利用膨胀床吸附技术单步纯化分子伴侣—GroEL

Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purification of biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an important protein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarified homogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provided a single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and column washing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bed mode in the purification of GroEL from cell disruptate.     

微生物转化法合成L-酪氨酸

以对羟基苯甲醛、海因、氢氧化钠为原料 ,化学合成底物对羟基苯丙酮酸。采用来自大肠杆菌的天冬氨酸转氨酶对对羟基苯丙酮酸进行生物转化 ,并对转化条件进行了初步的研究 ,最终得到L-酪氨酸 ,转化率 75 %。     

A molecular dynamics simulation study on the crystallization of 22,8-polyurethane

By means of the molecular dynamics (MD) simulation, the crystallization mechanism of 22,8-polyurethane which contains hydrogen-bond units is investigated and the results show that the crystallization process at a fixed temperature can be characterized by three stages: (1) The extended chain collapses to a globular random coil; (2) The random coil reorganizes into an ordered lamellar structure; (3) Accompanied with the segments clustering due to the hydrogen-bond formation, the lamellar develops with local defects. Two kinds of hydrogen-bond, which are formed between NH group and CO group (N-H?OC), and between NH group and urethane alkoxy oxygen (N-H?O), respectively, are found to play an important role in the crystallization process of 22,8-polyurethane. Furthermore, the effect of temperature on the crystallization is also studied by selecting three temperatures 200, 300 and 400 K. The lower the crystal temperature is, the slower the crystallization rate is and the stronger the hydrogen-bonding interactions are presented. This is in harmony with the experimental results.     

勃姆石分解制ρ—Al2O3结合剂的研究

研究了以勃姆石为原料快速分解制取ρ－Ａｌ２Ｏ３的方法，探讨了原料在炉中停留时间，最佳分解温度及加料速度，对ρ－Ａｌ２Ｏ３的性能鉴定及耐火浇注料的试验结果表明：这种ρ－Ａｌ２Ｏ３的相组成与用Ａｌ（ＯＨ）３分解所得的ρ－Ａｌ２Ｏ３不同，但同样可用作优质浇注粒的     

加氢法与生物法联合脱除催化柴油有机硫探索实验

由于存在空间位阻,二苯并噻吩(DBT)及其烷基化衍生物(Cx-DBT)中的硫原子很难与加氢催化剂接触.因此,作为柴油脱硫的主要工艺,加氢催化对于柴油中的主要有机硫化物DBT和Cx-DBT的脱除率比较低,难以实现深度脱硫.针对硫质量分数为3 358 μg/g的催化柴油,采用加氢脱硫与生物脱硫相结合的办法进行处理后,催化柴油的硫质量分数降低到20μg/g以下,使用气相色谱-原子发射仪对柴油脱硫前后的硫分布进行检测,发现2种工艺的结合能有效地脱除催化柴油组分中绝大多数的DBT和Cx-DBT,显示出良好的应用前景.     

空心微珠表面磁控溅射和化学镀金属膜的特性比较

通过采用磁控溅射方法和化学镀的方法,对无机空心微珠颗粒表面进行了镀金属膜的研究.采用化学镀方法所得的颗粒表面所镀金属层疏松、不均匀、还有未被金属遮盖的孔洞,金属层与空心微珠表面的附着力小,金属薄膜容易脱落.采用磁控溅射方法所镀的空心微珠表面没有明显的金属颗粒,表面所镀金属致密、均匀,表面覆盖完全,没有孔洞,金属层与空心微珠之间的附着力大,即使采用挤压等方法也没有发现金属层与空心微珠表面分离的现象出现.XRD测试表明,采用两种方法所镀的金属其金属的结构和性质几乎没有区别,可以达到同样的应用效果.另外,化学镀方法流程较复杂,易产生环境污染,但适合于超微细颗粒的镀膜.磁控溅射方法是物理方法,没有环境污染,流程也比较简单,但在处理超微细颗粒时必须采用强有力的分散手段保证每个颗粒均匀镀膜.     

如何撰写肥料发明专利申请文件 第八讲 用废物、废料制造肥料申请文件的撰写

这一讲着重介绍了用废物、废料制造肥料的专利申请文件撰写中的几个问题。     

改性磁性壳聚糖微球的制备、表征及性能研究

以(NH4)2Fe(SO4)2.6H2O、NH4Fe(SO4)2.12H2O和壳聚糖为原料,经羟丙基化、胺基化,采用一步包埋法制备了一种新型的多胺基化磁性壳聚糖微球。通过正交实验法确定了磁性微球的最佳制备条件,即搅拌速度1200r/min,壳聚糖用量3.0g,环氧氯丙烷用量2.5mL,乙二胺用量2.5mL。并用IR、TG、XRD和SEM对其结构及形貌进行了表征。结果表明,Fe3O4磁性粒子已包埋了一层胺基化壳聚糖。磁性微球胺基含量为2.302mmol/g;呈较规则的球形,平均粒径为209nm,且具有顺磁性和良好的耐酸性。     

比重瓶法与密度仪法测定香精密度相关性的回归分析

目前测定香精密度的方法主要有标准传统法-比重瓶法和密度仪法。用这两种方法测得的结果一致与否,为探求这两者之间相关关系的密切程度和表现形式,通过测定系列香精的密度,并对所得数据进行线性相关和回归分析,得出了比重瓶法与密度仪法所得结果的相关方程。利用该方程可以由密度仪法测得的结果计算出比重瓶法的密度,经计算出的结果与标准方法比较一致。通过回归方程,可以用密度仪法代替标准法来测定香精的密度。     

Studies toward the synthesis of the shark repellent pavoninin-5

Sharks are the most dangerous predators of people in the sea, resulting in people being mauled and killed each year. A shark repellent could help to diminish this danger. The aglycone of the shark repellent pavoninin-5, (25R)-cholest-5-en-3β,15α,26-triol (5a), was synthesized from diosgenin (9). Removing mercury from the Clemmensen reduction of 9 gave a higher yield of (25R)-cholest-5-en-3β,16β,26-triol, 10a, and was also more environmentally friendly. Attempted methods for the transposition of the C-16β hydroxyl to the 15α position are described. A successful method for this transposition via the 15α-hydroxy-16-ketone, 8a, using the Barton deoxygenation reaction on the 16-alcohol 14b, is reported.