Cryoimmunoglobulin IgGk with microtubular ultrastructure associated with pyoderma gangrenosum

Based on the hitherto published population data of the human red cell PGM1 and acid phosphatase polymorphisms, the geographical distributions of their gene frequencies were analyzed. As far as the acid phosphatase alleles are concerned, a marked geographical gradient was found as the Pa and Pb alleles showed significant correlations with the mean annual temperatures of the various human biotopes (Pa:r = -0.706; Pb:r = +0.812). Against that, the world distribution of the PGM1 alleles did not show a comparable correlation (PGM1 1:R = +0.063; PGM2 1:R = -0.063). The possible reasons for the distribution pattern of the acid phosphatase alleles are discussed.     

Posterior roentgenogram of the foot

A present there is no reliable way to define the position of the heel in relationship to the tibia during weight bearing. Many surgical and nonsurgical procedures are done to correct a varus or valgus heel without a standard method to define the problem or measure the results. This is a report on a posterior roentgenographic technique which defines the position of the heel with respect to the talus, tibia, and forefoot. This technique allows for measurement of the position of the heel and can be performed in any X-ray facility.     

Surgical salvage after radiation for laryngeal cancer

From January, 1962, through December, 1973, 1,084 patients with cancer of the intrinsic larynx were treated at the Mayo Clinic. Of the 1,084 patients, 136 were retreated after radiation therapy failed to cure their cancer (105 glottic, 30 supraglottic, and one subglottic cancers). Recurrence of glottic cancer was generally recognized later than recurrences in the supraglottic area; likewise, glottic cancers were more advanced at recurrence than supraglottic growths. When feasible, conservation surgery was carried out on both glottic and supraglottic growths. Unfortunately, many growths were too advanced for conservation surgery by the time recurrence was recognized. The concept of "radiate-and-watch" for early glottic and supraglottic cancers is designed to save larynges. The concept is not supported by this study.     

Studies on the metabolism of Orange Rn in the pig

Orange RN (monosodium salt of 1-phenylazo-2-naphthol-6-sulphonic acid) was administered to female pigs either by intraveneous injection or by stomach tube. After intravenous injection of Orange RN, 7.8 mg/kg, the following metabolites were indentified in 24-hour urines: Orange RN (31%), 1-(4-hydroxyphenlazo)-2-naphthol-6-sulphonic acid (3%), p-aminophenol and 0-aminophenol (34% and 4% of the theoretical yield, respectively). 1-Amino-2-naphthol-6-sulphonic acid was present, but not determined. When the urine collection was extended to 72 hours the excretion of p-aminophenol accounted for the remainder of the phenylazo moiety. The excretion pattern for p-aminophenol suggest that Orange RN is partly excreted in the bile and thereafter undergoes azo reduction in the gut. After administration of Orange RN, 78 mg/kg, by stomach tube the following metabolites were identified in the urine: Total coloured metabolites (Orange RN and 1-(4-hydroxyphenylazo)-2-naphtholphenol-6-sulphinic acid) (0.4% of the theoretical yield), p-aminophenol and o-aminophenol (52% and 6% of the theoretical yield, respectively) and aniline (0.3% of the theoretical yield). 1-Amino-2-naphthol-6-sulphonic acid was present, but not determined.     

Effects of continuous light and darkness on the eyes of the troglobitic salamander Typhlotriton spelaeus

Larval Typhlotriton spelaeus collected from five caves in Pulaski Co., Missouri, were kept as larvae or induced to transform in darkness or continuous fluorescent illumination. Larvae maintained in darkness for 215 and 279 days had smaller eyes, smaller rod inner and outer segments, and fewer metaphase figures in the germinative zone of the neural retina than comparable larvae maintained in light (258 lux). Except for visual cell size, differences were small and for each characteristic exceptions were observed. One larva kept in light showed early retinal degeneration comparable to that in transformed adults to T. spelaeus. All larvae exhibited optomotor behavior both before and after the experiment. Among animals induced to transform by L-thyroxin and maintained in darkness 111 to 366 days, visual cell and pigment epithelium degeneration was more extensive and more frequent than in animals kept for the same length of time in light (237-298 lux). In darkness the frequency of animals with retinal degeneration increased between 111 and 366 days. In light some animals exhibited pigment epithelium reduction with normal visual cells, and others had free, pigmented cells in the subretinal space. These effects were not comparable to degeneration in darkness. Eyelids covered the eyes of only a few animals in both light and dark treatments. The extent of eyelid encroachment over the eye greater in darkness than in light. Most animals exhibited optomotor responses after experiments, but responses of animals kept in darkness were impaired in comparison to those of animals kept in light.