A fast analog mean-timer

A circuit is described that determines the average of the transit time of light from an event in a scintillation counter to the two ends of that counter. The design offers superior time resolution and a novel gating scheme that significantly reduces circuit dead-time.     

Activating and inactivating mutations in LH receptors

In rats, galanin modulates luteinizing hormone (LH) secretion, and gonadotropin-releasing hormone (GnRH) neurons provide a possible source of this galanin. To understand galanin's physiological role in GnRH neurons, we used double-label in situ hybridization and computerized image analysis to examine the regulation of galanin message in GnRH neurons. We found that galanin gene expression in GnRH neurons is regulated by sex steroids, induced coincident with the LH surge, and persists well after the completion of the LH surge, and that the induction of galanin message in GnRH neurons coincident with the LH surge is sexually differentiated neonatally. We postulate that the rise in galanin gene expression in GnRH neurons at the time of the LH surge serves to replenish galanin released with GnRH that is needed for the production of the LH surge, or that galanin is involved in physiological events that occur subsequent to the LH surge.     

Conceptus, progesterone, and breed effects on uterine protein secretion in swine

This experiment consisted of the following treatment-breed groups: 1) White crossbred gilts, 2) White crossbred gilts treated with progesterone (200 mg/d in corn oil given on d 2 and 3 after estrus), and 3) Chinese Meishan gilts. Pregnant and nonpregnant gilts (n=3 to 6) from each treatment-breed combination were assigned to be slaughtered on d 10, 11, 12, 13, and 15. At slaughter each uterine horn was flushed with 20 mL of minimal essential medium. Uterine flushings were assayed for total protein, acid phosphatase, uteroferrin, retinol-binding protein, and oxytocin. Uterine flush total protein was increased by progesterone treatment, was unaffected by pregnancy status, and was less in Meishans. Similar patterns were found for retinol binding protein and uteroferrin, except that uteroferrin was greater in pregnant than in nonpregnant gilts. Oxytocin was greater in pregnant than in nonpregnant gilts, was not influenced by progesterone treatment, and was similar in Meishan and in White crossbred gilts. These results indicate that the conceptus does not influence secretion of either total protein or retinol binding protein during pregnancy and that the onset of secretion of these uterine proteins may be controlled by progesterone. The presence of the conceptus is associated with increased uteroferrin and oxytocin production. The decreased secretion of uterine proteins in Meishan gilts may partially explain the slower embryonic development that has been reported for this breed.     

Performance characteristics of a whole-body PET scanner

METHODS: This study characterizes the performance of a newly developed whole-body PET scanner (Advance, General Electric Medical Systems, Milwaukee, WI). The scanner consists of 12,096 bismuth germinate crystals (4.0 mm transaxial by 8.1 mm axial by 30 mm radial) in 18 rings, giving 35 two-dimensional image planes through an axial field of view of 15.2 cm. The rings are separated by retractable tungsten septa. Intrinsic spatial resolution, scatter fraction, sensitivity, high count rate performance and image quality are evaluated. RESULTS: Transaxial resolution (in FWHM) is 3.8 mm at the center and increases to 5.0 mm tangential and 7.3 mm radial at R = 20 cm. Average axial resolution decreases from 4.0 mm FWHM at the center to 6.6 mm at R = 20 cm. Scatter fraction is 9.4% and 10.2% for direct and cross slices, respectively. With septa out, the average scatter fraction is 34%. Total system sensitivity for true events (in kcps/(microCi/cc)) is 223 with septa in and 1200 with septa out. Dead-time losses of 50% correspond to a radioactivity concentration of 4.9 (0.81) microCi/cc and a true event count rate of 489 (480) kcps with septa in (out). Noise-equivalent count rate (NECR) for the system as a whole shows a maximum of 261 (159) kcps at a radioactivity concentration of 4.1 (0.65) microCi/cc with septa in (out). NECR is insensitive to changes in lower gamma-energy discrimination between 250-350 keV. CONCLUSIONS: The results show the performance of the newly designed PET scanner to be well suited for clinical and research applications.     

Non-transferrin iron uptake by trophoblast cells in culture. Significance of a NADH-dependent ferrireductase

BACKGROUND: Glucocorticoids have been linked to self-administration of a wide range of drugs in animals and are increased endogenously by chronic nicotine intake. Corticosteroids have also been shown to regulate nicotine receptor sensitivity and to be involved in behavioral sensitization to nicotine. METHODS: Cortisol levels and cortisol suppression in response to dexamethasone were measured in a sample of smokers participating in a smoking cessation treatment trial. RESULTS: Cortisol levels dropped significantly during the early quitting process (2 weeks post-quit) and returned to a level below baseline 1 month post-quit. The magnitude of the initial drop in cortisol was strongly related to post-quit distress and marginally predictive of abstinence. Neither baseline nor post-quit changes in percent cortisol suppression after dexamethasone were related to abstinence success or withdrawal distress. CONCLUSIONS: Withdrawal from cigarette smoking is marked by a reduction in cortisol levels that appears to be related to the degree of distress experienced during the early quitting period. Further work is needed to determine whether withdrawal-related cortisol changes or distress are predictive of abstinence success.     

Hyoid bone malformation confirmed by 3-dimensional computed tomography

We describe the case of a young woman with pain on turning her head attributable to a malformation of the hyoid bone. Diagnosis was established using spiral computed tomography with the patient's neck in the position of greatest discomfort and with 3-dimensional reconstruction of the hyoid bone. Prior conventional radiography and magnetic resonance imaging did not aid in finding a diagnosis. After surgical removal of both greater cornua of the hyoid bone there was a complete relief of symptoms.     

The effect of acoustic diffraction and acoustic attenuation on the dynamic range of acoustooptic Bragg cells

A three-dimensional model for the propagation of finite acoustic waves in nonlinear media is developed. This model implicitly includes the effects of acoustic attenuation and divergence due to diffraction. The generation of intermodulation products in the case of a two-tone input signal is numerically analyzed. It is found that acoustic diffraction can have a significant effect on the dynamic range of a Bragg cell if the acoustic field extends well into the Fraunhofer region. Inclusion of the effect of diffraction in the model predicts a dynamic range that can be considerably larger than the value obtained by using the infinite plane wave assumption. It is shown that acoustic attenuation significantly reduces the level of the acoustic intermodulation products relative to the level of the fundamental modes. This also increases the dynamic range. The influence of these effects on design considerations for Bragg cells is discussed.     

A Realistic Computer-Simulated Brain Phantom for Evaluation of PET Charactenstics

To evaluate accurately the imaging characteristics of positron emission tomography (PET), a realistic computer-simulated brain phantom was developed. A cross-sectional slice from a human cadaver brain was chosen for its combination of gray matter, white matter, and cerebrospinal fluid (CSF) regions. The slice was photographed and digitized into a gray-level image with a video digitizer, boundary edges were located around cerebral structures in the digitized image, and each structural region was assigned a uniform pixel value dependent on both the cerebral parameter (e.g., blood flow, oxygen uptake, metabolic rate) under investigation and the type of structure (gray matter, white matter, CSF). Line integrals through the regions were generated at various angular and transverse positions according to specific physical characteristics (such as detector line-spread function) of a tomographic scanner configuration to create a set of simulated but realistic projection measurements. The set of projection measurements can be processed with any standard reconstruction program to create a tomographic image to reveal the effects of various PET characteristics. Investigations with this computer-simulated brain phantom have demonstrated its usefulness for examining the interrelations among neuroanatomical structure volume, tomographic spatial resolution, partial volume effect, and nonlinear parameter estimation. Transportability of the simulated phantom and the procedure to other medical imaging environments is described, and limitations of this simulation procedure are discussed.     

Sparse decompositions for exploratory pattern analysis

We define and verify the utility of a pattern analysis procedure called sparse decomposition. This technique involves sequentially ``peeling' sparse subsets of patterns from a pattern set, where sparse subsets are sets of patterns which possess a certain degree of regularity or compactness as measured by a compactness measure c. If this is repeated until all patterns are deleted, then the sequence of decomposition ``layers' derived by this procedure provides a wealth of information from which inferences about the original pattern set may be made. A statistic P is derived from this information and is shown to be powerful in detecting clustering tendency for data in reasonably compact sampling windows. The test is applied to both synthetic and real data.     

Elastic Properties and Microcracking Behavior of Particulate Titanium Diboride–Silicon Carbide Composites

The spontaneous microcracking of particulate TiB₂–SiC composites is studied as a function of TiB₂ volume fraction. The degree of microcracking was examined by measuring elastic properties from room temperature to 1300°C. The results showed that only one composition contains microcracks. All other compositions did not microcrack regardless of TiB₂ volume fraction. This was attributed to the difference in the sintering aids. In particular, the Al₂O₃ sintering aid needed in these compositions had reacted with SiO₂ to form an amorphous grain boundary phase that allowed residual stresses to relax by viscous flow at moderate to high temperatures. The existence of this amorphous grain boundary phase was directly observed by transmission electron microscopy.