Natural T cells in the human liver: cytotoxic lymphocytes with dual T cell and natural killer cell phenotype and function are phenotypically heterogenous and include Valpha24-JalphaQ and gammadelta T cell receptor bearing cells

The adult liver contains lymphocytes with a unique phenotypic distribution compared to blood and other organs. We have characterized a human lymphocyte population that exhibits dual T cell and natural killer (NK) cell phenotype and function, denoted natural T (NT) cells, in nine normal adult liver specimens. Flow cytometry revealed that up to 55% (mean 27%) of hepatic (but <6% of peripheral) CD3+ lymphocytes expressed CD56, CD161 and/or one or more of the killer inhibitory receptors (KIR) p58.1, p58.2, p70 and CD94. NK function was attributed to the CD3+CD56+ cells by the demonstration that hepatic, but not peripheral, CD3+ lymphocytes could be induced to lyse NK-sensitive K562 target cells, while CD56- cells from both compartments could not. Three color flow cytometric analysis of fresh hepatic cells indicated that CD3+CD56+ NT cells can be either CD8+, CD4+ or CD4 CD8-, they express alphabeta or gammadelta T cell receptors (TCR) and CD161 and KIRs, but rarely CD16. Hepatic NT cells predominantly express the mature/activated CD45RO and CD56dim phenotypes. Analysis of mRNA production by isolated NT cells indicated a preferential usage of the invariant CD1-restricted Valpha24-JalphaQ TCR. The presence of such large numbers of chronically activated NT cells provides compelling evidence that the liver has unique immunoregulatory functions.     

A theoretical analysis of optical clock extraction using aself-pulsating laser diode

The potential for using inexpensive compact disc laser diodes as optical clock extraction elements in transparent networks has led to an increase in research into the dynamics of self-pulsating laser diodes. We use a rate-equation model to simulate the synchronization of the self-pulsating laser output pulses to a periodic optical signal, In particular, we investigate the time it takes for the laser to synchronize to the input signal and also, the time taken for the laser to unlock when the signal is removed. The effect of varying the power of the optical signal and the detuning of the input signal frequency relative to the laser's self-pulsation frequency are determined. Our results enable us to identify important issues which need to be addressed when a self-pulsating laser diode is used in a clock extraction subsystem, In particular, we find that the signal frequency and laser free-running frequency must be as close as possibility to minimize errors. Also, the higher the signal power the quicker the laser synchronizes to the signal, although we find that if the power becomes too large the laser can no longer lock, which would cause a significant increase in detection errors     

Graphing the order of the sexes: Constructing, recalling, interpreting, and putting the self in gender difference graphs.

Graphs seem to connote facts more than words or tables do. Consequently, they seem unlikely places to spot implicit sexism at work. Yet, in 6 studies (N = 741), women and men constructed (Study 1) and recalled (Study 2) gender difference graphs with men’s data first, and graphed powerful groups (Study 3) and individuals (Study 4) ahead of weaker ones. Participants who interpreted graph order as evidence of author “bias” inferred that the author graphed his or her own gender group first (Study 5). Women’s, but not men’s, preferences to graph men first were mitigated when participants graphed a difference between themselves and an opposite-sex friend prior to graphing gender differences (Study 6). Graph production and comprehension are affected by beliefs and suppositions about the groups represented in graphs to a greater degree than cognitive models of graph comprehension or realist models of scientific thinking have yet acknowledged. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two Ehrlichia canis strains

These is increasing evidence to suggest that central noradrenergic mechanisms may contribute to the central nervous system manifestations of acute liver failure. To further elucidate this possibility, extracellular brain concentrations of the monoamines, noradrenaline (NA), dopamine (DA), and serotonin, were measured by high-performance liquid chromatography with electrochemical detection in microdialysates from the extracellular compartment of frontal cortex in rats with acute (ischemic) liver failure at various times during the progression of encephalopathy and brain edema, as well as in obligate control groups of animals. In addition, binding sites for the noradrenergic receptor subtype ligands, [3H]-prazosin (alpha1 sites), [3H]-RX821002 (alpha2 sites), and [125]I-iodopindolol (beta sites), were assessed using quantitative receptor autoradiography in regions of the brains of rats at coma stage of acute liver failure and of control groups of animals. Coma stages of encephalopathy in acute liver failure were associated with selectively increased noradrenaline concentrations (P < .05) and a concomitant selective loss of alpha1 and beta1 sites in frontal cortex and thalamus. These findings add to a growing body of evidence that central noradrenergic function is modified in acute liver failure and suggest that alpha1/beta1 receptor-mediated noradrenergic mechanisms may play a role in the pathogenesis of brain edema and encephalopathy in this condition.     

Cognitive Status, Information Structure, and Pronominal Reference to Clausally Introduced Entities

This paper investigates reference to clausally introduced entities and proposes an explanation for why these are more readily available to immediate subsequent reference with a demonstrative pronoun than with the personal pronoun,it. New evidence is provided supporting proposals that such entities are typically activated, but not brought into focus, upon their introduction into a discourse. The study also provides further insight into the role of information structure, lexical semantics, presuppositional contexts, and syntactic structure in bringing an entity into focus of attention.     

Orientation specificity and spatial updating of memories for layouts.

This article examines the degree to which knowledge about the body's orientation affects transformations in spatial memory and whether memories are accessed with a preferred orientation. Participants learned large paths from a single viewpoint and were later asked to make judgments of relative directions from imagined positions on the path. Experiments 1 and 2 contribute to the emerging consensus that memories for large layouts are orientation specific, suggesting that prior findings to the contrary may not have fully accounted for latencies. Experiments 2 and 3 show that knowledge of one's orientation can create a preferred direction in spatial memory that is different from the learned orientation. Results further suggest that spatial updating may not be as automatic as previously thought. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The use of a surface adhesion immunofluorescent (SAIF) method for the rapid detection of Yersinia enterocolitica serotype O:3 in meat

Defective trafficking of the cystic fibrosis transmembrane conductance regulator (CFTR) is the most common cause of cystic fibrosis. In chloride-secreting epithelia, it is well established that CFTR localizes to intracellular organelles and to apical membranes. However, it is controversial whether secretagogues regulate the trafficking of CFTR. To investigate whether acute hormonal stimulation of chloride secretion is coupled to the trafficking of CFTR, we used the intact shark rectal gland, a model tissue in which salt secretion is dynamically regulated and both chloride secretion and cellular CFTR immunofluorescence can be quantified in parallel. In rectal glands perfused under basal conditions without secretagogues, Cl- secretion was 151+/-65 microeq/h/g. Vasoactive intestinal peptide (VIP), forskolin, and genistein led to 10-, 6-, and 4-fold increases in Cl- secretion. In basal glands, quantitative confocal microscopy revealed CFTR immunofluorescence extending from the apical membrane deeply into the cell (7.28+/-0.35 micron). During stimulation with secretagogues, apical extension of CFTR immunofluorescence into the cell was reduced significantly to 3.24+/-0.08 micron by VIP, 4.08+/-0.13 by forskolin, and 3.19+/-0.1 by genistein (P < 0.001). Moreover, the peak intensity of CFTR fluorescence shifted towards the apical membrane (peak fluorescence 2.5+/-0.13 micron basal vs. 1.51+/-0.06, 1.77+/-0.1, and 1.38+/-0.05 for VIP, forskolin, and genistein; all P < 0.001). The increase in both Cl- secretion and apical CFTR trafficking reversed to basal values after removal of VIP. These data provide the first quantitative morphological evidence for acute hormonal regulation of CFTR trafficking in an intact epithelial tissue.     

Methane and carbon dioxide sensing using a DFB laser diode operating at 1.64 mu m

Selective gas sensing of CH/sub 4/ and CO/sub 2/ using a single tunable 1.64 mu m DFB laser diode is demonstrated for the first time. By precise wavelength tuning of the diode to non-coincident absorptions of the two gases in their overlapping spectral region, one can detect both using absorption spectroscopy.<>     

Electron Backscatter Diffraction as a Domain Analysis Technique in BiFeO/sub 3/-PbTiO/sub 3/Single Crystals

xBiFeO₃-(1-x)PbTiO₃ single crystals were grown via a flux method for a range of compositions. Presented here is a study of the domain configuration in the 0.5BiFeO₃-0.5PbTiO₃ composition using electron backscatter diffraction to demonstrate the ability of the technique to map ferroelastic domain structures at the micron and submicron scale. The micron-scale domains exhibit an angle of approximately 85deg between each variant, indicative of a ferroelastic domain wall in a tetragonal system with a spontaneous strain, c/a - 1 of 0.10, in excellent agreement with the lattice parameters derived from X-ray diffraction. Contrast seen in forescatter images is attributed to variations in the direction of the electrical polarization vector, providing images of ferroelectric domain patterns.     

Thinking about the weather: How display salience and knowledge affect performance in a graphic inference task.

Three experiments examined how bottom-up and top-down processes interact when people view and make inferences from complex visual displays (weather maps). Bottom-up effects of display design were investigated by manipulating the relative visual salience of task-relevant and task-irrelevant information across different maps. Top-down effects of domain knowledge were investigated by examining performance and eye fixations before and after participants learned relevant meteorological principles. Map design and knowledge interacted such that salience had no effect on performance before participants learned the meteorological principles; however, after learning, participants were more accurate if they viewed maps that made task-relevant information more visually salient. Effects of display design on task performance were somewhat dissociated from effects of display design on eye fixations. The results support a model in which eye fixations are directed primarily by top-down factors (task and domain knowledge). They suggest that good display design facilitates performance not just by guiding where viewers look in a complex display but also by facilitating processing of the visual features that represent task-relevant information at a given display location. (PsycINFO Database Record (c) 2010 APA, all rights reserved)