Molecular dynamics simulations of the docking of substituted N5- deazapterins to dihydrofolate reductase

Orientations of the deazapterin ring and the conformational preferences of groups appended to the deazapterin ring in a set of 8-substituted deazapterin cations docked into the dihydrofolate reductase (DHFR) binding site have been investigated using a methodology based on the simulated annealing technique within molecular dynamics (MD) simulations. Of five possible binding pockets for the 8-substituents, identified from a preliminary manual docking study, one has been definitively eliminated after an analysis of MD trajectories, while another remains uncertain. Using a new method based on standard thermodynamic cycles and a linear approximation of polar and non-polar free energy contributions from MD averages, binding affinities of the different ligands in each binding site have been correlated with experimental dissociation constants. The study has provided insights into structure-activity relationships for use in the design of modified inhibitors of DHFR.      

Fighting Computer Crime with Software Risk Analysis

Executive management must have a sound basis for deciding how to control and protect business systems. The potential cost/risk of a system exposure must be quantified before an intelligent cost/benefit decision can be made. Risk analysis attempts to address this need by analyzing exposures in a systematic way. The authors describe one organization's application of risk analysis techniques to software systems.     

Alternative resist adhesion and electroplating layers for LIGA process

 The fabrication of high aspect ratio microstructures using the X-ray lithography and electroplating step of the LIGA process requires, that the metal layer on the substrate (plating base) enables a flawless resist adhesion and provides a high conductivity for the following electroplating process. In addition, a flawless metal adhesion after electroplating has to be ensured. Typically metal layers of titanium, but also of copper and gold are used. In case of higher structures, fluorescence radiation generated in the metal layer during exposure reduces the bond strength between the resist and the adhesion layer [7]. Since the fluorescence probability decreases with a decreasing atomic number, the suitability of conductive carbon as the plating base was investigated and compared to results achieved with titanium. The resist adhesion was increased tremendously on the carbon layer but the metal adhesion is insufficient. Therefore, platinum islands were deposited on the conductive carbon to facilitate good metal adhesion. The resulting resist adhesion was still superior compared to titanium. In addition, metal adhesion was achieved on these layers.     

LIGA micro-openings for coherence characterization of X-rays

Infinitely thin opaque screens serving for diaphragms as defined in the visible-light optics are not feasible for operation with X-ray beams due to their high penetrability. Micro-openings of predicted sidewall shape in a gold layer up to 140 μm thick which would provide low transparency, are proposed. The proposed micro-openings were made using the LIGA technique and tested successfully at photon energies of up to 25 keV. The micro-openings can be used as targets for coherence X-ray pattern or, if long interference tails are avoided by means of the advanced sidewall shape, as X-ray beam collimators.     

Situative Managementunterstützungssysteme

Digital natives increasingly populate organizations?? management. These new-generation managers more naturally accept management support systems (MSS), but also have higher expectations about how they should accommodate their individual user preferences. As a result, managers question MSS that have been developed without configuration mechanisms to accommodate their working style, relevant MSS use cases, and different MSS access modes. The objective of this article is to reveal managers?? different MSS use situations and propose levers for tailoring (conceptual) MSS design to them. Use situations generalize classes of similar user-group preferences. We first apply findings from a literature review to cluster managers?? user-group preferences into 36 MSS use situations. Second, we propose that the selection of end-user devices can serve as a main lever for MSS configuration. Third, we complete the configuration with a MSS user-interface design. Finally, we demonstrate utility of our configuration model by presenting and evaluating a prototype.     

Enabling resilient P2P video streaming: survey and analysis

Peer-to-Peer (P2P) techniques for multimedia streaming have been shown to be a good enhancement to the traditional client/server methods when trying to reduce costs and increase robustness. Due to the fact that P2P systems are highly dynamic, the main challenge that has to be addressed remains supporting the general resilience of the system. Various challenges arise when building a resilient P2P streaming system, such as network failures and system dynamics. In this paper, we first classify the different challenges that face P2P streaming and then present and analyze the possible countermeasures. We classify resilience mechanisms as either core mechanisms, which are part of the system, or as cross-layer mechanisms that use information from different communication layers, which might inflict additional costs. We analyze and present resilience mechanisms from an engineering point of view, such that a system engineer can use our analysis as a guide to build a resilient P2P streaming system with different mechanisms and for various application scenarios.     

Stiction issues and actuation of RF LIGA-MEMS variable capacitors

High aspect ratio variable capacitors have been fabricated using deep X-ray lithography and electroplating. Stiction phenomena applicable to high aspect ratio devices are presented, including the conditions for stiction to occur and the critical dimensions of structures. Actuation tests at 3 GHz are also presented and show a maximum capacitance of 0.86 pF with no actuation voltage and a minimum capacitance of 0.70 pF with an actuation voltage of 20 V just before pull-in, which gives a tuning range of 1.23:1. Corresponding Q-factor values are 49.3 and 70.8 respectively. After pull-in, the measured capacitance is 0.61 pF, corresponding to a tuning range of 1.41:1, with a maximum Q-factor of 102.9.     

Development of miniaturized piezoelectric actuators for opticalapplications realized using LIGA technology

To combine the advantages of the LIGA technology and piezoelectric materials, first prototypes of movable elastic LIGA structures have been realized on piezoelectric substrates. The structures consist of a flexible beam fixed to the piezoelectric substrate at its two ends in order to amplify the d₃₁-contraction of the piezoelectric substrate. By using this flexible LIGA beam as a magnification mechanism, the small displacements of the piezoelectric substrate are amplified by more than a factor of 20, offering various possibilities of applications, especially in micro-optics. In this paper, first the fabrication process and the performance of these new prototypes of LIGA piezoelectric actuators are described in detail, demonstrating the feasibility of the new concept combining the LIGA technique and piezoelectric substrates. Subsequently, LIGA-piezoelectric actuators developed for micro-optical applications are presented. These new optical actuators are also based on an elastic beam but slightly modified in order to modulate light propagating in optical fibers. They find promising applications as micro-optical attenuators or choppers whose performance is demonstrated