Carotenoids and Their Geometry Isomers in Selected Tropical Fruits

This study determined the carotenoids content in cherry tomato, pink guava, and red grapefruit pulps and juices. Cherry tomato pulp exhibited the highest β-carotene content whereas pink guava pulp had the highest lycopene content. However, β-carotene and lycopene contents in the studied fruit juices were lower than their pulps in the same sample portion. Interestingly, six to twelve cis-isomers of carotenoids were identified in the fruit pulps and juices studied. A higher number of trace amounts of cis-carotenoids was found in fruit pulps as compared to juices. Therefore, consumption of whole fruit is recommended as the studied fruit juices have lower carotenoids content.     

LCI (Life cycle inventory) analysis of fuels and electricity generation in Singapore

This article offers a unique three-stage approach in LCI analysis for generating the environmental profile of electricity generation in Singapore. The first stage focuses on fuels delivered to Singapore, next on electricity generated from various types of power production plants. The third stage integrates the entire life cycle study. The final gate-to-gate results show that the total CO₂ emissions from the national grid are 455.6 kg CO₂ per MWh without any loss in transmission and 467.0 kg CO₂ per MWh with 2.5% losses. The results for the entire cradle-to-gate energy production are: 586.3 kg CO₂ per MWh without considering any losses and 601.0 kg CO₂ per MWh with 2.5% transmission loss. For the rest of the LCI, the cradle-to-gate results (per MWh) are kg 0.19 CO (carbon monoxide), 0.06 kg N₂O (nitrous oxide), 1.94–1.99 kg NO_x (nitrogen oxides), 2.94–3.01 kg SO_x (sulphur oxides), 0.064–0.066 kg VOC (volatile organic compounds) and 0.078–0.080 kg PM (particulate matters). From gate-to-gate, the results are 0.12 kg CO, 0.0016 kg N₂O, 1.42–1.46 kg NO_x, 2.56–2.62 kg SO_x, 0.033–0.034 kg VOC and 0.067–0.069 kg PM. Emissions of CO₂ from energy generation, climate change mitigation and policies for energy security are also discussed.     

Studies of sol-gel ceramic film incorporating methylene blue on glassy carbon: an electrocatalytic system for the simultaneous determination of ascorbic and uric acids

In this work, we investigated the immobilization of methylene blue in a methyltrimethoxysilane sol-gel ceramic film on a glassy carbon electrode. Up to a certain saturation level, under our conditions, it was found that the methylene blue was tightly held and did not leach out into aqueous solutions, even with continuous immersion for up to 1 month. The electrochemical behavior of the immobilized methylene blue was then studied. pH variation revealed that there were two distinct redox couples whose existences were pH-dependent. The methylene blue/sol-gel film was also examined as an electrocatalytic system for ascorbic and uric acid oxidations. It was revealed that this system was highly sensitive for ascorbic and uric acid sensing (practical determination limits of 5.00 nM and 1.00 nM for ascorbic acid and uric acid, respectively) and also allowed simultaneous determination of these biomolecules. The simultaneous determination of these two analytes in a human urine sample was demonstrated. The stability of the methylene blue/sol-gel film/glassy carbon electrode sensing system was good, with up to at least a month of continual operation.     

Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor-alpha

Mammalian tissues express three immunologically distinct peroxiredoxin (Prx) proteins (Prx I, II, and III), which are the products of distinct genes. With the use of recombinant proteins Prx I, II, and III, all have now been shown to possess peroxidase activity and to rely on Trx as a source of reducing equivalents for the reduction of H2O2. Prx I and II are cytosolic proteins, whereas Prx III is localized in mitochondria. Transient overexpression of Prx I or II in cultured cells showed that they were able to eliminate the intracellular H2O2 generated in response to growth factors. Moreover, the activation of nuclear factor kappaB (NFkappaB) induced by extracellularly added H2O2 or tumor necrosis factor-alpha was blocked by overproduction of Prx II. These results suggest that, together with glutathione peroxidase and catalase, Prx enzymes likely play an important role in eliminating peroxides generated during metabolism. In addition, Prx I and II might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentration of H2O2.     

Electrophysiological and neuropharmacological study of tectoreticular pathways in lampreys

Tectoreticular (TR) cells along the diencephalic-mesencephalic border are the origin of prominent crossed and uncrossed pathways that project to the middle (MRRN) and posterior (PRRN) rhombencephalic reticular nuclei in juvenile and adult lampreys [I.C. Zompa, R. Dubuc, Diencephalic and mesencephalic projections to rhombencephalic reticular nuclei in lampreys, Brain Res. (1998) in press.]. This study investigated the synaptic contacts between TR axons and the reticular cells. Intracellular recordings were carried out in reticular neurones (n=124) while microstimulating the TR regions. Tectoreticular inputs were recorded in all reticular cells studied (248 PSPs); although stronger responses were evoked in the MRRN neurones. The majority of responses were excitatory, but increasingly mixed and inhibitory when recorded in the middle and caudal part of the reticular nuclei. The excitation had the shortest onset latencies and sharpest slopes measured in both reticular nuclei, while the inhibition was longer and smoother. The characteristics of TR inputs to different reticular cell types is also presented. The transmission of evoked responses was isolated to the crossed and uncrossed TR pathways by studying the effects of 1% Xylocaine ejections and surgical lesions. The TR inputs were transmitted to reticular cells through monosynaptic and polysynaptic contacts. The synaptic transmission involved excitatory amino acids, acting through AMPA and NMDA receptors, while the inhibition was glycinergic. Comparisons with other sensory systems in lampreys are discussed.     

Cerebral microembolism in patients with retinal ischemia

BACKGROUND AND PURPOSE: We investigated the frequency of cerebral microembolism detected by transcranial Doppler ultrasonography in patients with clinical evidence of retinal ischemia, including transient monocular blindness, central and branch retinal artery infarction, and ischemic oculopathy, and assessed its correlation with carotid artery stenosis. METHODS: Records of 331 consecutive patients examined during a 47-month period at the Neurovascular Laboratory were reviewed. Of the original 453 intracranial arteries, 186 middle cerebral arteries (MCAs) satisfied qualifying criteria that excluded patients with cardiac embolic sources. Forty-five MCAs ipsilateral to the symptomatic eye constituted the study group. The control group consisted of 141 asymptomatic MCAs. Microembolus detection studies were performed on transcranial Doppler instruments equipped with special software, and the degree of carotid artery stenosis was measured by cerebral or MR angiography or by color duplex studies. RESULTS: Microembolism was detected in 40.0% of study MCAs and 9.2% of controls (P < 0.001). In the study group, microembolic signals were detected in 61.9% of MCAs tested within a week of symptom onset and 20.8% of those tested afterward (P < 0.001). Severe (> or = 70%) carotid stenosis or occlusion was more frequent in the study group (P < 0.001). Microembolic signals were detected in 25.3% and 11.2%, respectively, of MCAs distal to carotid arteries with 70% to 100% and 0% to 69% stenosis (P = 0.013). CONCLUSIONS: In patients without cardiac embolic sources, cerebral microembolism is frequently present on the side of retinal ischemia, particularly during the week after onset of symptoms. It is often associated with severe stenosis or occlusion of the ipsilateral carotid artery.     

Nasopharyngeal carcinoma with intracranial spread: CT and MR characteristics

PURPOSE: Nasopharyngeal carcinoma (NPC) frequently spreads intracranially. We compare CT and MRI in identifying intracranial spread and reexamine the route of infiltration. METHOD: One hundred fourteen consecutive patients with proven NPC were evaluated prospectively with T1-, T2-weighted, contrast-enhanced MRI and CT. RESULTS: MRI showed 35 (31%) patients with middle cranial fossa involvement. Twenty-nine (25%) patients had cavernous sinus infiltration, while six (5%) showed only dural thickening. The most common route of spread is through the foramen ovale (FO) (12/35 patients, 34%), followed by skull base destruction (6/35 patients, 17%), foramen lacerum (FL) (6/35 patients, 17%), sphenoid sinus (6/35 patients, 17%), and combined FO and FL (5/35 patients, 14%). Using MRI as a standard, CT demonstrated the following involvement: cavernous sinus in 26 of 29 (90%) patients, FO in 9 of 12 patients, skull base in 6 of 6 patients, FO and FL in 3 of 5 patients, FL in 6 of 6 patients, sphenoid sinus in 6 of 6 patients and dura in 0 of 18 patients. CONCLUSION: It is believed that NPC most commonly spreads intracranially via the FL or by direct erosion. Perineural spread through the FO is an important route, which explains why with CT evidence of cavernous sinus involvement there may be no skull base erosion. These findings are best seen on MRI.     

Dynamic response of deformable structures subjected to shock load and cavitation reload

The dynamic response of deformable structures subjected to shock load and cavitation reload has been simulated using a multiphase model, which consists of an interface capturing method and a one-fluid cavitation model. Fluid–structure interaction (FSI) is captured via a modified ghost fluid method (Liu et al. in J Comput Phys 190: 651–681, 2003), where the structure is assumed to be a hydro-elasto-plastic material if subjected to a strong shock load. Bulk cavitation near the structural surface is captured using an isentropic model (Liu et al. in J Comput Phys 201:80–108, 2004). The integrated multiphase model is validated by comparing numerical predictions with 1D analytical solutions, and with numerical solutions calculated using the cavitation acoustic finite element (CAFé) method (Sprague and Geers in Shocks vib 7:105–122, 2001). To assess the ability of the multiphase model for multi- dimensions, underwater explosions (UNDEX) near structures are computed. The importance of cavitation reloading and FSI is investigated. Comparisons of the predicted pressure time histories with different explosion center are shown, and the effect on the structure is discussed.     

Auto-charge pump for half-bridge inverters

The auto-charge pump is a self-starting and self-sustaining circuit used to generate a floating voltage supply for the high side circuitry of half-bridge inverters. The switching output of the half-bridge replaces the dedicated pump oscillator thus simplifying the circuit. A model is derived which is then verified experimentally