Effect of structural analogs of platelet activating factor on the intracellular signal transduction in murine peritoneal neutrophils and macrophages of P388D1 line

Direct and modulate effects of platelet activating factor (PAF), its structural analogues and ATP on primary and second processes at peritoneal neutrophils and P388D1 cells activation has been studied. The effect of compounds was evaluated on changes in Ca2+ transport and generation of reactive oxygen species. It was shown, that the synthetic analogues of MS series interact with PAF receptor, mobilize Ca2+ from thapsigargin-dependent intracellular stores and inhibit Ca2+ response on PAF in both types of cells. Unlike PAF the analogues do not induce the formations of reactive oxygen species in neutrophils and inhibit the PMA-induced respiratory burst. The activation of pyrinoreceptor of P388D1 cells by exogenous ATP does not inhibit PAF induced Ca2+ rise in cytoplasm, though partly releases Ca2+ from the same store.     

A hydrodynamic model of a cochlea with a discrete basilar membrane]

The hydrodynamic model of a cochlea (dimensions 520 x 120 x 100 (mm)), with the basilar membrane (BM) consisting of 50 discrete resonance links in the frequency band from 1 Hz to 128 Hz was constructed. Two ways of giving harmonic and impulse's signals at the model were used; a) through the membrane of the vestibular window (directly through the filled model liquid along the BM) and b) through the thread fastened to the basal part of the BM (by the travelling wave on the BM). It was shown that in the first case the transmission's time to apical part of the BM equals 0.8 ms and in the second--several seconds. The conclusion is that the main hydrodynamic processes of sound's coding in a cochlea are the processes according to the Helmholtz's resonance theory, but not Békésy's travelling wave theory.     

Detection of alpha 1-antitrypsin in unfertilized ova of albino rats

500--700 rat oocytes were solubilized in 0.3--0.6 ml of 1% sodium dodecyl sulphate (SDS) --0.06 tris-HCl, pH 6.7. The excess of SDS was removed in the form of unsoluble Ba2 (SDS) and the lysate was placed on the surface of 1% agarose microgel, performed in a glass capillar with the inner diameter 600 and the length of gel 7--8 nm. The microvolume (1.3 mcl) of monospecific antiserum against alpha1-human antitrypsin was placed on the opposite surface of the agarose gel. After 48 hours, the amount of microimmunoprecipitate was determined by staining the gel with Amido Black 10 B, followed by quantitative scanning using a double wave microspectrophotometer. The mean value of alpha1-antitrypsin was about 2.3+/-1.4 pcg per ovum. It is supposed that this antienzyme may terminate the "zone" and "cortical" reactions during the fertilization in mammals.     

The nature of the changes in the immunity of the women who worked in the cleanup of the sequelae of the accident at the Chernobyl Atomic Electric Power Station and were evacuated from the radioactively contaminated area

During examination of 37 women taking part in elimination of consequences of Chernobyl's disaster was revealed, that complex of disaster factors action resulted the formation of disbalance in the system of immunocompetence cells and caused the oppression of functional activity of neutrophils. In 110 women living on the radioactive contaminated zone (5-40 Ci/km) the phenomena of the secondary immunodeficiency in the form of defeat as cellular as humoral factors of immunity and nonspecific resistance system was diagnosed.     

The structure of a population of smooth myocytes (aspects of the intraorganic organization of smooth muscle tissue)

In this qualitative study, we have analysed the communication established between mother-child and the nursing auxiliary personal in situations of injection medication in children. Data were classified according to the FORREST Theoretical Model. The non participative observation method was used with field note in twelve situations. The prevalence of blocking categories was confirmed as Disapproval, Depreciation and Tranquilizing the mother with stereotyped comments. The facilitating categories show subcategories such as recognizing feelings and presence of child clarifying and orientating mother or the responsible person. Authors concluded that there was prevalence of blocking categories when verbal communication resources were not used to interact with mother and child, without establishing a relationship of friendship and confidence and making the relation ineffective.     

Evaluation of individual radiation resistance of rats based on reactions to non-radiation testing

Presented are the data on radiation sensitivity of various groups of animals preliminary differentiated by their tolerance of acute hypoxia. The processes of blood forming system impairment and reparation are detailed. As was shown, highly resistant to hypoxia rats are distinguished by the best radiation resistance. Survivability of these rats was significantly higher as compared with other groups of animals. Recovery of blood formation by both the red and white chits following exposure to [symbol: see text] of the mean lethal dose proceeded more rapidly in the radiation resistant rats.