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The net outward current in bursting pace-maker neurones of the snail (Helix pomatia) during sustained and repeated voltage clamp pulses was studied. The properties of currents remaining in cobalt-Ringer or after TEA injection were compared with those in untreated cells. 2. With sustained voltage clamp depolarizations the net outward current first increases to a maximum at 150 msec and then declines to 60% or less of its peak intensity. This depression, which is greater during repetition of short pulses (e.g. 100 msec pulses at 0-5 sec intervals), represents a true decrease in the outward flow of K (designated IK) and is not due to a decreased driving force resulting from extracellular K accumulation. The steady-state current-voltage (I-V) relationship for IK is N-shaped (Heyer & Lux, 1976). 3. A component of IK persists when Ca and Mg in the medium are replaced by Co (ICo-res). With voltage clamp depolarizations ICo-res increases rapidly to a maximum and then partially inactivates with voltage dependent time constants of hundredths or tenths of seconds. Repolarization removes the inactivation. Thus, repeated stimulation with short pulses does not increase the depression of ICo-res-ICo-res (e.g. measured during voltage steps from holding potentials of -50 to near 0 mV) is smaller in test pulses preceded by depolarization and larger in pulses preceded by hyperpolarization. The steady state I-V relationship is not N-shaped. ICo-res is blocked by intracellular injection of tetraethylammonium (TEA). 4. Repeated voltage clamp depolarization to near 0 mV with 100 msec pulses for neurones with large Ca currents in normal Ringer produces a long-term depression which is maximal with 300-400 msec repolarizations (to -50 mV) between pulses. This corresponds with stimulus parameters for the maximum Ca current (Heyer & Lux, 1976). Intracellular injection of Ca2+ (also Ba2+ and Co2+) likewise reduces the total net outward current and especially the delayed outward current under voltage clamp. 5. The component of IK which is removed by Co is identified as Ca dependent and designated IK(Ca). With single voltage clamp pulses IK(Ca) follows the approximate time course and voltage dependence of the slow inward Ca current (Iin slow; Heyer & Lux, 1976). Several lines of evidence suggest that Ca ions moving through the membrane activate IK(Ca). 6. Part of IK cannot be blocked by intracellular TEA injection. In different neurones the magnitude of the IK component resistant to TEA (ITEA-res) is approximately proportional to the relative magnitudes of Iin slow.ITEA-res does not inactivate with sustained depolarization and shows pronounced long-term depression with repetitive stimulation at intermediate intervals and an increased outward current at the onset of the second and subsequent pulses following short repolarizations. The steady-state I-V relationship is N-shaped. ITEA-res is abolished by extracellular Co. 7. A net inward current with low depolarizations can be measured after TEA injection... 相似文献
66.
HD Breidahl 《Canadian Metallurgical Quarterly》1976,5(4):445-451
Dopamine-beta-hydroxylase (DBH), the enzyme responsible for the biosynthesis of norepinephrine from dopamine was assayed in the blood serum of 23 normal adults, 16 patients with congestive heart failure, 19 patients with asymptomatic ischemic heart disease, 4 patients with angina pectoris at rest, 15 patients with essential hypertension, 8 patients with essential hypotension, 5 patients with neurocirculatory asthenia, and 9 normal adults before and after the exercise test. The serum DBH activity varied within a wide range in the control population. The DBH activity increased about 16% after the exercise stress. A tendency to increase DBH activity was shown in the patients with congestive heart failure. There was no significant difference between asymptomatic ischemic heart disease, essential hypertension and normal controls. A lower value was observed in the patients of angina pectoris at rest, while a higher DBH activity was demonstrated in the patients with essential hypotension. There was no significant difference between the neurocirculatory asthenia group and the normal control group. 相似文献
67.
An accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented
which involves the use of18O-labeled internal standards. The method is employed for the determination of hydroperoxides in rat liver microsomes after
aerobic incubation with Fe2+/ADP and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. The amounts found after artificial
microsomal “lipid peroxidation” are almost two orders of magnitude larger than those in microsomes from tetrachloromethaneexposed
animals. 相似文献
68.
Colon atresia is a rare cause of intestinal obstruction in the neonate and requires early diagnosis and prompt surgical treatment. It is impossible in the neonate to differentiate colon atresia from other forms of obstruction at the time of initial presentation. The diagnosis is confirmed roentgenographically, including views of the abdomen and contrast barium enema series. Lesions proximal to the splenic flexure are treated with initial resection of the atretic segment and a primary anastomosis. Those lesions distal to the splentic flexure are managed initally with a diverting loop colostomy with subsequent staged resection and anastomosis. 相似文献
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