Molecular Identification of the G-Protein Genes and Their Expression Profiles in Response to Nitrogen Deprivation in Brassica napus

Heterotrimeric guanine nucleotide binding protein (G-protein) consisting of Gα, Gβ, and Gγ subunits is one of the key signal transducers in plants. Recent studies indicated that G-protein has been proposed as an important mediator of nitrogen responses in rice, wheat, and Arabidopsis. However, little is known about these G-proteins in Brassica napus (B. napus), except for three identified G-proteins, BnGA1, BnGB1, and BnGG2. Therefore, the aim of the present study is to characterize the members of the G-protein gene family in allotetraploid B. napus and to analyze their expression profiles in response to nitrogen deprivation. In total, 21 G-protein family members were identified in B. napus, encoding two Gα, six Gβ, and 13 Gγ. Sequence and phylogenetic analyses showed that although genome-wide triploid events increased the number of genes encoding Gα, Gβ, and Gγ subunits, the gene structure and protein properties of the genes encoding each G-protein subunit were extremely conserved. Collinearity analysis showed that most G-protein genes in B. napus had syntenic relationships with G-protein members of Arabidopsis, Brassica rape (B. rapa), and Brassica oleracea (B. oleracea). Expression profile analysis indicated that Gα and C-type Gγ genes (except BnGG10 and BnGG12 were highly expressed in flower and ovule) were barely expressed in most organs, whereas most Gβ and A-type Gγ genes tended to be highly expressed in most organs. G-protein genes also showed various expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, Gα and five C-type Gγ genes were upregulated initially in roots, while in leaves, Gα was downregulated initially and five C-type Gγ genes were highly expressed in different times. These results provide a complex genetic dissection of G-protein genes in B. napus, and insight into the biological functions of G-protein genes in response to nitrogen deficiency.     

Research on the stability of nearly zero flattened dispersion of photonic crystal fibers

To analyze the stability of nearly zero flattened dispersion, the dispersion deviations for three kinds of PCFs are calculated when the hole diameters deviate from their designed values. Numerical results show that around the wavelength of 1.55 μm, the dispersion deviations of both the PCF with three-fold symmetry core and the PCF with hexagonal lattice are much less than that of the PCF with different hole diameters in different rings. Therefore, the stabilities of nearly zero flattened dispersion of the first two kinds of PCFs are much better than that of the last one. Considering the confinement loss, the PCF with three-fold symmetry core is preferable to practical use.     

三轴联动数控铣削的刀具干涉判断与避免

针对平底刀、球形刀和环形刀的三轴联动数控铣削自由曲面的不同特点,分析可能产生的干涉现象,提出干涉判 断和干涉避免的算法。     

The Thermal-Chemical Damage in Biological Material under Laser Irradiation

A mathematical model for thermal-chemical damage to biological materials excited by laser irradiation is described. The chemical rate equations for protein denaturization are used to predict radii of damage for cases where the input-laser-energy distribution results in the uniform heating of a small sphere or a disk. These rate equations are limited to a single-hit process. Experimental checks on this model are presented for ruby-laser irradiation of small carbon particles in egg albumin and for CO2?laser surface heating of egg albumin.     

N，N-二乙基羟胺与Np(Ⅵ)氧化还原反应动力学研究

研究了ＮＨＯ３介质中,Ｎ,Ｎ－二乙基羟胺还原Ｎｐ（Ⅵ）的动力学性能和行为。通过考究还原剂浓度和酸度等条件对Ｎｐ（Ⅵ）动力学过程的影响,确定了反应的动力学速率主程为－ｄｃ（Ｎｐ（     

某待建滨海核电厂厂址年均大气扩散因子计算模式与参数的实验研究

本文在某待建滨海核电厂厂趣现场野外实验基础上提出了计算该核电厂年均大气扩散因子的计算模型。此模型既考虑了向岸流与离岸流的差别,又考虑了混合层与热内边界层,混合释放与扩散参数随陆距变化等因素的综合影响。模型中所需要的各类参数,包括在１９９５,１９９６年间在该厂趣野外大气试验中获取的百米气象铁塔４个不同高度上的风,温逐时三维联合频率;湍流测量结合风洞实验获取的离岸流与向岸流的扩散参数。     

Fe_(16)N_2 的沉淀效应

含氮马氏体室温时效和低于１７５℃回火时有Ｆｅ１６Ｎ２沉淀。Ｆｅ１６Ｎ２的沉淀效应显著,于１００℃回火时沉淀效应最大,ＨＶ、ｃ／ａ比和α（２２０）半高宽均达到最大值。Ｆｅ１６Ｎ２淀淀具有很高的强化效果,纯铁和２０钢渗氮后于１００℃回火,硬度分别提高５８％和６２％。Ｆｅ１６Ｎ２的沉淀效应具有广泛的应用前景     

焊管机用Cr12MoV钢挤压辊的补焊修复

介绍了焊管机组用Cr12MoV钢挤压辊的补焊修复原理及堆焊工艺。