Sequence analysis of the CCG polymorphic region adjacent to the CAG triplet repeat of the HD gene in normal and HD chromosomes

The CAG expansion responsible for Huntington's disease (HD) is followed by an adjacent polymorphic CCG repeat region which may interfere with a PCR based diagnosis. We have sequenced this region in 52 unrelated HD patients, from both normal and HD chromosomes. Fifty percent of the normal alleles were (CCG)7(CCT)2, 48% (CCG)10(CCT)2, and 2% (CCG)7(CCT)3. In contrast (CCG)7(CCT)2 was found in 85% of the HD alleles which represents significant linkage disequilibrium with the HD mutation.     

Direct evidence for the role of haem doming as the primary event in the cooperative transition of haemoglobin

The study of cooperative ligand binding among the four subunits of haemoglobin has played a central role in the understanding of allosteric transitions in a large number of enzymes. Haem iron out-of-plane motion has been suggested to be the trigger for the cooperative transition of haemoglobin. To function as a trigger in a dynamic sense, haem-iron doming must be the first conformational change to occur following ligand dissociation. Here we present the first direct demonstration that haem-iron doming occurs on the same time scale as the breaking of the iron-ligand bond, thus establishing haem-iron doming as the primary event which lead to the R-->T transition in haemoglobin.     

Use of a multileaf collimator for the production of intensity-modulated beams

The continuous release of nitric oxide (NO) from the constitutive, endothelial isoform of nitric oxide synthase (e-NOS) serves mainly to keep the vasculature in a continuous state of active vasodilation. Although it has been suggested that NO production from e-NOS might also be affected by hemorrhagic shock (HS), this relationship is still controversial. Therefore, the roles of NO in the pathophysiology in hemorrhagic shock were reviewed. According to the previous reports, NO might play an important role in the pathophysioliogy of HS. In the early phase of HS, it may be possible that NO delivered from e-NOS serves a cytoprotective function in preventing shock-induced organ injury. This opinion suggests that endothelial NO production has a significant modulatory effect on vascular tone during hemorrhage, and that inhibition of NO production permits greater vasconstrictor influences leading to organ injury. NO production in the late phases of HS has an adverse effect on survival rate in the HS model. Moreover, the findings from an animal study of prolonged periods of HS suggest that excessive NO formation, including those produced from i-NOS, induces vascular hypoactivity and they have suggested that NOS inhibitors may improve the therapeutic outcome for patients suffering from HS. Therefore, it may be suggested that NO might play a biphasic role, cytoprotective during the early phase and cytotoxic late in HS.     

Effects of growth hormone, insulin-like growth factor-I, and cortisol on periparturient antibody response profiles of dairy cattle

The objectives of this study were to determine hormone and antibody response profiles from the prepartum period to peak lactation, and evaluate potential immunomodulatory effects of the classic endocrine hormones, growth hormone (GH), insulin-like growth factor-I (IGF-I) and cortisol. Specifically, 33 Holstein cows were immunized with ovalbumin (OVA) and Escherichia coli J5 at weeks -8 and -3 prior to parturition. At parturition (week 0), cows received an additional immunization of OVA. Blood was collected at weeks -8, -3, 0, 3 and 6 relative to parturition and various samples were used to determine plasma hormone concentration, serum immunoglobulin (Ig), and specific antibody response to OVA and E. coli. Colostrum and milk samples were also collected post-parturition to monitor local immunoglobulin and antibody responses. Results indicated that not all periparturient cows exhibited depressed immune response, and that antibody response to OVA could be used to partition cows into 3 groups recognizing animals with sustained measurable antibody response before and after parturition (Group 1), animals which responded poorly to immunization at parturition (Group 2), and animals which did not respond to immunizations at week -3 or parturition (Group 3). Cows with the highest antibody response to OVA (Group 1) also tended (P < or = 0.10) to have the highest response to E. coli J5 at parturition and had the lowest incidence of disease, particularly mastitis. Antibody response to OVA measured in milk tended to be higher in Group 1 cows, particularly at week 0 (P < or = 0.06) compared to cows of Group 3. IGF-I was higher (P < or = 0.05) in cows of Group 1 than Group 3 at peak lactation (week 6).     

改进光电晶体管线性运行的反馈电路

当设计者用光电晶体管将一个调制后的光信号转换成电信号时,如果有高亮度的背光使光电晶体管饱和,就会遇到麻烦.当光电晶体管基极端悬浮时,其集电极/射极电压只取决于信号与背光重叠而产生的光电流.光电晶体管增益及其作用区范围由R1阻值(见图1)确定.R1阻值较高时,电路的增益增加,但光电晶体管会很快饱和.图1中没有背光照射时,晶体管工作在其线性区的偏置点φ2,Q1的集电极电压围绕VCE作线性变化.其输出VOUT准确地重复使调制光信号产生幅度波动.当施加外部稳定的背光照明时,电路的工作点移至偏置点φ3,输出电压被压缩并出现失真.     

探讨RPU整理剂在针织面料中的应用

介绍了针织面料弹性整理的反应机理,探讨RPU弹性整理工艺中RPU整理剂的用量、定形温度、整理工艺对整理效果的影响,从而确定最佳应用工艺条件.试验结果表明:经RPU弹性整理后的面料赋予卓越的弹性回复及优异的吸水性等效果,且手感有很大提高,服用性能优于其它整理剂.     

Efficiency of modified urea fertilizers for tropical irrigated rice

An objective of the International Network on Soil Fertility and Fertilizer Evaluation for Rice (INSFFER) network is to field evaluate deep-point placement (urea supergranules) and slow-release (sulfur coated urea) N fertilizers in irrigated rice. These N sources were compared for performance with split application of prilled urea at 19 sites in Asia in wet season 1981.SCU or USG differed significantly in response curves from prilled urea at 12 of the 17 sites where N response was observed. Over these 17 sites, 22–25% less N as SCU or 29–31% less N as USG provided the same yield increment as the comparatively higher level of N as prilled urea.High profit N rates were derived for 5 sites. The optimal N levels for SCU or USG were less than for prilled urea. However, in one case for both test materials prilled urea was more profitable than SCU or USG. The marginal rates of return of using SCU or USG as opposed to OPU were calculated for the 11 sites where the response functions of the test materials differed significantly from prilled urea. In other than 2 sites for SCU the MRR exceeded 2.0 for 29 and 58 kg N ha^–1, indicating the general profitability of these materials when compared to prilled urea.