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111.
Narrow bandwidth bandstop filters or ‘notch filters’ are frequently required in both communication and electronic warfare systems. In the latter case the ability to switch into an ‘allpass’ state with a minimum level of loss and time delay distortion is vital. Furthermore, in both cases a level of 20-30 dB rejection is normally required. These factors suggest that the ‘hybrid notch filter’? proposed in this paper is an optimum solution for low-loss designs. Design techniques and experimental results for two fixed tuned filters for cellular base station applications are presented. 相似文献
112.
Rosenwald AG Rhodes MA Van Valkenburgh H Palanivel V Chapman G Boman A Zhang CJ Kahn RA 《Yeast (Chichester, England)》2002,19(12):1039-1056
To examine the functions of the Arf-like protein, Arl1p, in Saccharomyces cerevisiae, a null allele, arl1delta::HIS3, was constructed in two strains. In one background only, loss of ARL1 resulted in temperature-sensitive (ts) growth (suppressed on high-osmolarity media). Allelic variation at the SSD1 locus accounted for differences between strains. Strains lacking ARL1 exhibited several defects in membrane traffic. First, arl1delta strains secreted less protein as measured by TCA-precipitable radioactivity found in the media of [(35)S]-labelled cells. A portion of newly synthesized carboxypeptidase Y (CPY) was secreted rather than correctly targeted to the vacuole. Uptake of the fluid-phase marker, lucifer yellow, was reduced. All these phenotypes were exacerbated in an ssd1 background. The ts phenotype of the arl1deltassd1 strain was suppressed by YPT1, the yeast Rab1a homologue, suggesting that ARL1 and YPT1 have partially overlapping functions. These findings demonstrate that ARL1 encodes a regulator of membrane traffic. 相似文献
113.
We investigate explicit segment duration models in addressing the problem of fragmentation in musical audio segmentation.
The resulting probabilistic models are optimised using Markov Chain Monte Carlo methods; in particular, we introduce a modification
to Wolff’s algorithm to make it applicable to a segment classification model with an arbitrary duration prior. We apply this
to a collection of pop songs, and show experimentally that the generated segmentations suffer much less from fragmentation
than those produced by segmentation algorithms based on clustering, and are closer to an expert listener’s annotations, as
evaluated by two different performance measures.
Editor: Gerhard Widmer 相似文献
114.
J. D. Rhodes 《International Journal of Circuit Theory and Applications》1973,1(4):363-371
The conventional method of designing stepped impedance transmission line networks from gain functions requires the use of a factorization process to obtain a passive input impedance, and then the application of Richards Theorem to determine the element values. A new technique is presented based upon the determination of the Fourier coefficients of the return loss function from which the element values may be obtained immediately for the minimum phase reflection coefficient without any factorization process or synthesis procedure. It is believed that this new technique may be extended directly to the design of mixed lumped-distributed transmission line networks whereas the conventional approach may not. 相似文献
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117.
High avidity CTLs for two self-antigens demonstrate superior in vitro and in vivo antitumor efficacy
HJ Zeh D Perry-Lalley ME Dudley SA Rosenberg JC Yang 《Canadian Metallurgical Quarterly》1999,162(2):989-994
A majority of the human tumor-associated Ags characterized to date are derived from nonmutated "self"-proteins. Little is currently understood about the nature of the self-reactive lymphocytes that recognize these Ags. We recently characterized two nonmutated tumor-associated Ags for the B16 murine melanoma: tyrosinase-related protein-2 (TRP-2) and the endogenous retroviral envelope protein, p15E. We previously reported that both TRP-2 and p15E reactive CTL could be detected in the spleens of naive animals after a single in vitro stimulation using 10(-5)-10(-6) M of the appropriate Kb-binding 9-amino acid epitope. In this report we show that the CTL found in naive animals are low avidity lymphocytes, that respond only to high concentrations of peptide in vitro. We demonstrate that titration of in vitro-stimulating peptide to limiting concentrations distinguishes qualitative differences in the lymphocyte reactivity to these two Ags between vaccinated and unvaccinated animals. We further demonstrate that in vitro expansion of CTL in either high or low concentrations of stimulating peptide generated CTL cultures with different avidities for the relevant epitopes. CTL expanded in low concentrations demonstrated higher avidity for peptide-pulsed targets and better tumor recognition, when compared to CTL generated in the presence of high concentrations of Ag. More importantly, high avidity CTL demonstrated superior in vivo antitumor activity. These results demonstrate that qualitative differences in the CTL that recognize these two self-Ags are critically important to their in vitro and in vivo anti-tumor efficacy. 相似文献
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119.
We have identified a Mg(2+)-dependent endonuclease activity from human B lymphoblastic IM9 cell lysates and nuclei using autodigestion method and DNA-native-polyacrylamide gel electrophoresis (DNA-native-PAGE) nuclease assay system. The level of the endonuclease activity in cell lysates was significantly decreased at certain stage by treatment of the cells with cycloheximide. However, the enzyme activity consistently remained for over 12 hours in the isolated nuclei of the apoptotic IM9 cells. The Mg(2+)-dependent endonuclease isolated from the nuclei by native-PAGE elution was able to catalyze the conversion of supercoiled plasmid DNA into linear form. This particular endonuclease activity was not detected in cycloheximide treated-U937 cells. Several lines of experimental evidence suggest that the Mg(2+)-dependent endonuclease localized in the nucleus may be responsible for the DNA fragmentation of apoptotic IM9 cells. 相似文献
120.