SPR生物传感系统及其对DNA的检测分析

智能化的SPR生物传感系统采用独特的角向会聚光路代替传统的角度机械扫描方式,采用CCD作为光电检测器件,避免了机械扫描可能带来的误差,可一次完成样品响应曲线的采集分析,提高了检测精度和速度.此外,利用MATLAB软件对信号进行了FIR滤波分析处理,提高了信噪比.利用所研制的系统,分别对DNA分子的体响应以及DNA分子杂交过程进行了检测分析,并且得到了较为满意的结果.     

AFM氮化硅纳米探针研制

在〈100〉晶向硅片上通过各向异性腐蚀形成作为模板的倒金字塔形尖坑, 用LPCVD和PECVD法淀积一层约 1 μm厚的氮化硅膜, 通过等离子刻蚀形成悬臂梁图形,再把抛光玻璃片静电键合到氮化硅膜上, 最后把衬底硅片腐蚀掉,带有金字塔形针尖的悬臂梁就暴露出来,从而构成可用于原子力显微镜(AFM)描绘纳米图像的氮化硅纳米探针.对针尖的尖度和氮化硅膜悬臂梁的热应力补偿进行了深入研讨, 并报道一种特殊而有效的悬臂梁掩模板图形的新设计.制成的氮化硅纳米探针在原子力显微镜上成功地描绘出了纳米图像, 经检测, 其 Z向偏转检测精度优于10-9量级.     

提高力反馈速率陀螺动态特性的优化设计

陀螺带宽窄将影响系统的动态特性.本文采用比例-积分控制和迟后-超前网络相结合的校正方法,通过优化校正网络的参数、合理配置零极点、设计控制回路,使陀螺系统的带宽提高到150 Hz、输出噪声小于10 mV.并通过仿真验证了控制回路设计的合理性.     

基于FPGA的小型光纤光谱仪系统设计

设计了一种小型光纤光谱仪,以FPGA为控制核心,通过CCD实现光电转换,利用USB接口实现光谱仪的供电以及与计算机之间的通讯,采用C++Builder开发计算机软件同时用汞灯谱线进行标定,最后以低压汞灯作为光源对光纤光谱仪进行了性能测试。测试数据表明:该光纤光谱仪在400nm—800nm的有效范围内光谱仪波长扫描精度可达到1nm、像元分辨率可以达到0.12nm/pixel。     

Role of the endothelium in placental dysfunction after fetal cardiac bypass

BACKGROUND: Fetal cardiac bypass causes placental dysfunction, characterized by increased placental vascular resistance, decreased placental blood flow, hypoxia, and acidosis. Vasoactive factors produced by the vascular endothelium, such as nitric oxide and endothelin 1, are important regulators of placental vascular tone and may contribute to this placental dysfunction. METHODS: To investigate the role of the vascular endothelium in placental dysfunction related to fetal cardiac bypass, we studied 3 groups of fetal sheep. In the first group (n = 7) we determined placental hemodynamic responses before and after bypass to an endothelium-dependent vasodilator (acetylcholine), an endothelium-independent vasodilator (nitroprusside), and endothelin 1. In the second group (n = 8) a nonspecific endothelin receptor blocker (PD 145065) was administered and placental hemodynamic values were measured before and after bypass. In the third group (n = 5) endothelin 1 levels were measured before and after bypass. RESULTS: Before fetal cardiac bypass exogenous endothelin 1 decreased placental blood flow by 9% and increased placental resistance by 9%. After bypass endothelin 1 decreased placental flow by 47% and increased resistance by 106%. There was also a significant attenuation of the placental vascular relaxation response to acetylcholine after bypass, whereas the response to nitroprusside was not significantly altered. In fetuses that received the PD 145065, placental vascular resistance increased significantly less than in control fetuses (28% versus 62%). Similarly, placental blood flow decreased significantly more (from 6. 3 +/- 3.1 to 28.3 +/- 10.4 pg/mL; P =.01) in control fetuses than in fetuses receiving PD 145065 (33% versus 20%). Umbilical venous endothelin 1 levels increased significantly in fetuses exposed to fetal bypass but did not change in control fetuses. CONCLUSIONS: The basal endothelial regulatory mechanisms of placental vascular tone were deranged after fetal cardiac bypass. Endothelin receptor blockade, which substantially reduced postbypass placental dysfunction, and other interventions aimed at preserving endothelial function may be effective means of optimizing fetal outcome after cardiac bypass.     

Transgenic animal models: new avenues in cardiovascular physiology

Coryneform bacteria and yeasts of 21 brick cheeses from six German dairies, produced by using undefined ripening cultures, were identified. Arthrobacter nicotianae, Brevibacterium linens, Corynebacterium ammoniagenes, Corynebacterium variabilis and Rhodococcus fascians were found in significant numbers. Out of 148 coryneform isolates 36 could not be identified at the species level. With the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were similar, but the cheeses had clearly different yeast populations. Debaryomyces hansenii and Galactomyces geotrichum prevailed in rennet cheeses while Kluyveromyces marxianus and Pichia membranaefaciens were the main species found in acid cured cheese. The dominance of Yarrowia lipolytica probably indicates an improper yeast population, resulting in poor cheese quality. Some of the species identified are potential candidates for designing a defined ripening culture for rennet red smear cheese.     

Essential functional role of the polysaccharide intercellular adhesin of Staphylococcus epidermidis in hemagglutination

Hemagglutination of erythrocytes is a common property of Staphylococcus epidermidis strains, which is related to adherence and biofilm formation and may be essential for the pathogenesis of biomaterial-associated infections caused by S. epidermidis. In three independent biofilm-producing, hemagglutination-positive S. epidermidis isolates, interruption of the icaADBC operon essential for polysaccharide intercellular adhesin (PIA) synthesis by Tn917 insertions led to a hemagglutination-negative phenotype. An immunoglobulin G fraction of antiserum to PIA greatly reduced hemagglutination. Purified PIA led to a 64-fold decrease of hemagglutination titers of these strains; however, it did not mediate hemagglutination by itself. These observations define PIA as the hemagglutinin of S. epidermidis or at least as its major functional component.     

贵州窄巷口水电站水库渗漏处理中瞬变电磁仪物探勘测研究

贵州猫跳河四级窄巷口水电站水库,岩溶严重渗漏,达30多年.虽然经过多次和多洞口的封堵处理,效果不佳,其渗漏流量仍有15 m3/s左右.针对此情况,提出了分阶段实施封堵的方案.一期工程针对窄巷口河段的3个主要岩溶漏斗,进行局部永久性封堵.为此,进行了补充勘测:采用瞬变电磁仪,在窄巷口库段,以及进水塔前溶洞附近进行了物探勘测,并取得了成果.     

Sperm storage in females of the smooth newt (Triturus v. vulgaris L.): I. Ultrastructure of the spermathecae during the breeding season

Sperm storage in cloacal spermathecae was studied in females of Triturus v. vulgaris collected early in the breeding season in southern England. Females collected in terrestrial situations, presumably unmated, were mated in the laboratory, and the ultrastructure of the transferred sperm and the spermathecae was observed at various intervals after mating. Sperm from a spermatophore cap lodged in a female's cloacal orifice can migrate into spermathecae within 1 hr after mating. Spherical structures on the axial fibers of some sperm in the cap could indicate immaturity. Disorderly clusters of sperm from the cap are still present in the cloacal chamber 12 hr after mating but are absent 24 hr after mating. During storage, sperm often are in tangled masses in the spermathecal tubules. The sperm are coated with spermathecal secretions, and some sperm nuclei were observed embedded in the spermathecal epithelium. Little evidence for spermiophagy early in the breeding season was found. During oviposition, mazes of sperm occur external to the spermathecal orifices, and sperm may be released in this condition onto eggs as they pass through the cloaca. The tangled clusters in which sperm are found from pick-up to oviposition are hypothesized as an adaptation to reduce the effectiveness of sperm competition from the ejaculates of rival males. Additional studies, using the same protocol and covering the entire cycle of sperm storage, are necessary to enable interspecific comparisons leading to phylogenetic hypotheses.