Comparison of molecular changes in lung cancers in HIV-positive and HIV-indeterminate subjects

CONTEXT: Human immunodeficiency virus (HIV) infection has been associated with an increasing incidence of malignancy, and HIV-infected persons have an increased incidence of primary lung carcinoma compared with the general population. OBJECTIVE: To investigate the molecular changes present in HIV-associated lung tumors and compare them with those present in lung carcinomas arising in HIV-indeterminate subjects ("sporadic tumors"). DESIGN: Convenience sample. SUBJECTS: Archival tissues from 11 HIV-positive persons and from 35 persons of indeterminate HIV status. SETTING: University-based medical centers and affiliated hospitals. MAIN OUTCOME MEASURES: Analysis of frequency of loss of heterozygosity (LOH) and microsatellite alteration (MA) using polymerase chain reaction and 16 polymorphic microsatellite markers at 8 chromosomal regions frequently deleted in lung cancer. Presence of HIV and human papillomavirus (HPV) sequences. RESULTS: The overall frequency of LOH at all chromosomal regions tested and the frequencies at most of the individual regions were similar in the 2 groups. Frequency of MA present in the HIV-associated tumors (0.18) was 6-fold higher than in sporadic tumors (0.03) (P<.001). At least 1 MA was present in 10 (91%) of 11 HIV-associated tumors vs 17 (48%) of 35 sporadic tumors (P=.02). Molecular changes were independent of tumor stage and gender. HIV and HPV sequences were not detected in the HIV-associated lung carcinomas. CONCLUSIONS: Microsatellite alterations, which reflect widespread genomic instability, occur at greatly increased frequency in HIV-associated lung carcinomas. Although the mechanism underlying the development of increased MAs is unknown, it may play a crucial role in the development of many HIV-associated tumors.     

Quantal release at visualized terminals of a crayfish motor axon: intraterminal and regional differences

Synaptic transmission was measured at visualized terminal varicosities of the motor axon providing the sole excitatory innervation of the "opener" muscle in walking legs of crayfish (Procambarus clarkii Girard). Two questions were addressed: 1) How uniform is quantal emission at different locations along terminals innervating a single muscle fiber, and 2) can differences in quantal emission account for the different excitatory postsynaptic potential (EPSP) amplitudes generated by terminals localized in defined regions of the muscle? Extracellular "macropatch" electrodes were placed over individual varicosities, viewed after brief exposure to a fluorescent dye, and synaptic currents were recorded to determine quantal content of transmission. Along terminals supplying a single muscle fiber, nonuniform release was found: Varicosities closer to the point of origin of the terminal branch released more transmitter than those located more distally. Quantal content was higher for varicosities of the muscle's proximal region (where large EPSPs occur) than for varicosities of the central region (where small EPSPs occur). The probability of transmitter release per synapse is estimated to be greater for the proximal varicosities. At low frequencies of stimulation, quantal content per muscle fiber is two to four times larger in the proximal region. Taken in conjunction with a twofold higher mean input resistance for the proximal muscle fibers, the difference in quantal content can account for a four- to eightfold difference in EPSP amplitude. The observed mean EPSP amplitude is at least eight times larger in the proximal region. We discuss factors contributing to differences in EPSP amplitudes.     

Metrics for MT evaluation: evaluating reordering

Translating between dissimilar languages requires an account of the use of divergent word orders when expressing the same semantic content. Reordering poses a serious problem for statistical machine translation systems and has generated a considerable body of research aimed at meeting its challenges. Direct evaluation of reordering requires automatic metrics that explicitly measure the quality of word order choices in translations. Current metrics, such as BLEU, only evaluate reordering indirectly. We analyse the ability of current metrics to capture reordering performance. We then introduce permutation distance metrics as a direct method for measuring word order similarity between translations and reference sentences. By correlating all metrics with a novel method for eliciting human judgements of reordering quality, we show that current metrics are largely influenced by lexical choice, and that they are not able to distinguish between different reordering scenarios. Also, we show that permutation distance metrics correlate very well with human judgements, and are impervious to lexical differences.     

Illness and injury in family day care: a seasonal survey

Many mothers with children under age 6 are employed and it is not usually feasible for a parent to stay home with a child who is mildly ill. Such ill children likely remain in child day care programs. The extent to which this occurs and the management of these children in family day care was studied. Over the course of a year, 1 to 3 visits were made to 714 family day care homes in order to survey providers. With enrollments ranging between 0 and 18 children per day care home at the time of each visit, information on 3,630 "child enrollment days" was collected. The median age of children in care was 2 years. An average of 16% of all children were ill on any one day (with seasonal variation); 1% were injured. Of ill children, 82% attended day care that day, 49% had contacted a physician about that illness, and 28% were administered a medication at the day care setting. Twenty-one percent of children receiving medication in day care had no contact with a physician for that illness. These data show that mild childhood illnesses are routinely managed by child day care providers. Physicians who traditionally limit their illness-management education to parents need to recognize the health education and consultation needs of day care providers.     

Thermal properties of highly birefringent optical fibers and preforms

Temperature cycling of highly birefringent optical fibers and preforms has been used to investigate the thermal properties of bow-tie and elliptically clad structures. The thermal hysteresis of the birefringence is shown to be a direct consequence of the thermal history of the fiber or preform and has been related to volume changes in the stress-producing borosilicate sections. Annealing increases the axial stress as well as the stress anisotropy and hence the birefringence. Increases of up to a factor of 2 in the birefringence on suitable thermal treatment indicate a new method for further improvement of high birefringence fibers. The implications of the results in the design, fabrication, and use of such fibers are discussed.     

Maternal influences on daughters' restrained eating behavior.

This study examined whether mothers' preoccupation with their own weight and eating was linked to daughters' restrained eating behavior. Participants included 173 non-Hispanic, White mother-daughter dyads, measured longitudinally when daughters were ages 5, 7, 9, and 11. Mothers who were preoccupied with their own weight and eating reported higher levels of restricting daughters' intake and encouraging daughters to lose weight over time. Mothers' encouragement of daughters' weight loss was linked to daughters' restrained eating behavior; this relationship was partially mediated by daughters' perception of maternal pressure to lose weight. These findings suggest that mothers' preoccupation with weight and eating, via attempts to influence daughters' weight and eating, may place daughters at risk for developing problematic eating behaviors. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Mood-Induced Increases in Alcohol Expectancy Strength in Internally Motivated Drinkers.

This study investigated whether exposure to musical mood induction procedures (MMIP) differentially increases the strength of specific alcohol expectancies for coping motivated (CM) versus enhancement motivated (EM) drinkers. Participants were 86 undergraduates who had elevated scores on either the CM or EM subscale of the Drinking Motives Questionnaire (M. L. Cooper, 1994). Participants were randomly assigned to either a positive or negative mood condition. The Alcohol Craving Questionnaire (E. G. Singleton, S. T. Tiffany, & J. E. Henningfield, 1994) was administered at baseline and after MMIP to assess phasic changes in alcohol expectancy strength. Consistent with hypotheses, only CM drinkers in the negative mood condition reported increased relief expectancies, and only EM drinkers in the positive mood condition reported increased reward expectancies. Theoretical and clinical implications are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)