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41.
42.
Martin Meyer 《Scientometrics》2007,70(3):779-810
This contribution formulates a number of propositions about the emergence of novel nanoscience and nanotechnology (N&N). Seeking
to complement recent work that aims to define a research agenda and draws on general insights from the innovation literature,
this paper aims to synthesize knowledge from innovation-related studies of the N&N field. More specifically, it is suggested
that N&N is often misconstrued as either a field of technology or an area of (broadly) converging technologies while evidence
to date suggests rather that N&N be considered a set of inter-related and overlapping about not necessarily merging technologies.
The role of instrumentation in connecting the various N&N fields is underlined. Finally, the question is raised whether change
in N&N tends to be incremental rather than discontinuous, being the result of technological path-dependencies and lock-ins
in industry-typical search regimes that are only slowly giving way to more boundary-crossing activities. 相似文献
43.
R. Meyer Hilde Hardtdegen R. Carius D. Grützmacher M. Stollenwerk P. Balk A. Kux B. Meyer 《Journal of Electronic Materials》1992,21(3):293-298
This paper presents a study of the structural and optical properties of strained GaInAs/ InP multiple quantum well (MQW) structures
fabricated by LP-MOVPE. The composition of the Ga
x
In1−x
As films ranged fromx = 0.17 tox = 1.0 and was determined by sputtered neutral mass spectrometry (SNMS) on thick layers. The structures of the MQW samples
with well widths from 1.5 to 5 nm were investigated by high resolution x-ray diffraction (HR-XRD). Simulations of the diffraction
patterns showed that transition layers of approximately 2 monolayer (ML) thickness with high lattice mismatch exist at the
interfaces. Photoluminescence (PL) measurements indicate well widths of a multiple of a monolayer with local variations of
one monolayer. The PL peak energies vary smoothly with the Ga concentration. These results were confirmed by optical absorption
measurements. 相似文献
44.
Syncytium-inducing (SI) variants of human immunodeficiency virus type 1 (HIV-1) are evolutionary variants that are associated with rapid CD4+ cell loss and rapid disease progression. The heteroduplex tracking assay (HTA) was used to detect evolutionary V3 variants by amplifying the V3 sequences from viral RNA derived from 50 samples of patient plasma. For this V3-specific HTA (V3-HTA), heteroduplexes were formed between the patient V3 sequences and a probe with the subtype B consensus V3 sequence. Evolution was then measured by divergence from the consensus. The presence of evolutionary variants was correlated with SI detection data on the same samples from the MT-2 cell culture assay. Evolutionary variants were correlated with the SI phenotype in 88% of the samples, and 96% of the SI samples contained evolutionary variants. In most cases the evolutionary V3 variants represented discrete clonal outgrowths of virus. Sequence analysis of the six discordant samples that did not show this correlation indicated that three non-syncytium-inducing (NSI) samples had V3 sequences that had evolved away from the consensus sequence but not toward an SI genotype. A fourth sample showed little evolution away from the consensus but was SI, which indicates that not all SI variants require basic substitutions in V3. The other two samples had SI-like genotypes and NSI phenotypes, suggesting that V3-HTA was able to detect SI emergence in these samples in the absence of their detection in vitro. V3-HTA was also used to confirm SI variant selection in MT-2 cells and to examine the possibility of variant selection during virus culture in peripheral blood cells. 相似文献
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Brain injury induces reactive gliosis, characterized by increased expression of glial fibrillary acidic protein (GFAP), astrocyte hypertrophy, and hyperplasia of astrocytes and microglia. One hypothesis tested in this study was whether ganglioside GD3+ glial precursor cells would contribute to macroglial proliferation following injury. Adult rats received a cortical stab wound. Proliferating cells were identified by immunostaining for proliferating cell nuclear antigen (PCNA) and by [3H]-thymidine autoradiography, and cell phenotypes by immunocytochemical staining for GD3, GFAP, ED1 (for reactive microglia) and for Bandeiraea Simplicifolia isolectin-B4 binding (all microglia). Animals were labeled with thymidine at 1,2,3, and 4 days postlesion (dpl) and sacrificed at various times thereafter. Proliferating cells of each phenotype were quantified. A dramatic upregulation of GD3 on ramified microglia was seen in the ipsilateral hemisphere by 2 dpl. Proliferating cells consisted of microglia and fewer astrocytes. Microglia proliferated maximally at 2-3 dpl and one third to one half were GD3+. Astrocytes proliferated maximally at 3-4 dpl, and some were also GD3+. Both ramified and ameboid forms of microglia proliferated and by 4 dpl all GD3+ microglia were ED1+ and vice versa. In the contralateral cortex microglia expressed neither GD3 nor ED1. Thus they acquired these antigens when activated. Neither microglia nor astrocytes that were thymidine-labeled at 2, 3, or 4 dpl changed in number in subsequent days. Most thymidine+ astrocytes were large GFAP+ reactive cells that clearly arose from pre-existing astrocytes, not from GD3+ glial precursors. In this model of injury microglia proliferate earlier and to a much greater extent than astrocytes, they can divide when in ramified form, and GD3 is up-regulated in most reactive microglia and in a subset of reactive astrocytes. We also conclude that microglial proliferation precedes proliferation of invading blood-borne macrophages. 相似文献
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