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71.
The creation and characterization of histidine-tagged fusion protein arrays using nitrilotriacetic acid (NTA) capture probes on gold thin films for the study of protein-protein and protein-DNA interactions is described. Self-assembled monolayers of 11-mercaptoundecylamine were reacted with the heterobifunctional linker N-succinimidyl S-acetylthiopropionate (SATP) to create reactive sulfhydryl-terminated surfaces. NTA capture agents were immobilized by reacting maleimide-NTA molecules with the sulfhydryl surface. The SATP and NTA attachment chemistry was confirmed with Fourier transform infrared reflection absorption spectroscopy. Oriented protein arrays were fabricated using a two-step process: (i) patterned NTA monolayers were first formed through a single serpentine poly(dimethylsiloxane) microchannel; (ii) a second set of parallel microchannels was then used to immobilize multiple His-tagged proteins onto this pattern at discrete locations. SPR imaging measurements were employed to characterize the immobilization and specificity of His-tagged fusion proteins to the NTA surface. SPR imaging measurements were also used with the His-tagged fusion protein arrays to study multiple antibody-antigen binding interactions and to monitor the sequence-specific interaction of double-stranded DNA with TATA box-binding protein. In addition, His-tagged fusion protein arrays created on gold surfaces were also used to monitor antibody binding with fluorescence microscopy in a sandwich assay format. 相似文献
72.
Greta Lindwall Peisheng Wang Ursula R. Kattner Carelyn E. Campbell 《JOM Journal of the Minerals, Metals and Materials Society》2018,70(9):1692-1705
Oxygen is always a constituent in “real” titanium alloys including titanium alloy powders used for powder-based additive manufacturing (AM). In addition, oxygen uptake during powder handling and printing is hard to control and, hence, it is important to understand and predict how oxygen is affecting the microstructure. Therefore, oxygen is included in the evaluation of the thermodynamic properties of the titanium-vanadium system employing the CALculation of PHAse Diagrams method and a complete model of the O-Ti-V system is presented. The β-transus temperature is calculated to increase with increasing oxygen content whereas the extension of the α-Ti phase field into the binary is calculated to decrease, which explains the low vanadium solubilities measured in some experimental works. In addition, the critical temperature of the metastable miscibility gap of the β-phase is calculated to increase to above room temperature when oxygen is added. The effects of oxygen additions on phase fractions, martensite and ω formation temperatures are discussed, along with the impacts these changes may have on AM of titanium alloys. 相似文献
73.
Greta M. Eskenazy 《Fuel》1987,66(12):1652-1657
A study of the geochemical behaviour of zirconium (Zr) and afnium (Hf) in several Bulgarian coal deposits has shown that the content of these elements varies considerably, the highest concentrations being found in fragments of coalified woods included in sandstones. Zr and Hf content of the lithotypes of the coals is lower than that of the corresponding whole coal samples. The Zr/Hf ratio for xylain and vitrain is always lower than that for whole coal samples. Zr and Hf are mainly associated with the inorganic matter of the coals and Zrorg and Hforg dominate only in the low ash coals of some deposits. It is assumed that Zrorg and Hforg were leached from the terrigenous minerals supplied to the peat swamp and fixed by the organic matter. These processes result in a slight separation of both elements. 相似文献
74.
Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Andreea Severina Barbulescu Virgil Paunescu 《International journal of molecular sciences》2022,23(21)
Cardiovascular diseases are the leading cause of global mortality. Over the past two decades, researchers have tried to provide novel solutions for end-stage heart failure to address cardiac transplantation hurdles such as donor organ shortage, chronic rejection, and life-long immunosuppression. Cardiac decellularized extracellular matrix (dECM) has been widely explored as a promising approach in tissue-regenerative medicine because of its remarkable similarity to the original tissue. Optimized decellularization protocols combining physical, chemical, and enzymatic agents have been developed to obtain the perfect balance between cell removal, ECM composition, and function maintenance. However, proper assessment of decellularized tissue composition is still needed before clinical translation. Recellularizing the acellular scaffold with organ-specific cells and evaluating the extent of cardiomyocyte repopulation is also challenging. This review aims to discuss the existing literature on decellularized cardiac scaffolds, especially on the advantages and methods of preparation, pointing out areas for improvement. Finally, an overview of the state of research regarding the application of cardiac dECM and future challenges in bioengineering a human heart suitable for transplantation is provided. 相似文献
75.
Rao B Anderson TA Orris GJ Rainwater KA Rajagopalan S Sandvig RM Scanlon BR Stonestrom DA Walvoord MA Jackson WA 《Environmental science & technology》2007,41(13):4522-4528
A substantial reservoir (up to 1 kg ha(-1)) of natural perchlorate is present in diverse unsaturated zones of the arid and semi-arid southwestern United States. The perchlorate co-occurs with meteoric chloride that has accumulated in these soils throughout the Holocene [0 to 10-15 ka (thousand years ago)] and possibly longer periods. Previously, natural perchlorate widely believed to be limited to the Atacama Desert, now appears widespread in steppe-to-desert ecoregions. The perchlorate reservoir becomes sufficiently large to affect groundwater when recharge from irrigation or climate change flushes accumulated salts from the unsaturated zone. This new source may help explain increasing reports of perchlorate in dry region agricultural products and should be considered when evaluating overall source contributions. 相似文献
76.
Nikolina Liović Andre Bratanić Zoran Zorić Sandra Pedisić Anet Režek Jambrak Greta Krešić Tea Bilušić 《International Journal of Food Science & Technology》2021,56(4):1996-2008
The influence of freeze-drying, pasteurisation and high-intensity ultrasound on gastrointestinal stability and antioxidant activity of cultivated blueberry phenolics was investigated. Quantitative and qualitative analyses were done before and after a two-phase in vitro digestion by human digestive enzymes. Antioxidant capacity of blueberry phenolics before and after simulated digestion was evaluated using DPPH and the FRAP assays. After the simulated gastric digestion phase, high stability of phenolics was determined, especially after freeze-drying. During the simulated duodenal digestion, the most affected compounds were anthocyanins. The average loss of quercetin derivatives was 60%, while in all ultrasound-treated samples, a 3-fold increase in the concentration of phenolic acids was observed. The highest antioxidant activity after in vitro gastric and duodenal digestion was determined in a freeze-dried sample. The results after in vitro digestion demonstrated that the use of freeze-drying and high-intensity ultrasound can ensure better gastrointestinal stability and higher antioxidant activity of blueberry phenolics. 相似文献
77.
Adamo Greta Ghidini Chiara DiFrancescomarino Chiara 《Software and Systems Modeling》2021,20(4):1215-1243
Software and Systems Modeling - Business process modelling languages typically enable the representation of business process models by employing (graphical) symbols. These symbols can vary... 相似文献
78.
Oxide nanomaterials are indispensable building blocks for a future nanotechnology, because they offer an infinite variety of structural motifs that lead to their widespread technical application. Therefore, flexible and tunable preparative strategies are required to convert this large family of materials onto the nanoscale. Although hydrothermal syntheses have proven especially suitable for this purpose, their reaction pathways and mechanisms often remain unknown so that they can be difficult to control. In the following, we summarize our comprehensive approach towards nanostructured functional oxides that is based on synthetic parameter optimizations, mechanistic in situ investigations and the characterization of environmentally relevant properties, e.g. in photocatalysis or sensor technology. The connection between preparative morphology control and the resulting materials properties is demonstrated for selected tungstate systems and bismuth-containing oxides. Furthermore, different methods for the in situ monitoring of hydrothermal processes are discussed. 相似文献
79.
Afra Torge Greta Pavone Marijas Jurisic Karola Lima-Engelmann 《Aerosol science and technology》2019,53(1):53-62
Nano-embedded microparticles represent promising carrier systems to tackle the challenges of nanoparticle delivery into the lungs by inhalation. While spray drying is widely used for the incorporation of nanoparticles into microparticles, the template-assisted technique is a novel method to prepare aspherical, cylindrical microparticles composed of nanoparticles. In this work, both techniques were applied to produce both spherical and cylindrical nano-embedded microparticles. For both geometries particles consisting of gelatin nanoparticles, mannitol and leucine were prepared in three different sizes each. Cylindrical microparticles could be prepared with defined dimensions and narrow size distributions, allowing to target a wide range of aerodynamic diameters. The size of spherical microparticles was influenced by the spraying feed concentration, yielding only small differences in geometric and aerodynamic diameters and broad particle size distributions. Regarding the redispersibility of the nano-embedded microparticles, spherical particles showed better disintegration behavior and higher nanoparticle release in comparison to cylindrical particles upon contact with water. The template-assisted technique yielded higher nanoparticle content in contrast to spray drying. In summary, cylindrical particles represent a promising drug delivery system with high potential for later application. However, further improvements in the preparation method are required to enable higher yields and a possible later scale-up.
Copyright © 2018 American Association for Aerosol Research 相似文献
80.
Tamara Posati Claudia Ferroni Annalisa Aluigi Andrea Guerrini Francesca Rossi Francesca Tatini Fulvio Ratto Emanuela Marras Marzia Bruna Gariboldi Anna Sagnella Giampiero Ruani Roberto Zamboni Greta Varchi Giovanna Sotgiu 《大分子材料与工程》2018,303(8)
Mussel‐inspired polydopamine (PD) coating represents a promising route for constructing functional materials and finely tuning or completely changing their surface properties. In this work, a mild and effective method to realize the deposition of PD on keratin‐based films is reported. Reactive oxygen species (ROS), generated by keratin films doped with a photosensitizer, e.g., Azure A (AzA), upon UV–vis and vis‐near IR irradiation, are exploited to obtain the PD coating. Interestingly, the use of vis‐near IR irradiation leads to an increased production of ROS by AzA resulting in a greater PD deposition. Compared to uncoated keratin films, the PD‐keratin coated materials show a granular but homogeneous surface and an increased hydrophilicity, maintaining the ROS generation ability of AzA. This work demonstrates a useful method to effectively and rapidly functionalize with PD materials that are sensitive to temperature, pH and UV light, such as keratin. The proposed strategy allows obtaining new multifunctional biomaterials of potential interest in tissue engineering and drug delivery; in particular, the use of PD‐keratin films for near IR laser bonding of ophthalmic tissue, which is a representative case in wound healing purpose, is shown. 相似文献