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21.
22.
Use of β-hydroxyacyl-CoA-dehydrogenase (HADH) activity to differentiate frozen from unfrozen fish and shellfish 总被引:1,自引:0,他引:1
Manuela Fernández Sergio Mano Gonzalo D. García de Fernando Juan A. Ordóñez L. Hoz 《European Food Research and Technology》1999,209(3-4):205-208
Further work on an enzymic method to differentiate frozen from unfrozen fish and shellfish is reported. The method is based
on the release of the β-hydroxyacyl-CoA-dehydrogenase (HADH) from mitochondria during freezing. Enzymic activity was evaluated
in fresh and frozen thawed samples from sole (Solea solea), sea bream (Pagellus centrodontus), hake (Merluccius merluccius), gilt headed bream (Sparus aurata), sea bass (Dicentrarchus labrax), salmon (Salmo salar), prawn (Penaeus japonicus) and Norwegian lobster (Nephrops norvegicus). Changes in the HADH activity of fresh and frozen thawed samples were compared after freezing at –196 °C for 15 min. Two
values were obtained: U (by dividing: HADH activity of samples frozen at –196 °C, then thawed/HADH activity of unfrozen samples) and F (by dividing: HADH activity of samples frozen at –18 °C, thawed, then frozen at –196 °C /HADH activity of samples frozen
at –18 °C, then thawed). Statistical analysis showed significant differences (P≤0.05) between both quotients for gilt headed bream, salmon, sea bream, sole and prawn, and an arbitrary limit was set at
2 to differentiate frozen thawed from unfrozen samples. The application of this limit made it possible to discriminate the
unfrozen from the frozen thawed state of around 90% of the total samples analysed. Best results were obtained for prawn (100%
of samples differentiated). In the present paper, a laboratory routine is proposed based on the comparison of the HADH activity
of a sample analysed straight away and that of a sample frozen at –196 °C and then thawed. The reported method is simple
and fast. The entire laboratory procedure can be performed in 45 min.
Received: 20 July 1998 / Revised version: 2 November 1998 相似文献
23.
Gonzalo Ricardo Ríos-Muoz Francisco Fernndez-Avils ngel Arenal 《International journal of molecular sciences》2022,23(8)
The maintaining and initiating mechanisms of atrial fibrillation (AF) remain controversial. Deep learning is emerging as a powerful tool to better understand AF and improve its treatment, which remains suboptimal. This paper aims to provide a solution to automatically identify rotational activity drivers in endocardial electrograms (EGMs) with convolutional recurrent neural networks (CRNNs). The CRNN model was compared with two other state-of-the-art methods (SimpleCNN and attention-based time-incremental convolutional neural network (ATI-CNN)) for different input signals (unipolar EGMs, bipolar EGMs, and unipolar local activation times), sampling frequencies, and signal lengths. The proposed CRNN obtained a detection score based on the Matthews correlation coefficient of 0.680, an ATI-CNN score of 0.401, and a SimpleCNN score of 0.118, with bipolar EGMs as input signals exhibiting better overall performance. In terms of signal length and sampling frequency, no significant differences were found. The proposed architecture opens the way for new ablation strategies and driver detection methods to better understand the AF problem and its treatment. 相似文献
24.
25.
Emilia Ferrer Amparo Alegría Rosaura Farr Pedro Abelln Fernando Romero Gonzalo Clemente 《Journal of the science of food and agriculture》2003,83(5):465-472
The evolution of the Maillard reaction (MR) by measuring the available lysine and furosine (FUR) contents in adapted and follow‐up powdered milk‐based infant formulas over the shelf‐life storage period, at 20 and 37 °C, was studied. Available lysine and FUR contents were determined by fluorimetry and high‐performance liquid chromatography respectively. Statistically significant differences were found between adapted and follow‐up infant formulas with respect to the available lysine and FUR contents. Available lysine contents decreased significantly throughout the storage time, and the contents at 37 °C were lower than at 20 °C. A statistically significant increase in FUR contents was observed during the storage period, with the contents being high at 37 °C than at 20 °C. A simple regression analysis between the available lysine and FUR contents during (a) the first year (b) the second year and (c) the two storage years was applied. The best correlations were obtained during the first year of storage. The results obtained show a clear MR evolution during the storage of infant formulas. Copyright © 2003 Society of Chemical Industry 相似文献
26.
Gonzalo Paíno-Campa Mjesús Pea-egido Concepcin García-Moreno 《Journal of the science of food and agriculture》1991,56(1):85-93
The present work describes a method for the determination of free and total sulphite in fresh sausages and its implementation on 48 samples. The method includes the extraction of sulphite, both free and total, by dissolution of the sample in a suitable solvent and determination by high performance liquid chromatography with electrochemical detection. The method is that previously reported by Kim and Kim (J Food Sci 51 1986 1360–1361) improved by certain modifications the principal of which consists of working atpH 10 for the extraction of total sulphite. Use of this pH permits reversible dissociation of the combined sulphite. In reproducibility assays carried out on sausages, variation coefficients of 3.3 and 5% were obtained for free and total sulphite, respectively. To evaluate recovery, sulphite was added to the samples as hydroxymethylsulphonate. Mean recovery was 86.4% with a variation coefficient of 3.8%. In 62% of the sausages analysed, amounts of total sulphites higher than 450 μ SO2 g?1 were found. Among the samples, considerable variation was observed in the distribution of sulphite between the free and combined forms. 相似文献
27.
Robbins Michael S.; Turner Charles W.; Alexander James F.; Perez Gonzalo A. 《Canadian Metallurgical Quarterly》2003,17(4):534
This study examined the relationship between alliance and retention in family therapy. Alliance was examined at the individual (parent and adolescent) and family levels (within-family differences). Participants were 34 families who received functional family therapy for the treatment of adolescent (aged 12-18 years) behavior problems. Families were classified as treatment dropouts (n=14) or completers (n=20). Videotapes of the first sessions were rated to identify parent and adolescent alliances with the therapist. Results demonstrated that individual parent and adolescent alliances did not predict retention. However, as hypothesized, dropout cases had significantly higher unbalanced alliances (parent minus adolescent) than did completer cases. These findings highlight the importance of alliances in functional family therapy and suggest that how the alliance operates in conjoint family therapy may be a function of systemic rather than of individual processes. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
28.
Chemical composition and antioxidant properties of Laurus nobilis L. and Myrtus communis L. essential oils from Morocco and evaluation of their antimicrobial activity acting alone or in combined processes for food preservation 总被引:1,自引:0,他引:1 下载免费PDF全文
29.
Cndida Lorenzo Teresa Garde-Cerdn Miguel A. Pedroza Gonzalo L. Alonso M. Rosario Salinas 《Food research international (Ottawa, Ont.)》2009,42(9):1281-1286
The purpose of this work was to study the possibility of determination of fermentative volatile compounds in aged red wines using NIR spectroscopy. To achieve this, 240 wines belonging to different geographic zones and elaborated with one or two varieties were analyzed. The volatile compounds were quantified by SBSE-GC–MS. Spectra obtained by NIR were co-related with these values using partial least square (PLS) regression. Calibration and validation statistics showed the quality of the model, after all when is done separately for each of the four geographic zones, and in the case of wines elaborated with two varieties. Consequently, near infrared spectroscopy can be used as an easy and rapid tool to determine fermentative volatile compounds in aged red wines. 相似文献
30.
Alain Tundidor-Camba Nélida Araya Carmen M. González-Henríquez Luis H. Tagle René A. Hauyon Patricio A. Sobarzo Fidel E. Rodríguez-González Ignacio A. Jessop Gonzalo Recabarren-Gajardo Claudio A. Terraza 《应用聚合物科学杂志》2019,136(42):48055
Three new diamines derived from epoxide compounds were synthesized. The preparation of diamine monomers implied the reaction between a phenoxyalkyloxirane (alkyl: H, methyl, isopropyl) and bisphenol A, obtaining the respective aliphatic diols, which produced the corresponding dinitro derivatives. Finally, these derivatives were reduced by using palladium/carbon activated as catalyst and hydrazine as a hydrogen source. Then, six oligomeric poly(ether-azomethine)s (PEAzMs) were obtained from a polycondensation reaction between the new diamines and bis(4-formylbiphenyl-4-yl)dialkylsilane (alkyl: methyl, phenyl) with 84–93% yields. The structural characterization of the diamines and PEAzMs was performed by elemental analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy (1H, 13C, and 29Si). Furthermore, polymers were analyzed by solubility tests, gel permeation chromatography, ultraviolet–visible (UV–vis) spectroscopy, thermogravimetry, and differential scanning calorimetry analysis. The results showed PEAzMs with 2–11 repetitive units, where the design of the monomers allowed to obtain improved solution processability in comparison with previously reported silylated poly(azomethine)s and good thermal stability. Additionally, all samples showed high transparency in the UV–vis region. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 48055. 相似文献