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991.
Mastitis occurrence within a lactation and times of mastitis onset were studied for about 1000 cows. The number of mastitis cases within a lactation was modeled through overdispersed Poisson regression with individual and herd covariants. The results emphasized the role of the herd variable. Increased production potential increased the number of cases per lactation at a rate of 1.4/10 kg. Calving month also played an important role. The incidence of mastitis was greater when calving took place in early autumn or winter, which led to an expanded housing period. The interval from calving to the first case of mastitis and the intervals between successive cases were modeled for cases occurring during lactation through random selections from fitted gamma distributions, these distributions being truncated to consider the lactation length. The results of both steps can be used to simulate mastitis occurrence in different conditions. 相似文献
992.
Saccharomyces cerevisiae (Sc) mRNAs have been described as falling into two major classes with respect to mRNA half-life [Santiago et al., Nucleic Acids Res. 14 (1986) 8347-8360]. We have used DNA sequence analysis to address the functional roles of eleven of the thirteen cDNAs upon which Santiago et al. based their conclusions. Eight had been described as copies of short half-life and five as copies of long-half-life mRNAs. We show here that five members of the short-half-life class encode known Sc cytosolic ribosomal proteins (rp). One further short-half-life cDNA appears to encode a new Sc rp related to higher eukaryotic rp S12. Among the long-half-life cDNAs, one encodes the glucose-inducible glycolytic enzyme enolase, while another is related to the mouse housekeeping gene MER5. 相似文献
993.
994.
The distribution of antigen-specific memory T cells in different lymph nodes of sheep was determined using an antigen-specific in vitro proliferation assay. Lymph nodes were collected from sheep immunized simultaneously with avidin or ovalbumin in a peripheral tissue site (hind leg muscle) and keyhole limpet haemocyanin (KLH) in an intestinal tissue site (gut wall or colonic mucosa). The results showed a consistently high proliferative response in typical peripheral lymph nodes (popliteal and prescapular) and a low or negative response in gastrointestinal lymph nodes (abomasal and jejunal) while the response in other nodes was variable. The low proliferative response in the gastrointestinal lymph nodes was not due to the presence of suppressor CD8- lymphocytes and the proliferative response could not be raised to peripheral lymph nodes levels with the addition to cultures of IL-2 or mitomycin-C treated peripheral lymph node cells. The high proliferative response in the peripheral lymph nodes was not suppressed by the addition of mitomycin-C-treated gastric lymph node cells but was dramatically reduced by the addition of mAb against the IL-2-receptor or by depletion of CD4- T cells. The results suggest that antigen-specific proliferative memory T cells, which may be Th1-like memory cells, preferentially migrate to peripheral lymph nodes independent of their site of induction. 相似文献
995.
MA Knepper JB Wade J Terris CA Ecelbarger D Marples B Mandon CL Chou BK Kishore S Nielsen 《Canadian Metallurgical Quarterly》1996,49(6):1712-1717
Aquaporins (AQPs) are a newly recognized family of transmembrane proteins that function as molecular water channels. At least four aquaporins are expressed in the kidney where they mediate rapid water transport across water-permeable epithelia and play critical roles in urinary concentrating and diluting processes. AQP1 is constitutively expressed at extremely high levels in the proximal tubule and descending limb of Henle's loop. AQP2, -3 and -4 are expressed predominantly in the collecting duct system. AQP2 is the predominant water channel in the apical plasma membrane and AQP3 and -4 are found in the basolateral plasma membrane. Short-term regulation of collecting duct water permeability by vasopressin is largely a consequence of regulated trafficking of AQP2-containing vesicles to and from the apical plasma membrane. 相似文献
996.
KG Miller A Alfonso M Nguyen JA Crowell CD Johnson JB Rand 《Canadian Metallurgical Quarterly》1996,93(22):12593-12598
We have isolated 165 Caenorhabditis elegans mutants, representing 21 genes, that are resistant to inhibitors of cholinesterase (Ric mutants). Since mutations in 20 of the genes appear not to affect acetylcholine reception, we suggest that reduced acetylcholine release contributes to the Ric phenotype of most Ric mutants. Mutations in 15 of the genes lead to defects in a gamma-aminobutyric acid-dependent behavior; these genes are likely to encode proteins with general, rather than cholinergic-specific, roles in synaptic transmission. Ten of the genes have been cloned. Seven encode homologs of proteins that function in the synaptic vesicle cycle: two encode cholinergic-specific proteins, while five encode general presynaptic proteins. Two other Ric genes encode homologs of G-protein signaling molecules. Our assessment of synaptic function in Ric mutants, combined with the homologies of some Ric mutants to presynaptic proteins, suggests that the analysis of Ric genes will continue to yield insights into the regulation and functioning of synapses. 相似文献
997.
JD Pierce CJ Wysocki EV Aronov JB Webb RM Boden 《Canadian Metallurgical Quarterly》1996,21(2):223-237
Cross-adaptation, the decrease in sensitivity to one odorant following exposure to a different odorant, is affected by odorant similarity, both perceptual and structural, but the precise relationship is obscure. The present series of studies was designed to explore various aspects of perceptual and structural similarity as they relate to cross-adaptation. In Experiment 1, cross-adaptation was assessed between androstenone and five odorants that share a common urinous note with androstenone, but retain unique perceptual characteristics; only the compound judged most perceptually similar to androstenone cross-adapted it. In Experiment 2, odorants both perceptually and structurally similar (androstenone and androstanone) displayed significant, mutual cross-adaptation. Furthermore, magnitude estimates for androstanone were significantly reduced following exposure to 3-methylidene-5 alpha-androstane (3M5A), a structurally similar, perceptually odorless compound. This finding appears to be the first demonstration that an odorless compound can affect, via cross-adaptation, the perception of an odorous compound. Finally, in Experiment 3, significant, asymmetric cross-adaptation was observed between compounds that are perceptually and structurally dissimilar (4-cyclohexylcyclohexanone [4-CHCH] and androstenone). These findings indicate that the role of similarity in cross-adaptation is difficult to quantify and emphasize the numerous odorant characteristics that can affect cross-adaptation. 相似文献
998.
Fluorescence detection of fluorogen-labeled neurotransmitters is demonstrated using 100 fs pulses from a titanium-sapphire mode-locked laser to achieve molecular excitation by simultaneous absorption of two and three photons of near-IR radiation. Two-photon excitation spectra are determined for the naphthalene-2,3 dicarboxaldehyde derivative of glycine and the fluorescamine derivative of leucine enkephalin, with the peak excitation cross section (o2) approximately equal to 1 x 10(-50) cm4 s/photon for both species. Three-photon-excitation fluorescence is demonstrated for o-phthaldialdehyde-labeled glutamate using excitation wavelengths between 965 and 1012 nm. The three-photon excitation cross section (o3) remains nearly constant in this wavelength range, with an absolute value of approximately 10(-84)-10(-85) cm6 s2/photon 2. Rapid cycling of analytes through the fluorescent excited state and detection that is free from background caused by Rayleigh and Raman scatter combine to make multiphoton-excited fluorescence a highly sensitive approach for detecting trace amounts of neurotransmitters. Measurements of two-photon-excited fluorescence of fluorescamine-labeled bradykinin and analysis of multiphoton-excited background reveal the potential of this method to detect fewer than 1000 neurotransmitter molecules. 相似文献
999.
1000.
Kosslyn Stephen M.; Chabris Christopher F.; Marsolek Chad J.; Jacobs Robert A.; Koenig Olivier 《Canadian Metallurgical Quarterly》1995,21(2):423
Computational models in psychology play an increasingly important role in characterizing theoretical distinctions, understanding empirical results, and formulating new predictions. However, the proper use of models is subject to debate and interpretation, as Cook, Früh, and Landis (see record 1995-31404-001) have demonstrated in a critique of neural network simulations reported by Kosslyn, Chabris, Marsolek, and Koenig (see record 1992-37420-001). These simulation results supported a distinction between two types of spatial relations encoding. Cook et al argue that Kosslyn et al's models did not process "spatial" representations and that input–output correlations rather than properties of spatial relations encoding processes explain the performance of the models. This article provides conceptual and analytic rebuttals of those criticisms. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献