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W Cao M Britos-Bray DF Claxton CA Kelley NA Speck PP Liu AD Friedman 《Canadian Metallurgical Quarterly》1997,15(11):1315-1327
CBF beta-SMMHC is expressed from the inv(16) chromosome in M4Eo AML. Mice lacking CBF subunits or expressing the CBF beta-SMMHC or AML1-ETO oncoproteins failed to develop definitive hematopoiesis. To investigate these effects on hematopoiesis, we expressed CBF beta-SMMHC from the metallothionein promoter, in both 32D cl3 myeloid cells and Ba/F3 B-lymphoid cells. Addition of zinc increased CBF beta-SMMHC levels more than tenfold, with higher levels evident in Ba/F3 lines. Levels obtained in 32D cl3 cells were similar to those of endogenous CBF beta. Indirect immunofluorescence revealed zinc-inducible speckled, nuclear staining in Ba/F3 cells and diffuse nuclear staining in 32D cl3 cells. CBF beta-SMMHC reduced endogenous CBF DNA-binding fivefold in both cell types, increased cell generation time 1.9-fold, on average, in 32D cl3 cells and 1.5-fold in Ba/ F3 cells and decreased tritiated thymidine incorporation into DNA correspondingly. CBF beta-SMMHC increased the proportion of cells in G1 1.7-fold, on average, in 32D cl3 and Ba/F3 cells, and decreased the proportion of cells in S phase by a similar degree. CBF beta-SMMHC induced a marked increase in hypophosphorylated Rb, but did not alter IL-3 Receptor alpha or beta subunit levels. Neither apoptosis nor 32D differentiation was induced by zinc in IL-3 in these lines. Induction of CBF beta-SMMHC in 32D cl3 cells did not inhibit their differentiation to neutrophils or their expression of myeloperoxidase mRNA in G-CSF, and did not produce an eosinophilic phenotype. Additional, proliferative genetic changes in M4eo AMLs might potentiate inhibition of differentiation by CBF beta-SMMHC by allowing its increased expression. 相似文献
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Economic ideas and processes are becoming increasingly integrated with more traditional engineering and hydrologic models
of water management problems. Combining economic management concepts and performance indicators with an engineering-level
of understanding of a hydrologic system can provide results and insights more directly relevant for water management decisions
and policies. When such models are developed and used with involvement of stakeholders, they can become a basis for shared
understanding of water problems as a foundation for negotiated management and policy solutions. When implemented with optimization
software, integrated hydro-economic models also can suggest promising innovative solutions for policy-makers to consider.
Their applications to river basin management problems are reviewed. Economic and integrated economic-engineering-hydrologic
modeling is then discussed in the context of the evolving European Water Framework Directive. Relevant items are cost recovery
and water pricing, cost-effectiveness of water management measures, and public participation in decision processes. 相似文献
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Improving the vacuum would increase the insulation capacity of commercially available glass containers by a factor of about two. To do this, pressures of 10?4 mbar must be attained. Different approaches were taken to meet this objective. As an alternative to today's commonly practiced sealing method, three possibilities are introduced which eliminate the necessity of pump stub narrowing which causes reduced conductance. Furthermore, depending on the sealing process used, the gases occuring during sealing are either avoided completly or they can at least be reduced by having the chance of exact temperature control resp. by a lower separation temperature. A completly different approach was taken by using Getters, of which a metal hydride on a TiVMn base proved especially suitable. It is activated by heat and can be integrated into the normal production process without any problem. Aside from a shorter pump period, the added advantage here is the containment of the gasses occuring during sealing as well as that of the longterm gasses. 相似文献
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Karl Heinz Schwalbe 《Engineering Fracture Mechanics》1993,45(6):751-758
The Engineering Treatment Model (ETM) provides a means for estimating the load line displacement of a cracked body. Under contained yielding conditions the model uses available linear elastic solutions with an Irwin-type plasticity correction of the crack length. The magnification factor (F/Fy)1/n describing the material's strain hardening behaviour is applied for extrapolation into the regime of net section yielding. Comparison of ETM estimates with finite element and experimental results shows that the assumptions of the model are realistic. 相似文献
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Lázaro Valentin Zuquette Janaina Barrios Palma Osni José Pejon 《Bulletin of Engineering Geology and the Environment》2005,64(3):257-271
There are more than 20,000 uncontrolled sanitary landfill areas in Brazil. The paper reports an integrated study undertaken
to develop an environmental assessment of the uncontrolled sanitary landfill area of the city of Po?os de Caldas, State of
Minas Gerais, Brazil. A number of remedial alternatives were evaluated. The USGS modular 3D finite difference groundwater
flow model (Modflow) and Modular 3D Finite Difference Mass Transport Model (MT3D) software were used to simulate groundwater
flow and contaminant transport modelling, as well as to predict changes due to the proposed remediation measures. The remedial
alternatives considered for normal conditions from 20 to 40 year periods were waste removal, vertical cut-off barriers, extraction
wells and top capping. The results indicated that extraction wells and waste removal are the best alternatives because they
significantly reduce both the extension and levels of the contamination plume even after 5 years. The extraction well proved
a less expensive remedial alternative than waste removal.
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