Primer-induced labeling of pea and field bean chromosomes in situ and in suspension

A protocol for primed in situ DNA labeling (PRINS) was optimized for pea (Pisum sativum L.) and field bean (Vicia faba L.) chromosomes attached to coverslips. Cloned DNA or synthetic oligonucleotides were used as probes for repetitive DNA sequences (rDNA, Fok-element) and different reaction conditions were tested to achieve the highest specific signal-to-background ratio. A procedure based on direct labeling by fluorescein-dUTP was compared with an indirect one using digoxigenin detected by fluorescently labeled antibody. Under optimal conditions, strong and specific signals were obtained exclusively on chromosome regions known to contain respective DNA sequences. Compared to the direct labeling, significantly stronger signals were obtained when the indirect procedure was used. Both types of labeling were successfully applied to chromosomes in suspension and were shown to produce signals comparable to that obtained with chromosomes attached to coverslips. It is expected that primed in situ DNA labeling en suspension (PRINSES) will provide a basis for flow-cytometric discrimination and sorting of otherwise indistinguishable chromosomes according to their specific fluorescent labeling.     

Hierarchical Self‐Assembly of Peptide‐Coated Carbon Nanotubes

Numerous applications, from molecular electronics to super‐strong composites, have been suggested for carbon nanotubes. Despite this promise, difficulty in assembling raw carbon nanotubes into functional structures is a deterrent for applications. In contrast, biological materials have evolved to self‐assemble, and the lessons of their self‐assembly can be applied to synthetic materials such as carbon nanotubes. Here we show that single‐walled carbon nanotubes, coated with a designed amphiphilic peptide, can be assembled into ordered hierarchical structures. This novel methodology offers a new route for controlling the physical properties of nanotube systems at all length scales from the nano‐ to the macroscale. Moreover, this technique is not limited to assembling carbon nanotubes, and could be modified to serve as a general procedure for controllably assembling other nanostructures into functional materials.     

The giant dispersion of critical currents in a superconductor with fractal clusters of a normal phase

The influence of fractal clusters of a normal phase on the dynamics of a magnetic flux trapped in a percolation superconductor is considered. The critical depinning current distribution and the current-voltage characteristics of fractal superconducting structures in the resistive state are determined for an arbitrary fractal dimension of the cluster boundaries. The interval of fractal dimensions is found in which the dispersion of critical currents exhibits unlimited growth. It is established that the fractality of clusters favors a decrease of the electric field arising during the magnetic flux motion, thus increasing the critical current value. The region featuring the giant dispersion of critical currents can be expected to provide for the maximum current-carrying capacity of a superconductor.     

Limiting Hardening Stress Levels in Sheet Glass

The limiting level of sheet glass hardening is presented based on three classification strength criteria and is equal to about 500 MPa.     

Screening and characterization of microorganisms with glutaryl-7ADCA acylase activity

A screening of microorganisms producing glutaryl-7ADCA acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7ADCA), has been carried out in soil samples. Five microorganisms expressing acylase activity were isolated and classified as Bacillus cereus, Achromobacter xylosooxidans, Bacillus sp., Pseudomonas sp. and Pseudomonas paucimobilis. The screening was carried out by preparing enrichment cultures containing glutaryl-7-ADCA or cephalosporin C as the selective carbon source. Four model compounds (adipoyl-, glutamyl- and glutaryl-p-nitroanilide and glutarylcoumarin), mimicking the glutaryl-7ADCA beta-lactam moiety, were synthesized as substrates suitable for the rapid screening of the microorganisms (2500) isolated from the enrichment cultures. A total of 300 strains were active on the model substrates and only 5 displayed acylase activity on glutaryl-7ADCA. The fermentation parameters, such as pH and inducer concentration, for the optimal acylase expression and acylase specificity towards the model substrates were different for each strain.     

Possibility of Using a High-Pressure Pneumatic Pump in the Porcelain Industry

The advantages and disadvantages of using a high-pressure pneumatic pump at porcelain and insulator works are discussed. The testing results of a prototype pump are described.     

Comparison between the Diffusion and Relaxation Models of Longitudinal Dispersion

The properties of the relaxation (wave) model of longitudinal dispersion are considered. The diffusion and relaxation models of longitudinal dispersion are compared. It is shown that, depending on its coefficients, the relaxation model can be assigned to two main classes and one intermediate class provided the boundary conditions for the model equations are correctly formulated. New equations are obtained, whose solutions are asymptotically close to the solutions of the diffusion model.     

Piezoelectric fiber optic phase modulator with a reduced level of spurious polarization modulation

It is experimentally demonstrated that a tenfold decrease in the level of spurious polarization modulation can be achieved in a fiber optic phase modulator comprising two fiber sections featuring the polarization mode conversion, arranged on the same piezoelement.     

A quantum full adder for a scalable nuclear spin quantum computer

We demonstrate a strategy for implementation a quantum full adder in a spin chain quantum computer. As an example, we simulate a quantum full adder in a chain containing 201 spins. Our simulations also demonstrate how one can minimize errors generated by non-resonant effects.