K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose

The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10–17 µm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections.     

Biological evaluation and quantitative analysis of antioxidant compounds in pulps of the Amazonian fruits bacuri (Platonia insignis Mart.), ingá (Inga edulis Mart.), and uchi (Sacoglottis uchi Huber) by UHPLC‐ESI‐MS/MS

Bacuri, ingá, and uchi are Amazon fruits consumed specially in the North region of Brazil. Due to their large consumption and the lack of knowledge regarding its chemical composition, these fruits were studied in relation to their Antioxidant chemical constitution. The total phenolic content ranged from 3.86 ± 0.47 to 33.38 ± 1.51 mg GAE/100 g, and the total flavonoid content ranged from 1.75 ± 0.22 to 19.44 ± 0.87 mg QUERE/100 g, where the contents showed a significant correlation with DPPH and ABTS antioxidant assays. Thus, UHPLC‐MS/MS was applied do quantify selected compounds, been citric acid the most abundant for all fruits. Furthermore, samples were screened for their α‐glycosidase and lipase inhibitory effects, in addition to their antimicrobial potentials. Bacuri showed the highest antioxidant and α‐glycosidase inhibitory capacity (IC₅₀ 15.20 μg/mL), whereas uchi and its main metabolite bergenin displayed moderate antimicrobial activities. The results shed light into the potentials of Amazonian fruit sources.

Practical applications

Plant phenolics are essential components of functional foods, due to their antioxidant and enzyme inhibition activities, which are directly linked to several diseases prevention. This is the first study about the quantification of antioxidant compounds in the Amazonian fruits: bacuri, ingá, and uchi. Although they are quite consumed in the North region of Brazil, there are no bio‐products made from them. This study aimed to elucidate the knowledge about the chemical composition and potentialities within these fruits with the practical purpose of highlighting them to future commercial applications. In addition, once we indicate their capabilities, we contribute with local populations in respect to the production of such fruits, which still is a family activity.     

Effect of air temperature on drying kinetics and quality characteristics of osmo-treated jumbo squid (Dosidicus gigas)

The present study investigated the influence of air temperature on the drying kinetics, color, rehydration, total volatile basic nitrogen (TVBN), antioxidant capacity and texture of osmosed jumbo squid fillets during convective dehydration at 50, 60, 70, 80 and 90 °C. The Logarithmic and Two-term models could be used to describe the squid experimental drying curves. Discoloration of product was more noticeable at high drying temperatures where combined effects of non-enzymatic browning as well as protein denaturation modified the original color of the squid samples. Rehydration indexes showed a decrease while texture presented an increase with increasing air-drying temperature probably due to changes in food protein matrix. Total volatile basic nitrogen increased with process temperature. Antioxidant activity presented a decrease with temperature, especially at high drying temperatures. Results of this study demonstrated that the drying kinetics together with the reported quality attributes of the dried squid can be used to improve the final characteristics of the product.     

Production of butanol (a biofuel) from agricultural residues: Part II – Use of corn stover and switchgrass hydrolysates

Acetone butanol ethanol (ABE) was produced from hydrolysed corn stover and switchgrass using Clostridium beijerinckii P260. A control experiment using glucose resulted in the production of 21.06 g L^?1 total ABE. In this experiment an ABE yield and productivity of 0.41 and 0.31 g L^?1 h^?1 was achieved, respectively. Fermentation of untreated corn stover hydrolysate (CSH) exhibited no growth and no ABE production; however, upon dilution with water (two fold) and wheat straw hydrolysate (WSH, ratio 1:1), 16.00 and 18.04 g L^?1 ABE was produced, respectively. These experiments resulted in ABE productivity of 0.17–0.21 g L^?1 h^?1. Inhibitors present in CSH were removed by treating the hydrolysate with Ca(OH)₂ (overliming). The culture was able to produce 26.27 g L^?1 ABE after inhibitor removal. Untreated switchgrass hydrolysate (SGH) was poorly fermented and the culture did not produce more than 1.48 g L^?1 ABE which was improved to 14.61 g L^?1. It is suggested that biomass pretreatment methods that do not generate inhibitors be investigated. Alternately, cultures resistant to inhibitors and able to produce butanol at high concentrations may be another approach to improve the current process.     

STRAIN RATE RELAXATION EFFECT ON FREEZING FRONT GROWTH INSTABILITY DURING PLANAR SOLIDIFICATION OF PURE METALS,PART 1. UNCOUPLED THEORY

Previous models of thermomechanically induced freezing front growth instability have assumed that the casting accumulates elastic strains as it solidifies. While this assumption is useful in providing insight into solidification thermomechanics, it fails to account for inelastic strains that normally accompany elevated temperature deformations. In this paper, growth instability during solidification of a pure metal is reexamined, assuming that the strain rate within the solidifying shell is the sum of elastic, thermal, and viscous components. This requires that a theoretical framework for plane strain thermoviscoelasticity be developed for a solidifying metal. The viscous component leads to strain rate relaxation within the casting and subsequently influences the evolution of the contact pressure and macromorphology of the freezing front. We define a strain rate relaxation parameter that determines the extent to which the casting deforms due to viscous creep. Both short-time and long-time solutions for the contact pressure are developed and subsequently examined for selected values of the strain rate relaxation parameter. The thermal and mechanical fields are assumed to be uncoupled along the metal /mold interface in the present paper while they are coupled along this interface in the companion paper.     

Analysis of nanostructured porous films by measurement of adsorption isotherms with optical fiber and ellipsometry

An optical method to determine the nanostructure and the morphology of porous thin films is presented. This procedure is based on the response of a side-polished optical fiber with the film under study, when an adsorption-desorption cycle is carried out. Spectroscopic ellipsometry provides additional information about the optical properties and adsorption behavior of the film. Pore size distribution, anisotropy, and inhomogeneity of films can be determined by use of these two complementary techniques. To check the performances and suitability of the optical method, we have characterized a typical porous material: a TiO2 film deposited by evaporation. Water vapor has been used for the adsorption cycles. The well-known columnar structure of the evaporated TiO2 has been evidenced, and the relation between the nanostructure and the optical properties of the film is showed.     

Static response in disk packings

1We present experimental and numerical results for displacement response functions in packings of rigid frictional disks under gravity. The central disk on the bottom layer is shifted upwards by a small amount, and the motions of disks above it define the displacement response. Disk motions are measured with the help of a still digital camera. The responses so measured provide information on the force-force response, that is, the excess force at the bottom produced by a small overload in the bulk. We find that, in experiments, the vertical-force response shows a Gaussian-like shape, broadening roughly as the square root of distance, as predicted by diffusive theories for stress propagation in granulates. However, the diffusion coefficient obtained from a fit of the response width is ten times larger than predicted by such theories. Moreover we notice that our data is compatible with a crossover to linear broadening at large scales. In numerical simulations on similar systems (but without friction), on the other hand, a double-peaked response is found, indicating wave-like propagation of stresses. We discuss the main reasons for the different behaviors of experimental and model systems, and compare our findings with previous works.     

Interspecific Differences in Aethia Spp. Auklet Odorants and Evidence for Chemical Defense Against Ectoparasites

The true auklets (Genus Aethia) are small planktivorous seabirds of the Bering Sea and North Pacific. Two species, the crested and whiskered auklets produce volatile citrus-like odorants. We here show that the whiskered auklet odorant is composed predominantly of two odd-numbered aldehydes (heptanal and nonanal) with no detectable unsaturated aldehydes. By comparison the crested auklet odorant is dominated by even-numbered aldehydes, both saturated and monounsaturated, ranging in size from 6 to 12 carbons. This is evidence of species-specific acquisition or biosynthetic pathways. We clarify the chemistry of the crested auklet odorant. We cite evidence that the C-12:1 aldehyde in crested auklets is actually two isomers, (Z)-4-dodecenal and (Z)-6-dodecenal. We also report on experimental evidence that aldehyde constituents kill and repel ectoparasites. Efficacy of the aldehydes may increase when they are combined in a mixture. The repellency of the mixture increases with chemical concentration. This suggests that individuals with higher chemical production are likely to repel ectoparasites more effectively.