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81.
F F?ndrich AM Waaga J Schr?der E Schweizer A Schmid S Jacob HH Wacker P Schroeder 《Canadian Metallurgical Quarterly》1995,27(2):1553-1554
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HE Vi?tor F Klumper RJ Meerman A Brand HH Kanhai 《Canadian Metallurgical Quarterly》1998,18(4):325-331
Histamine is an important mediator in allergic reactions, gastric acid secretions, and neurotransmission in the central nervous system. Basophils and mast cells are the main sources of histamine, which is formed from L-histidine by histidine decarboxylase (HDC). However, the regulatory mechanism of HDC in these cells remains unclear. We examined the regulation of HDC activity and gene expression using a unique human mast cell line, HMC-1, after stimulation with phorbol 12-myristate 13-acetate (PMA) or ionomycin. HDC activity was increased from 52.1+/-0.4 (mean+/-standard deviation) to 154+/-6.9, or 105.6+/-6.2 pmol/min/mg protein (n = 3), 4 hours after stimulation with PMA (10 ng/mL) or ionomycin (10[-6] M). Although actinomycin D had no effect on this increase, cycloheximide completely inhibited the increase caused by these stimuli. The population of HMC-1 cells containing HDC protein was increased after stimulation with either PMA or ionomycin as evaluated by immunocytochemical analysis with anti-HDC antibody as a marker. HMC-1 constitutively expressed HDC mRNA, and its level was not increased with these stimuli. These results suggest that the increase of HDC activity in HMC-1 induced by PMA or ionomycin is regulated at the translational level. 相似文献
85.
C Perka K Lindenhayn HH Heilmann M Sittinger M Muschik 《Canadian Metallurgical Quarterly》1996,134(6):562-572
Full thickness defects (diameter 1,7 mm; depth 2,5 mm) were created mechanically in articular cartilage and subchondral bone of the condyles of tibiotarsal joints of 9-month old chickens. This full-thickness defects were repaired with cultured allogenic embryonic chick epiphyseal chondrocytes from the tibiae and femura of 10-days-old chicken embryos. The cells were embedded in a collagen-fibrinogen-matrix. Controls were similarly operated, but received either no treatment or implants the delivery substance only. Healing of the defects was observed macroscopically, histologically, histochemically and histomorphometrically after 3, 12 and 24 weeks. This graft was successfully transplanted in mechanically induced defects in 80%. The resulting hyaline cartilage was structurally reorganized according to the host pattern and under the influence of environmental conditions. The articular zone preserved it's cartilaginous phenotype, whereas the subchondral regions were transformed into bone. 12 weeks after the operation the defects in the experimental group were completely filled. In all instances in this group, there was an initial extreme increase of mitotic rate and cell number. After 24 weeks normal and subnormal values were founded. The defects in the control groups healed with fibrocartilage. Our results showed, that only the defects in the experimental group were completely filled with reparative hyaline cartilage tissue. In the present study the mixture of cultured allogenic embryonic chondrocytes and a collagen-fibrinogen-matrix was used successfully as a transplant for repairing defects in articular cartilage of chickens. Thus allogenic transplantation of cultured embryonal chondrocytes appears to be one of the most promising methods for the restoration of articular cartilage. 相似文献
86.
Whether reexposure of varicella-immune persons to varicella-zoster virus would protect against or predispose to development of zoster was analyzed. The rate of zoster in 511 leukemic recipients of varicella vaccine who had 1 or > 1 dose of varicella vaccine and in those who did or did not have a household exposure to varicella was determined. A Kaplan-Meier life-table analysis revealed that the incidence of zoster was lower in those given > 1 dose of vaccine (P < .05). A Cox proportional hazards analysis showed that both household exposure to varicella and receipt of > 1 dose of vaccine were highly protective (P < .01) against zoster. Thus, the risk of zoster is decreased by reexposure to varicella-zoster virus, either by vaccination or by close exposure to varicella. 相似文献
87.
本文以CAI课件的开发制作过程遇到的一些关键的技术问题加以探讨.并结合实际制作介绍了一些技艺方法、 相似文献
88.
冯国民 《农村实用工程技术(绿色食品)》2005,(9):55-55
波尔多液的配制。波尔多液是应用范围很广的杀菌剂,它是由硫酸铜、石灰和水混配而成的药液.配制原料:硫酸铜为蓝色的块状结晶,石灰以块状的生石灰为好.如果没有生石灰,使用粉状的消石灰也可,但用量要加大到30%. 相似文献
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90.
一人脉对照 1 日本现代建筑师溯流 引领日本现代建筑进人成熟时期的领袖人物非丹下健三莫属。由师从丹下的模文彦(1928年-)、黑川纪章(1934年-)以及大高正人(1923年-)、菊竹清训(1928年-)等4人在1960年代创导的新陈代谢运动,使主流的欧美建筑界开始认真关注一个亚洲的小国——日本的建筑活动。可以说新陈代谢运动是日本现代建筑发展的基石也是随后其建筑发展的原动力。 相似文献