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VG Kolupaeva TV Pestova CU Hellen IN Shatsky 《Canadian Metallurgical Quarterly》1998,273(29):18599-18604
A complex of eukaryotic initiation factors (eIFs) 4A, 4E, and 4G (collectively termed eIF4F) plays a key role in recruiting mRNAs to ribosomes during translation initiation. The site of ribosomal entry onto most mRNAs is determined by interaction of the 5'-terminal cap with eIF4E; eIFs 4A and 4G may facilitate ribosomal entry by modifying mRNA structure near the cap and by interacting with ribosome-associated factors. eIF4G recruits uncapped encephalomyocarditis virus (EMCV) mRNA to ribosomes without the involvement of eIF4E by binding directly to the approximately 450-nucleotide long EMCV internal ribosome entry site (IRES). We have used chemical and enzymatic probing to map the eIF4G binding site to a structural element within the J-K domain of the EMCV IRES that consists of an oligo(A) loop at the junction of three helices. The oligo(A) loop itself is not sufficient to form stable complexes with eIF4G since alteration of its structural context abolished its interaction with eIF4G. Addition of wild type or trans-dominant mutant forms of eIF4A to binary IRES.eIF4G complexes did not further alter the pattern of chemical/enzymatic modification of the IRES. 相似文献
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锦屏一、二级电站对外交通专用公路羊房沟段部分边坡开挖后高度为35 m,地质条件极差,多为严重风化的岩石并夹杂大量泥沙,裂隙发育。为此,设计在此类路段使用1 000 kN级预应力锚索,配合3 m长锚杆对开挖边坡进行加固。锚索的注浆体为水泥净浆,锚索体为符合“预应力混凝土用钢绞线”GB/T 5224-2003和A STM A 416-94标准生产的1 860 M Pa高强度低松弛钢绞线。介绍了该段中K 46 319.84~K 46 520.16段工程施工中的锚索施工技术。 相似文献
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S Muzaffar IN Soomro N Kayani S Siddiqui YA Husein R Jooma 《Canadian Metallurgical Quarterly》1998,48(5):148-149
During my nursing career, I remember constantly being aware of patients who needed some emotional help--those who were frightened about a forthcoming operation, those who were shocked and despairing after being given their diagnosis and prognosis, others who were down in the dumps because they couldn't go home as soon as they had hoped. There were also the 'ward clowns' who tried to make everyone laugh with their good humour and little pranks, yet felt no less anxious, worried or depressed than anyone else. Patients seem to fit into categories: the nervous ones, the depressives, the jovial types, the moaners, those who demand attention and those who shun it. I feel sure that every nurse has noticed the different 'types' of people who fill hospital beds-ordinary people who seem to take on a new persona as soon as they get into their pyjamas and become a 'patient'. Somehow, their identity gets folded up and put away in their locker along with their outdoor clothes and other reminders of the outside world. 相似文献
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