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M Steinhardt HH Thielscher T von Horn R von Horn K Ermgassen D Smidt 《Canadian Metallurgical Quarterly》1996,103(4):136-141
Dairy calves and their dams from the dairy herd of the institute were used for this investigation. Calvings were watch carefully and jugular venous blood samples taken immediately after delivery from the cow and the calf and in the calf at 24 hours and at 48 hours of postnatal age as well were analyzed for T4, FT4, T3 and FT3 by luminescence enzyme immunoassay (LEIA). Higher thyroid values in calves than in their dams could be found increasing short after birth and reaching their highest level within 24 hours of postnatal life. The individual thyroid hormone values at birth and at 24 hours and at 48 hours of age are well correlated meaning the changes during the first days are specific for each calf. Strong correlation between the thyroid hormone values of the cows and those of the calves could be found. Mean values of thyroid hormones were significantly higher in female than in male calves all time but not different in the dams of these calves. 相似文献
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Posada J.F. Liou J.J. Miller R.N. 《IEEE transactions on instrumentation and measurement》1991,40(5):836-841
Population growth results in an increasing demand on water supply. Usually, growers determine how much water is needed to irrigate their fields on the basis of the amount of time elapsed since the last irrigation cycle. A soil moisture sensor enables the users (or an automated irrigation system) to monitor the relative soil moisture constantly at many different locations throughout the field in order to schedule irrigation cycles more precisely. An automated data acquisition system for measuring the characteristics of a soil moisture sensor under various moisture conditions is presented. Based on the measurement data, an empirical model for the soil moisture sensor is also developed. A comparison of the model and experimental data is included 相似文献
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C Jurinke B Z?llner HH Feucht D van den Boom A Jacob S Polywka R Laufs H K?ster 《Canadian Metallurgical Quarterly》1998,14(3):97-102
DNA preparations from three different groups of serum samples were examined for HBV-DNA via a nested polymerase chain reaction assay (lower detection limit: 10 viral genomes in 100 microliters serum): Group I consisted of 11 uninfected control sera, group II consisted of sera obtained from 11 HBV infected patients and group III consisted of 21 isolated anti-HBc positive samples. The 21 samples from group III were HBV-DNA negative according to a conventional non-nested PCR assay and hybridization with a 32P-labelled probe. Using nested PCR and mass spectrometry, HBV-DNA was detected in none of group I and in all of group II samples. In 11 out of 21 (52%) of the isolated anti-HBc positive sera from group III, HBV-DNA was detected. No correlation was observed between HBV-DNA positivity and anti-HBc titers. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry provided a fast, sensitive and non-radioactive assay for the detection of PCR products without the need for gel electrophoresis or hybridization with labelled probes. 相似文献