A novel coronavirus of zoonotic origin(SARSCoV-2)has recently been recognized in patients with acute respiratory disease.COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses.The drastic increase in the number of coronavirus and its genome sequence have given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses.Clinical tests like PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients.However,these techniques are expensive and not readily available for point-of-care(POC)applications.Currently,lack of any rapid,available,and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem.To solve the negative features of clinical investigation,we provide a brief introduction of the general features of coronaviruses and describe various amplification assays,sensing,biosensing,immunosensing,and aptasensing for the determination of various groups of coronaviruses applied as a template for the detection of SARS-CoV-2.All sensing and biosensing techniques developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus,i.e.,SARS-CoV-2.Also,the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system to detect the virus at the early stage of infection to tranquilize the speed and vastity of spreading.Among other approaches investigated among molecular approaches and PCR or recognition of viral diseases,LAMP-based methods and LFAs are of great importance for their numerous benefits,which can be helpful to design a universal platform for detection of future emerging pathogenic viruses. 相似文献
The effect of a yoghurt supplement containing Bifidobacterium pseudocatenulatum G4 or Bifidobacterium longum BB536 on plasma lipids, lipid peroxidation and the faecal excretion of bile acids was examined in rats fed a cholesterol-enriched diet. After 8 weeks, the rats in the positive control (PC) group who were fed the cholesterol-enriched diet showed significant increases in plasma total cholesterol (TC), low-density lipoprotein (LDL) cholesterol, and malondialdehyde (MDA). However, groups fed a cholesterol-enriched diet supplemented with yoghurt containing B. pseudocatenulatum G4 or B. longum BB536 had significantly lower plasma TC, LDL-C, very-low-density lipoprotein (VLDL) cholesterol, and MDA than had the PC group after 8 weeks of treatment. In addition, faecal excretion of bile acids was markedly increased in the rats fed the yoghurt containing B. pseudocatenulatum G4 or B. longum BB536 as compared to the PC and NC groups. 相似文献
Journal of Inorganic and Organometallic Polymers and Materials - Historically, the recording of information over paper has always been regarded as the most important factor for maintaining records... 相似文献
In this study, copper foam was used as a porous medium in place of traditional aluminum fins. A comparison between the two heat exchangers—one with fins and the other with copper foam—was conducted under various conditions. The air inlet velocity ranged from 0.9 to 9.3 m/s, and the water inlet temperature ranged from 10°C to 18°C. Different water flow rates were tested. A comparison was made between the performance of copper foam and aluminum fins by calculating several parameters, including thermal resistance, heat exchanger effectiveness, Colburn factor, Nusselt number, friction factor, and area goodness factor (AG). The experimental results showed that at low air velocities, the heat transfer coefficient for both types of heat exchangers was almost equal. However, at high air velocities, the copper foam exhibited a higher heat transfer coefficient. The Colburn factor was higher for the heat exchanger with copper foam than in the conventional fins, where it was equal to 0.1959 for the copper foam and 0.1186 for the fins. On the other hand, the AG was higher in the case of fins than in the heat exchanger with copper foam. 相似文献
This paper proposes and optimizes a two-term cost function consisting of a sparseness term and a generalized v-fold cross-validation term by a new adaptive particle swarm optimization (APSO). APSO updates its parameters adaptively based on a dynamic feedback from the success rate of the each particle’s personal best. Since the proposed cost function is based on the choosing fewer numbers of support vectors, the complexity of SVM model is decreased while the accuracy remains in an acceptable range. Therefore, the testing time decreases and makes SVM more applicable for practical applications in real data sets. A comparative study on data sets of UCI database is performed between the proposed cost function and conventional cost function to demonstrate the effectiveness of the proposed cost function.
Multidimensional Systems and Signal Processing - Development of medical image segmentation techniques has become one of the most important challenges in many applications that employ... 相似文献
This paper presents a study of the propagation and mitigation of ground vibrations induced by high speed railways using 8 centrifuge tests. In the reported tests here, geofoam is used as a barrier in various locations and arrangements (single and double) to mitigate ground vibrations. The results show that the surface waves guide the propagation pattern of ground vibrations induced by high speed railways and also reveal that geofoam is a proper material for the mitigation of such ground vibrations. While the use of single geofoam barriers can reduce ground vibrations by up to 54.5%, their performance at low input frequencies are undesirable. Double geofoam barriers are used and tested in various locations to eliminate such inconvenient effects and improve the mitigation of ground vibrations. The results show that double geofoam barriers can mitigate the vibrations by about 14%–35% more than a single geofoam barrier and undesirable performances for the mentioned low input frequencies are also eliminated. 相似文献
The granule-bound starch synthase I (GBSSI) encoded by the waxy gene is responsible for amylose synthesis in the endosperm of wheat grains. In the present study, a novel Wx-B1 null mutant line, M3-415, was identified from an ethyl methanesulfonate-mutagenized population of Chinese tetraploid wheat landrace Jianyangailanmai (LM47). The gene sequence indicated that the mutated Wx-B1 encoded a complete protein; this protein was incompatible with the protein profile obtained using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, which showed the lack of Wx-B1 protein in the mutant line. The prediction of the protein structure showed an amino acid substitution (G470D) at the edge of the ADPG binding pocket, which might affect the binding of Wx-B1 to starch granules. Site-directed mutagenesis was further performed to artificially change the amino acid at the sequence position 469 from alanine (A) to threonine (T) (A469T) downstream of the mutated site in M3-415. Our results indicated that a single amino acid mutation in Wx-B1 reduces its activity by impairing its starch-binding capacity. The present study is the first to report the novel mechanism underlying Wx-1 deletion in wheat; moreover, it provided new insights into the inactivation of the waxy gene and revealed that fine regulation of wheat amylose content is possible by modifying the GBSSI activity. 相似文献