Application of nested PCR and mass spectrometry for DNA-based virus detection: HBV-DNA detected in the majority of isolated anti-HBc positive sera

DNA preparations from three different groups of serum samples were examined for HBV-DNA via a nested polymerase chain reaction assay (lower detection limit: 10 viral genomes in 100 microliters serum): Group I consisted of 11 uninfected control sera, group II consisted of sera obtained from 11 HBV infected patients and group III consisted of 21 isolated anti-HBc positive samples. The 21 samples from group III were HBV-DNA negative according to a conventional non-nested PCR assay and hybridization with a 32P-labelled probe. Using nested PCR and mass spectrometry, HBV-DNA was detected in none of group I and in all of group II samples. In 11 out of 21 (52%) of the isolated anti-HBc positive sera from group III, HBV-DNA was detected. No correlation was observed between HBV-DNA positivity and anti-HBc titers. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry provided a fast, sensitive and non-radioactive assay for the detection of PCR products without the need for gel electrophoresis or hybridization with labelled probes.     

Prenatal diagnosis of trisomy 13 on fetal cells obtained from maternal blood after minor enrichment

In a pilot study to establish fetal nucleated red blood cell (NRBC) detection in maternal blood, trisomy 13 was diagnosed by FISH analysis at 11 weeks' gestation. The NRBCs were detected after a single-step ficoll density gradient enrichment. In blood samples taken both before and after CVS, 52 and 80 NRBCs, respectively, were found to be positive for fetal haemoglobin. In 47 per cent of these cells, FISH analysis for X and Y chromosomes confirmed the fetal sex. Moreover, 48 per cent of these NRBCs showed three fluorescent signals for a chromosome 13 probe, which confirmed the diagnosis of trisomy 13, previously detected at CVS karyotyping. This is the first report of non-invasive prenatal diagnosis of trisomy 13, i.e., pre-CVS, in the first trimester. The high number of fetal NRBCs detected indicates a connection with aneuploidy, probably due to early impairment of the feto-maternal barrier.     

软件过程改进方法研究与实施

信息化作为军事变革的本质和核心,是高新武器装备发展的方向,而软件又是信息化武器装备的中枢神经。文章介绍了军用软件改进方法的研究与实施,从软件的实施规划、过程定义、试点实施、全面推广入手,从而提高了军用软件的质量。     

浅析工业设计教育中视知觉修养的培养

本文首先阐述视知觉修养对产品设计的推动作用,进而从心理学角度,分析视觉笔记在设计心理学层面如何改变学生对产品设计的认知,最后论述教学训练过程中应注意的一些问题。     

一种动态Q值多标签识别算法的性能分析

EPCglobal组织发布的第二代超高频射频识别空中接口协议EPC-C1G2协议可同时识别多个标签,该协议采用时槽ALOHA算法来解决多标签识别时产生的碰撞问题。文章首先介绍了EPC-C1G2协议多标签碰撞解决所需的参数和命令,指出协议中的Q值决定了解决碰撞时所用的时槽数。时槽数越多,标签越不容易碰撞,但识别时间越长,因此,恰当地选择Q值可以在标签识别时间和识别标签数之间找到最佳点。文中同时给出了一种动态Q值算法,该算法可根据标签碰撞解决的实际情况动态调整Q值,使标签识别更高效。文章最后对这种多标签识别流程的性能进行了仿真分析。     

脉冲噪声环境下宽带循环平稳信号DOA估计算法

针对传统二阶循环相关算法在脉冲噪声环境中的显著退化问题,本文以α稳定分布为噪声模型,提出基于分数低阶循环相关的波达方向(Direction of arrival,DOA)估计算法。利用分数低阶循环相关的相移特性,将宽带循环平稳信号的DOA估计问题转化为"中心频率"为ε的窄带问题,解决了宽带情况下DOA估计困难的问题。计算机仿真结果进一步验证了此算法的有效性,且性能优于传统SC-SSF(Spectral correlation signal subspacefitting)算法。     

基于仿人足球机器人协作定位技术研究

本文综述多机器人协作定位的研究现状,分析比较当前该领域的主要研究方向,介绍实现机器人协作定位的主要研究方法和关键技术,并总结多机器人协作定位面临的挑战和发展趋势.     

中医方剂与图集的转换方法

中药复方数据挖掘研究是将我国丰富的中药信息资源和现代最新信息技术相结合的重要内容,它意味着基于中医理论,利用知识发现技术,对传统中药新药、中医组方理论及规律、中药作用机制、有效成分构效关系等多个方面进行全面、系统的研究。图作为一种经典的数据结构,被广泛用于复杂数据结构分析与建模。文章探讨了图建模技术并实现了中医方剂与图集的转换。     

基于Transformer实现文本导向的图像编辑

为方便非专业用户修图,提出一种基于Transformer的图像编辑模型TMGAN,使用户可通过自然语言描述自动修改图像属性。TMGAN整体框架采用生成对抗网络,生成器采用Transformer编码器结构提取全局上下文信息,解决生成图像不够真实的问题;判别器包含基于Transformer的多尺度判别器和词级判别器两部分,给生成器细粒度的反馈,生成符合文本描述的目标图像且保留原始图像中与文本描述无关的内容。实验表明,此模型在CUB Bird数据集上,IS（inception score）、FID（Fréchet inception distance）以及MP（manipulation precision）度量指标分别达到了9.07、8.64和0.081。提出的TMGAN模型对比现有模型效果更好,生成图像既满足了给定文本的属性要求又具有高语义性。