Combined hyperlipidemia in transgenic mice overexpressing human apolipoprotein Cl

We have generated transgenic mice over-expressing human apolipoprotein CI (apo CI) using the native gene joined to the downstream 154-bp liver-specific enhancer that we defined for apo E. Human apo CI (HuCI)-transgenic mice showed elevation of plasma triglycerides (mg/dl) compared to controls in both the fasted (211 +/- 81 vs 123 +/- 52, P = 0.0001) and fed (265 +/- 105 vs 146 +/- 68, P < 0.0001) states. Unlike the human apo CII (HuCII)- and apo CIII (HuCIII)-transgenic mouse models of hypertriglyceridemia, plasma cholesterol was disproportionately elevated (95 +/- 23 vs 73 +/- 23, P = 0.002, fasted and 90 +/- 24 vs 61 +/- 14, P < 0.0001, fed). Lipoprotein fractionation showed increased VLDL and IDL + LDL with an increased cholesterol/triglyceride ratio (0.114 vs 0.065, P = 0.02, in VLDL). The VLDL apo E/apo B ratio was decreased 3.4-fold (P = 0.05) and apo CII and apo CIII decreased in proportion to apo E. Triglyceride and apo B production rates were normal, but clearance rates of VLDL triglycerides and postlipolysis lipoprotein "remnants" were significantly slowed. Plasma apo B was significantly elevated. Unlike HuCII- and HuCIII-transgenic mice, VLDL from HuCI transgenic mice bound heparin-Sepharose, a model for cell-surface glycosaminoglycans, normally. In summary, apo CI overexpression is associated with decreased particulate uptake of apo B-containing lipoproteins, leading to increased levels of several potentially atherogenic species, including cholesterol-enriched VLDL, IDL, and LDL.     

Performance analysis of available bandwidth estimation tools for grid networks

Modern large-scale grid computing systems for processing advanced science and engineering applications rely on geographically distributed clusters. In such highly distributed environments, estimating the available bandwidth between clusters is a key issue for efficient task scheduling. We analyze the performance of two well known available bandwidth estimation tools, pathload and abget, with the aim of using them in grid environments. Differently than previous investigations (Jain et al., ; Shriram et al., in Passive and active network measurement: 6th international workshop, PAM 2005. Springer, Berlin, 2005), our experiments consider a series of relevant metrics such as accuracy of the estimation, convergence time, degree of intrusion in the grid links, and ability to handle multiple simultaneous estimations. No previous work has analyzed the use of available bandwidth tools for the derivation of efficient grid scheduling.     

Temporal alignment of tissue and arterial data and selection of integration start times for the H(2)(15)O autoradiographic CBF model in PET

A technique has been developed and tested that provides an automated method of temporally aligning the PET tissue activity curve with the arterial activity curve for quantification of cerebral blood flow using the H(2)(15)O autoradiographic model. This technique not only determines the relative time delay between the two curves, but also provides the start time of integration. Variability in computing global cerebral blood flow using this technique is shown to be less than that obtained by trained observers manually selecting parameters and at least as good as that obtained by using another automated alignment technique.     

Grounding power systems above 600 V

The multigrounded neutral system for power systems above 600 V is a reasonable and safe design. The multigrounded neutral system provides 1) safety, 2) low zero sequence impedance than the single-point grounded neutral system, 3) lightning-arrester sizes can be optimized, 4) freezing and arctic conditions have an adverse impact on the zero sequence impedance, 5) cost of equipment for the multigrounded system is lower.     

Friction model for friction stir welding process simulation: Calibrations from welding experiments

The accurate 3D finite element simulation of the Friction Stir Welding (FSW) process requires a proper knowledge of both material and interface behaviors, but friction, the key phenomenon of this process, is quite difficult to model and identify. According to the extreme encountered conditions and the highly coupled nature of the material flow, simple tribological tests are not representative enough, so the welding process itself has been utilized in most analyses of the literature, although its complexity has led to use simplified numerical models and approaches. The recent development of more accurate 3D simulation software, which allows modeling the entire complexity of the FSW process, makes it possible to follow a much more rigorous inverse analysis (or calibration) approach. FSW trials are conducted on an Al 6061 aluminum plate with an unthreaded concave tool. Forces and tool temperatures are accurately recorded at steady welding state, for different welding speeds. The numerical simulations are based on an Arbitrary Lagrangian Eulerian (ALE) formulation that has been implemented in the Forge3^® F.E. software. The main feature of the numerical approach is to accurately compute the contact and frictional surface between the plate and the tool. A first study using Norton's friction model show the great sensitivity of welding forces and tool temperatures to friction coefficients, the need to take into account the changes brought to the contact surface by slight friction variations (thanks to the ALE formulation), the possibility to get very accurate calibrations on forces, and the impossibility to properly render the tool temperature profile. On the other hand, the use of Coulomb's friction model allows obtaining realistic temperature profiles and so calibrating a friction coefficient that offers an excellent agreement with experiments, on forces as much as on tool temperatures, for various welding speeds.     

Use of a microwave cavity for sensing dielectric properties ofarbitrarily shaped biological objects

A rectangular waveguide resonator operating in the H₁₀₅ mode at 3.2 GHz is used in determining the change in resonant frequency, ΔF, and the Q factor of the cavity, ΔT , when measured with and without single corn kernels of various shapes and dimensions. By measuring those variables for a kernel oriented in two positions differing by a 90° rotation with respect to the maximum E-field vector, the average values of ΔF and ΔT are found to be independent of shape. The ratio ΔF/ΔT is independent of size and is a function of the material properties (ε'-1)/ε". This function is shown to be related to the material density, moisture content, or other characteristics when all other properties except the one selected remain unchanged