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51.
F Espa?a M Martínez J Sánchez-Cuenca CD Vera A Estellés JF Jiménez-Cruz 《Canadian Metallurgical Quarterly》1996,30(4):512-518
OBJECTIVE: To improve the specificity and sensitivity of the prostate-specific-antigen (PSA) assay for the distinction between prostate cancer and benign prostate hyperplasia (BPH). METHODS: Two sensitive immunoassays, one that measures free PSA and PSA complexed to alpha 1-antichymotrypsin (alpha 1-ACT) with the same efficiency (PSAag assay) and another that specifically measures the complex between PSA and alpha 1-ACT, have been designed to measure the PSA forms in the plasma of 84 patients with prostate disease and in the seminal plasma from 60 healthy individuals. RESULTS: The proportion of plasma PSA in complex with alpha 1-ACT was significantly higher in the 34 patients with prostate cancer (89 +/- 12%, mean +/- SD; median, 91%) than in the 50 patients with BPH (71 +/- 12%; 73%) and did not correlate with the total amount of PSA. Normal seminal plasma (n = 60) had 2.1 +/- 0.6 mg/ml PSA, 175 +/- 62 microns/ml alpha 1-ACT and 9.6 +/- 3.4 micrograms/ml PSA: alpha 1-ACT complex. CONCLUSION: These results confirm that PSA: alpha 1-ACT may be a good marker for a differential diagnosis of carcinoma of the prostate and BPH. 相似文献
52.
JF Allemand D Bensimon L Jullien A Bensimon V Croquette 《Canadian Metallurgical Quarterly》1997,73(4):2064-2070
Recent developments in the rapid sequencing, mapping, and analysis of DNA rely on the specific binding of DNA to specially treated surfaces. We show here that specific binding of DNA via its unmodified extremities can be achieved on a great variety of surfaces by a judicious choice of the pH. On hydrophobic surfaces the best binding efficiency is reached at a pH of approximately 5.5. At that pH a approximately 40-kbp DNA is 10 times more likely to bind by an extremity than by a midsegment. A model is proposed to account for the differential adsorption of the molecule extremities and midsection as a function of pH. The pH-dependent specific binding can be used to align anchored DNA molecules by a receding meniscus, a process called molecular combing. The resulting properties of the combed molecules will be discussed. 相似文献
53.
54.
1. A major issue facing nursing, and society as a whole, is the longstanding and continuing nursing shortage in long-term care. 2. The demand for RNs in nursing homes will continue to intensify into the next century as the population of older adults with complex care needs continues to grow. 3. The findings of this study suggest that RN recruitment needs in long-term care include having enough qualified and dedicated staff, supportive and competent administration; competitive salaries and benefits; functional, attractive facilities; improved professional and public image; a caring, supportive environment; realistic regulations; decreased paperwork; progressive nurse practice models; and opportunities for educational advancement and career growth. 相似文献
55.
56.
J Moawad JF McKinsey CW Wyble HS Bassiouny LB Schwartz BL Gewertz 《Canadian Metallurgical Quarterly》1997,132(6):613-8; discussion 618-9
57.
O Gisserot JP Jaureguiberry P Carli JF Paris D Jaubert A Chagnon 《Canadian Metallurgical Quarterly》1996,63(11):870-871
58.
SG Rijpkema RG Herbes N Verbeek-De Kruif JF Schellekens 《Canadian Metallurgical Quarterly》1996,117(3):563-566
Epiglottic disruption is an uncommon injury usually associated with significant supraglottic trauma. This injury may be overlooked because of the difficulty in examining the larynx or other associated severe injuries. We present two cases of clinically unsuspected epiglottic disruption that were first seen on MR images of the neck. 相似文献
59.
Treatment of hysteria requires establishing a management strategy. The first steps consist of symptomatic treatment (catharsis, counter-suggestion, hypnosis, narco-analysis, relaxation, focused psychoanalytical psychotherapy, drug therapy). Thereafter, an approach aiming to modify the psychic structure of the patient can be considered (supportive psychotherapy, psychoanalysis). Occasionally, a systemic approach is necessary. Although each facet of the treatment must be conducted by a specialist, one physician must supervise the overall strategy; this role falls naturally to the family doctor. 相似文献
60.
C Pêcheux JF Mouret A Dürr Y Agid J Feingold A Brice C Dodé JC Kaplan 《Canadian Metallurgical Quarterly》1995,32(5):399-400
The CAG expansion responsible for Huntington's disease (HD) is followed by an adjacent polymorphic CCG repeat region which may interfere with a PCR based diagnosis. We have sequenced this region in 52 unrelated HD patients, from both normal and HD chromosomes. Fifty percent of the normal alleles were (CCG)7(CCT)2, 48% (CCG)10(CCT)2, and 2% (CCG)7(CCT)3. In contrast (CCG)7(CCT)2 was found in 85% of the HD alleles which represents significant linkage disequilibrium with the HD mutation. 相似文献