A and B) were planted in experimental plots within a cornfield destined for corn silage. Hybrids were sown at a theoretical seeding rate of 60,000, 70,000, 80,000, and 90,000 seeds/ha in 4 replicates per hybrid. Plots were eight 50-m-long rows separated by 52 cm. Corn was planted with a no-till seeder equipped with a pneumatic dosing machine. Ten plants within each plot were cut by hand at 15 cm above ground. Whole plants were chopped, weighed, mixed thoroughly, and frozen until analysis. Nutritional composition was determined by near-infrared reflectance spectroscopy. Harvesting occurred at one-quarter milk-line [31.4% dry matter (DM)] and one-half milk-line (34.5% DM) stages of maturity for hybrids B and A, respectively. No interactions between hybrid and planting density were observed for any of the variables of interest. Planting density did not affect either plant DM weight or DM, crude protein, neutral detergent fiber, acid detergent fiber, or starch concentrations of whole-plant corn. Dry matter yield was significantly increased at higher planting densities. The similar per-plant biomass and nutritional quality among different densities can be explained by the abundant precipitation observed during this growing season (719 mm since the beginning of fallow until harvest). In conclusion, greater yields of silage can be obtained by increasing corn planting density without affecting its nutritional composition, although the effect of planting density with limiting resources (e.g., precipitation) still needs to be elucidated.     

Code aperture optimization for spectrally agile compressive imaging

Coded aperture snapshot spectral imaging (CASSI) provides a mechanism for capturing a 3D spectral cube with a single shot 2D measurement. In many applications selective spectral imaging is sought since relevant information often lies within a subset of spectral bands. Capturing and reconstructing all the spectral bands in the observed image cube, to then throw away a large portion of this data, is inefficient. To this end, this paper extends the concept of CASSI to a system admitting multiple shot measurements, which leads not only to higher quality of reconstruction but also to spectrally selective imaging when the sequence of code aperture patterns is optimized. The aperture code optimization problem is shown to be analogous to the optimization of a constrained multichannel filter bank. The optimal code apertures allow the decomposition of the CASSI measurement into several subsets, each having information from only a few selected spectral bands. The rich theory of compressive sensing is used to effectively reconstruct the spectral bands of interest from the measurements. A number of simulations are developed to illustrate the spectral imaging characteristics attained by optimal aperture codes.     

Effects of combining microbial transglutaminase and high pressure processing treatments on the mechanical properties of heat-induced gels prepared from arrowtooth flounder (Atheresthes stomias)

The objective of this study was to evaluate the effect of setting conditions (25 °C for 2 h or 40 °C for 30 min) and combining of microbial transglutaminase (MTGase) and high pressure processing (HPP) on the mechanical properties of heat induced gels obtained from paste from arrowtooth flounder (Atheresthes stomias). Treatments included fish paste control without added MTGase, fish paste incubated with MTGase but not pressurized (MTGase + cooking), fish paste incubated with MTGase and pressurized at 600 MPa for 5 min (MTGase + HPP + cooking) and fish paste pressurized at 600 MPa for 5 min and incubated with MTGase (HPP + MTGase + cooking). The controls and the treated samples were then subjected to one of two thermal treatments: 90 °C for 15 min or 60 °C for 30 min before cooking at 90 °C for 15 min. Samples of fish paste heated at 60 °C before cooking could not be used to prepare gels for texture profile analysis (TPA). TPA showed that pressurization improved the mechanical properties of gels made from paste treated with MTGase and set at 25 °C. The opposite was observed for samples set at 40 °C. Setting at 40 °C appeared to induce proteolytic degradation of myofibrillar proteins.