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R Fabian-Fine U H?ger EA Seyfarth IA Meinertzhagen 《Canadian Metallurgical Quarterly》1999,19(1):298-310
The mechanosensory organs of arachnids receive diverse peripheral inputs. Little is known about the origin, distribution, and function of these chemical synapses, which we examined in lyriform slit sense organ VS-3 of the spider Cupiennius salei. The cuticular slits of this organ are each associated with two large bipolar mechanosensory neurons with different adaptation rates. With intracellular recording, we have now been able to correlate directly the staining intensity of a neuron for acetylcholinesterase with its adaptation rate, thus allowing us simply to stain a neuron to identify its functional type. All rapidly adapting neurons stain more heavily than slowly adapting neurons. Immunostaining of whole-mount preparations reveals GABA-like immunoreactive fibers forming numerous varicosities at the surface of all sensory neurons in VS-3; peripheral GABA-like immunoreactive somata are lacking. Sectioning the leg nerve procures rapid degeneration of most fiber profiles, confirming that the fibers are efferent. Punctate synapsin-like immunoreactivity colocalizes to these varicosities, although some synapsin-like immunoreactive puncta are GABA-immunonegative. Fibers with similar immunoreactivities are also associated with trichobothria, tactile hairs, internal joint receptors, i.e. other types of spider mechanosensory organs. In organ VS-3, immunoreactivity is most dense across the initial axon segment. The exact distribution of peripheral synapses was reconstructed from a 10-microm-long electron micrograph series of the dendritic, somatic, and initial axon regions of acetylcholinesterase-stained VS-3 neurons. These reveal a pattern similar to that of the synapsin-like immunoreactivity. Two different types of synapse were distinguished on the basis of their presynaptic vesicle populations. Many peripheral synapses thus appear to derive from efferent GABA-like immunoreactive fibers and probably provide centrifugal inhibitory control of primary mechanosensory activities. 相似文献
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Hydraulic fractures have been created in fine-grained formations at depths of 2–10 m to improve the performance of environmental remediation projects at dozens of locations and in a wide range of geologic conditions. The effectiveness of a hydraulic fracture during remediation will depend primarily on its form; that is, its shape, thickness, orientation, length, width, and location with respect to the borehole. The forms of many hydraulic fractures have been determined by mapping exposures in excavations and by compiling split-spoon sampling data. These observations indicate that a typical hydraulic fracture at shallow depths is gently dipping, slightly elongate in plan, and slightly asymmetric with respect to the parent borehole. Shallow hydraulic fractures lift the ground surface to produce gentle domes, and the pattern of uplift reflects the location and thickness of the fracture at depth. The forms of hydraulic fractures created over a narrow range of depths at the same site are similar, but the forms can vary markedly between sites and at different depths at the same site. This indicates that the forms of hydraulic fractures will be relatively consistent when they are created under similar conditions, but changes in geologic conditions can markedly affect fracture form. 相似文献
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N Sattar IA Greer A Rumley G Stewart J Shepherd CJ Packard GD Lowe 《Canadian Metallurgical Quarterly》1999,81(1):71-75
BACKGROUND: Chronic leukemia is a disease characterized by the malignant proliferation of immunologically incompetent lymphocytes. The knowledge of open heart surgery in patients with this disorder is limited. METHODS: Twelve patients with chronic lymphocytic leukemia underwent open heart surgery (nine coronary artery bypass grafting (CABG), two aortic valve replacement (AVR), one CABG and AVR) from September 1991 to September 1996. There were nine males and three females with a mean age of 68 years (41-81 years). Staging was assigned according to the Rai Classification. There were seven Stage 0, two Stage I, zero Stage II, one Stage III and two Stage IV patients. Cardiopulmonary bypass (CPB) was performed using standard techniques of cannulation, moderate hypothermia and antegrade/retrograde cardioplegia. RESULTS: Hospital mortality occurred in two (17%) patients. Both patients died of sepsis. Hospital morbidity occurred in seven (58%) patients. The most common complications were infections. Five patients were found to have other malignancies (basal cell, laryngeal, prostate, bladder and breast cancers). Transfusion of blood products was required in eight (67%) patients. The average length of stay was 15 days (7-50 days). Follow-up was complete. Late mortality occurred in four patients at a mean of 7 months (1-18 months). All deaths were non-cardiac related (ruptured AAA, kidney failure, respiratory failure and sepsis). Six patients remain alive at a mean of 25 months (1-48 months). CONCLUSION: Hospital mortality and morbidity in patients with chronic lymphocytic leukemia undergoing open heart surgery are high. Infection is the leading cause of hospital death, as well as the most common complication. The majority of patients receive blood products during the course of their hospitalization. Late mortality is high and non-cardiac related. Based on these findings, a re-definition of the aims, goals and expectations of open heart surgery in patients with chronic leukemia is necessary. Suggestions in management are presented. 相似文献
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IA Kostanyan EV Navolotskaya RI Nurieva VP Zav'ialov VM Lipkin 《Canadian Metallurgical Quarterly》1997,23(10):805-808
A specific interaction of [3H]Glu with T lymphocytes from the blood of healthy donors (Kd = 0.236 microM) was revealed and described. It was found that unlabeled quisqualate, a structural analogue of L-glutamic acid, and unlabeled dipeptides Ala-Glu, Glu-Ala, and Glu-Glu competitively inhibit the specific binding of [3H]Glu to T lymphocytes (with Ki 0.19, 2.4, 3.4, and 1.2 microM, respectively). Binding experiments with conjugates of labeled and unlabeled glutamic acid with dextran showed that the receptors of [3H]Glu are localized on the outer surface of the plasma membrane of T lymphocytes. 相似文献
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DP Murdoch DG Armstrong JB Dacus TJ Laughlin CB Morgan LA Lavery 《Canadian Metallurgical Quarterly》1997,36(3):204-8; discussion 256
The purpose of this study is to report the prevalence of reamputation following resection of the great toe and first ray in adults with diabetes. We abstracted the medical records of 90 diabetic great-toe and first-ray amputees admitted between 1981 and 1991. The most common etiologies of initial amputations were ulcer with soft tissue infection (39%), ulcer with osteomyelitis (32%), and puncture wounds (12%). Sixty percent of all patients had a second amputation, 21% had a third, and 7% had a fourth. Fifteen percent of the patients who had a second amputation had it contralaterally. Seventeen percent subsequently underwent a below-knee amputation and 11% had a Transmetatarsal amputation on the same extremity, 3% had a below-knee amputation, and 2% a transmetatarsal amputation contralaterally. The mean time from the first to the second amputation was approximately 10 months. The results of this study suggest that a large proportion of patients undergoing an amputation at the level of the great toe or first ray have subsequent amputations in the first year following the initial procedure. Additionally, it appears that the contralateral foot may be at significant risk for distal amputation following resection of the hallux or first day. 相似文献
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The hopscotch finite-difference technique is shown to be a fast and accurate way to simulate transient, saturated, ground-water flow in relatively typical but heterogeneous 2D and 3D domains. The odd-even hopscotch (OEH) and line hopscotch methods are reviewed, and their implementation for saturated ground-water flow is presented. The OEH scheme, which is a second-order accurate explicit process, is efficient, requiring only six floating point operations per mesh node and time step, and is unconditionally stable (for saturated ground-water flow). Numerical experiments on typical 2D meshes (2,500 nodes) with synthetic, randomly heterogeneous hydraulic conductivity, suggest that the OEH process is approximately 1.5 times faster than the alternating direction implicit method and 3–4 times faster than the Crank-Nicolson implicit method using preconditioned conjugate gradient iteration. Similar experiments on medium-sized 3D meshes (87,500 nodes) suggest that the OEH process is between 7 and 10 times faster than the Crank-Nicolson preconditioned conjugate gradient method. Although the numerical results presented illustrate only typical test problem performance, they nevertheless clearly indicate promise for using OEH to simulate transient ground-water flow in 2D and, especially, 3D heterogeneous domains requiring fine spatial meshes. 相似文献