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61.
The human neurofibromatosis type 1 (NF1) tumor suppressor protein functions as a Ras-specific guanosine triphosphatase-activating protein, but the identity of Ras- mediated pathways modulated by NF1 remains unknown. A study of Drosophila NF1 mutants revealed that NF1 is essential for the cellular response to the neuropeptide PACAP38 (pituitary adenylyl cyclase-activating polypeptide) at the neuromuscular junction. The peptide induced a 100-fold enhancement of potassium currents by activating the Ras-Raf and adenylyl cyclase-adenosine 3',5'-monophosphate (cAMP) pathways. This response was eliminated in NF1 mutants. NF1 appears to regulate the rutabaga-encoded adenylyl cyclase rather than the Ras-Raf pathway. Moreover, the NF1 defect was rescued by the exposure of cells to pharmacological treatment that increased concentrations of cAMP. 相似文献
62.
Serefeddin Sabuncuo?lu (1385 to 1470?) is known to be the author of the first surgery textbook, namely Cerrahiyyet'ül Haniyye (Imperial Surgery), written in Turkish in 1465. It is the first book to contain colored illustrations of surgical procedures, incisions, and instruments in the Turkish-Islamic medical literature. He was the first man to illustrate and mention introduction of a tube into the pharynx and upper esophagus, removal of foreign bodies in the esophagus by special instruments of his own design, and use of a silver ringlet in a man after tracheotomy. He also described and illustrated reduction of sternal fractures, thoracic puncture through the intercostal space for drainage of empyema cavities, and treatment of rib fractures that have severed the diaphragm. He was a humble, curious, and intelligent surgeon, and also a calligrapher and a miniature artist. 相似文献
63.
Eighteen canine microsatellites 总被引:1,自引:0,他引:1
NG Holmes HF Dickens HL Parker MM Binns CS Mellersh J Sampson 《Canadian Metallurgical Quarterly》1995,26(2):132-133
64.
JE Gaiser E Bloom F Bulos G Godfrey C Kiesling W Lockman M Oreglia DL Scharre C Edwards R Partridge C Peck FC Porter D Antreasyan YF Gu J Irion W Kollman K Strauch K Wacker aA Weinstein D Aschman T Burnett M Cavalli-Sforza D Coyne C Newman HF Sadrozinski D Gelphman R Hofstadter R Horisberger I Kirkbride H Kolanoski K K?nigsmann R Lee A Liberman J O'Reilly A Osterheld B Pollock J Tompkins 《Canadian Metallurgical Quarterly》1986,34(3):711-721
65.
A new method and instrumentation are described for rapid compositional analysis of single-walled carbon nanotube (SWCNT) samples. The customized optical system uses multiple fixed-wavelength lasers to excite NIR fluorescence from SWCNTs individualized in aqueous suspensions. The emission spectra are efficiently captured by a NIR spectrometer with InGaAs multichannel detector and then analyzed by a computer program that consults a database of SWCNT spectral parameters. The identities and relative abundances of semiconducting SWCNTs species are quickly deduced and displayed in graphs and tables. Results are found to be consistent with those based on manual interpretation of full excitation-emission scans from a conventional spectrofluorometer. The new instrument also measures absorption spectra using a broadband lamp and multichannel spectrometers, allowing samples to be automatically characterized by their emission efficiencies. The system provides rapid data acquisition and is sensitive enough to detect the fluorescence of a few picograms of SWCNTs in ~50 μL sample volumes. 相似文献
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69.
The (time-resolved) fluorescence properties of dityrosine in the outermost layer of the spore wall of Saccharomyces cerevisiae were investigated. Steady-state spectra revealed an emission maximum at 404 nm and a corresponding excitation maximum at 326 nm. The relative fluorescence quantum yield decreased with increasing proton concentration. The fluorescence decay of yeast spores was found to be nonexponential and differed pronouncedly from that of unbound dityrosine in water. Analysis of the spore decay recorded at lambda ex = 323 nm and lambda em = 404 nm by an exponential series (ESM) algorithm revealed a bimodal lifetime distribution with maxima centered at tau 1C = 0.5 ns and tau 2C = 2.6 ns. The relative amplitudes of the two distributions are shown to depend on the emission wavelength, indicating contributions from spectrally different dityrosine chromophores. On quenching the spore fluorescence with acrylamide, a downward curvature of the Stern-Volmer plot was obtained. A multitude of chromophores more or less shielded from solvent in the spore wall is proposed to account for the nonlinear quenching of the total spore fluorescence. Analysis of the fluorescence anisotropy decay revealed two rotational correlation times (phi 1 = 0.9 ns and phi 2 = 30.6 ns) or a bimodal distribution of rotational correlation times (centers at 0.7 ns and 40 ns) when the data were analyzed by the maximum entropy method (MEM). We present a model that accounts for the differences between unbound (aqueous) and bound (incorporated in the spore wall) dityrosine fluorescence. The main feature of the photophysical model for yeast spores is the presence of at least two species of dityrosine chromophores differing in their chemical environments. A hypothetical photobiological role of these fluorophores in the spore wall is discussed: the protection of the spore genome from mutagenic UV light. 相似文献
70.
E Shihmanter Y Weisman A Lublin S Mahani A Panshin M Lipkind 《Canadian Metallurgical Quarterly》1998,42(4):829-831
Ten avian serotype 3 paramyxoviruses were isolated for the first time in Israel from passerine and psittacine imported caged birds. The birds were submitted for investigation of an illness characterized by nonspecific signs of weakness, anorexia, vomiting, and sneezing. In addition, only the parakeets developed specific neurologic signs. In bacteriologic and pathologic investigation, cachexia and diarrhea were observed in both groups of birds. In psittacines, considerable alterations were observed in lungs, liver, and spleen. Some nonviral pathogens were occasionally isolated. The isolates appeared to belong to serotype 3b avian paramyxovirus (APMV), the prototype strain of which is APMV-3b/parakeet/Netherlands/449/75. The isolation of APMV-3 viruses from imported caged birds may represent a way of introduction of these viruses into the country. 相似文献