Methods to assess physical activity with special reference tomotion sensors and accelerometers

Motion sensors may be applied for the assessment of physical activity. This paper reviews the evolution of these instruments from the mechanical pedometer to the electronic accelerometer. We conclude that for accurate assessment of physical activity under free living conditions the recently introduced accelerometer looks most promising, although little information was available regarding the reliability of these instruments. Subsequently, reliability of an accelerometer with a three-directional sensor was examined. Intrainstrument variation in a bench test was less than 8% during four measurements over a week. Interinstrument variation during treadmill experiments while subjects wore two accelerometers at the same time was on average 22% and was not improved after adjustment for differences found in the bench test. Reproducibility in the treadmill experiment was approximately 76, 85, and 95% at 3, 5, and 7 km/h, respectively. Bench testing revealed that the sensitivity of a piezoelectric element is prone to shifts, probably due to mechanical, electromagnetic, and/or temperature shock, which may be encountered during outdoor application. However, the relevance of the bench test in this study may be questioned, as results did not correspond with the findings in subjects. This needs further investigation.     

Fine structure of the raphe nuclei of sheep and goats]

The medullary raphe system of sheep and goat can be divided in the solitary nuclei raphe magnus and pallidus besides the impaired Nucleus raphe paramedianus. The nucleus raphe magnus begins in the caudal end of the medulla oblongata running far to the pons. On the other hand you can follow the nucleus raphe pallidus and paramedianus only in distinct area near the obex. Different types of neurons are isolated and described in light- and electronmicroscopical investigations. All three nuclei are poor of synapses.     

Deuterated liquid scintillator (NE230) as a fast neutron detectorfor cold-fusion and other research

Deuterated scintillators (NE230) can provide, without time-of-flight, usable neutron energy spectra for neutrons E _n⩾1 MeV. The authors have assembled and calibrated several of these detectors and used them in experiments searching for d+d fusion-neutron emission (E_n, 2.5 MeV) in Pd+D ₂O electrolysis and Ti+D₂ adsorption at LN temperatures. The detectors yielded direct fusion-neutron spectra and set limits in the Pd+D and Ti+D experiments of <7×10^-24 fusion n/s/dd pair and <3×10^-24 fusion n/s/dd pair respectively. In addition, these detectors have been used for in-beam accelerator experiments requiring coincidence between fast neutrons and scattered ions     

Cimaterol and clenbuterol residue analysis by HPLC-HPTLC in liver.

A method has been developed for the analysis of cimaterol and clenbuterol residues in liver, with detection limits of 0.25 micrograms/kg and 0.5 micrograms/kg, respectively. The recovery varied from 55% to 60%. After extraction, a clean-up procedure with Baker-spe C-18 columns was performed. The two chemical compounds of interest were eluted with methanol. Cimaterol and clenbuterol were quantitatively determined by high-performance liquid chromatography (HPLC) using an RP-Select B (5 microns) column and a post-column reaction procedure. The positive results were confirmed by high-performance thin-layer chromatography (HPTLC) as this technique reaches the same level of sensitivity as the HPLC method.     

Ti-silicalite as catalyst for gas-phase ammoximation of cyclohexanone with molecular oxygen

The catalyst behaviour of titanium silicalite, which was found the most active and selective catalyst for liquid-phase ammoximation of cyclohexanone to the corresponding oxime with hydrogen peroxide, was investigated in the gas-phase ammoximation with molecular oxygen in and compared with those of pure silicalite and samples of vanadium and chromium silicalites. The results showed that Ti-silicalite is a selective catalyst not only in the reaction with hydrogen peroxide but also in the reaction with molecular oxygen. However, a big limitation was found in the poor activity of the silicalites which do not allow to reach high oxime yields.     

Aging and everyday memory: A cross-cultural study.

Performance on computer-simulated, everyday memory tasks was found to deteriorate with age in 434 Belgian (aged 14–88 yrs) and 434 American Ss matched on gender and age. This age-related memory decline was reasonably consistent across samples. Difficulties in cross-cultural research and the advantages of ecologically valid measurement instruments are discussed. Instruments included a grocery list selective reminding test, the Wechsler Memory Scale, the Benton Visual Retention Test, and the Wechsler Adult Intelligence Scale (WAIS). (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Evaluating acrosome reaction steps with brightfield and differential interference contrast microscopy techniques.

Acrosomal integrity of bovine spermatozoa was evaluated using naphthol yellow S-erythrosine B stain with bright-field microscopy, .2% glutaraldehyde fixation with Nomarski optics, and live wet smears with Nomarski optics to compare the evaluation techniques. Four ejaculates per two Holstein bulls were enriched through a Percoll gradient, capacitated with heparin, and acrosome-reacted with lysophosphatidylcholine to prepare spermatozoa for in vitro fertilization. Spermatozoa samples were taken after each preparatory step, and percentage of intact acrosomes was evaluated with the stain, .2% glutaraldehyde, and live wet smear. Acrosomal integrity decreased with each preparatory step across all techniques. The decrease in integrity from heparin to lysophosphatidylcholine indicated that all methods detected the acrosome reaction. Technique means across preparation steps showed no differences. Correlations between microscopy techniques were high. The live wet smear used no fixation methods. Both the naphthol yellow S-erythrosine B stain and the .2% glutaraldehyde techniques employed fixation steps that may have introduced artifacts that could influence the data. The live wet smear evaluated by Nomarski optics allowed a comparable evaluation of the acrosome-reacted bovine spermatozoa. Comparing brightfield with differential interference contrast microscopy for detecting the acrosome reaction in bovine spermatozoa showed that both methods were equally accurate.