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81.
Garrett Jones Tae Jin Lee Joshua Glass Grace Rountree Lane Ulrich Amy Estes Mary Sezer Wenbo Zhi Shruti Sharma Ashok Sharma 《International journal of molecular sciences》2022,23(4)
The tear film is a multi-layer fluid that covers the corneal and conjunctival epithelia of the eye and provides lubrication, nutrients, and protection from the outside environment. Tear fluid contains a high concentration of proteins and has thus been recognized as a potential source of biomarkers for ocular disorders due to its proximity to disease sites on the ocular surface and the non-invasive nature of its collection. This is particularly true in the case of dry eye disease, which directly impacts the tear film and its components. Proteomic analysis of tear fluid is challenging mainly due to the wide dynamic range of proteins and the small sample volumes. However, recent advancements in mass spectrometry have revolutionized the field of proteomics enabling unprecedented depth, speed, and accuracy, even with small sample volumes. In this study using the Orbitrap Fusion Tribrid mass spectrometer, we compared four different mass spectrometry workflows for the proteomic analysis of tear fluid collected via Schirmer strips. We were able to establish a method of in-strip protein digestion that identified >3000 proteins in human tear samples from 11 healthy subjects. Our method offers a significant improvement in the number of proteins identified compared to previously reported methods without pooling samples. 相似文献
82.
Lane A. Hemaspaandra Rahman Lavaee Curtis Menton 《Annals of Mathematics and Artificial Intelligence》2016,77(3-4):191-223
Schulze and ranked-pairs elections have received much attention recently, and the former has quickly become a quite widely used election system. For many cases these systems have been proven resistant to bribery, control, or manipulation, with ranked pairs being particularly praised for being NP-hard for all three of those. Nonetheless, the present paper shows that with respect to the number of candidates, Schulze and ranked-pairs elections are fixed-parameter tractable to bribe, control, and manipulate: we obtain uniform, polynomial-time algorithms whose running times’ degrees do not depend on the number of candidates. We also provide such algorithms for some weighted variants of these problems. 相似文献
83.
Wittmann Marc; Lovero Kathryn L.; Lane Scott D.; Paulus Martin P. 《Canadian Metallurgical Quarterly》2010,3(1):15
Neuroimaging studies on delay discounting tasks that use reward delays ranging from minutes to days have implicated the insula and striatum in the processing of intertemporal decisions. This study aimed at assessing whether these brain regions would also be involved in decision-making when subjects have to wait through the delays within the range of seconds. Using functional MRI in 13 healthy volunteers, we repeatedly presented monetary options with delays that differed within the range of multiple seconds. Using a region-of-interest approach, we found significant activation in the bilateral anterior insula and striatum when subjects chose either the immediate (smaller) or delayed (larger) option. In particular, insular activation was observed after the response and the delay, when the outcome of the immediate or the delayed choice was shown. Significantly greater activation was observed in the ventroanterior striatum while subjects chose the immediate, as opposed to the delayed, options and also after receiving the outcome of waiting through the longer delay option. The evidence presented here indicates that both the ventral striatum and the insula are involved in the processing of choosing delay options as well as the consequences of choices with delays in the seconds' range. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
84.
Gbor Erdlyi Lane A. Hemaspaandra Jrg Rothe Holger Spakowski 《Theoretical computer science》2009,410(38-40):3995-4000
We show that many NP-hard sets have heuristic polynomial-time algorithms with high probability weight of correctness with respect to generalizations of Procaccia and Rosenschein’s junta distributions. 相似文献
85.
G. Flint S. Lamont B. Lane H. Sarrazin L. Lim Darlene Rini C. Roben 《Fire Technology》2013,49(3):767-792
The knowledge in the field of structural fire engineering has been greatly advanced through assessment of a number of real fires (WTC, Torre Windsor, Broadgate, etc.) and, especially, by the Cardington series of full scale structural fire tests. This knowledge has been used to validate and verify the use of computational finite element models that have expanded the range of structures that can be investigated under severe fire exposure. This paper presents a selection of key lessons learned by the authors through the assessment of structures in fire for real commercial building projects. The key areas of sensitivity that have been encountered are described and a discussion of each point presented. The paper is aimed at describing potential weaknesses that have been observed in the commercial work of the authors, often driven by the requirements for efficient ambient structural design. The paper concludes with some suggested advice for structural engineers aimed at increasing the general robustness of building structures. This is based on designing out as far as possible in the ambient design of a structure the potential weaknesses identified in past project work. 相似文献
86.
Harding CM Lane RG 《Journal of the Optical Society of America. A, Optics, image science, and vision》2002,19(2):295-305
The paper describes a volumetric approach to depth estimation for robot navigation with use of only an approximately calibrated translating camera. Our approach is related to techniques for photo-realistic object reconstruction but with the emphasis on issues associated with navigation. The technique performs three-dimensional matching by a process of image interpolation and can adjust for errors in camera position. The reconstruction is achieved from a small angular range of scene views, and the technique is demonstrated to be insensitive to large errors in the camera positions. The ability to correct for more critical errors such as the camera orientation is shown to significantly improve the algorithm's performance. Our technique is demonstrated on real image sequences and compares favorably with techniques based on optical flow. 相似文献
87.
We describe a novel technique for deriving wave-front aberrations from two defocused intensity measurements. The intensity defines a probability density function, and the method is based on the evolution of the cumulative density function of the intensity with light propagation. In one dimension, the problem is easily solved with a histogram specification procedure, with a linear relationship between the wave-front slope and the difference in the abscissas of the histograms. In two dimensions, the method requires use of a Radon transform. Simulation results demonstrate that good reconstructions can be attained down to 100 photons in each detector. In addition, the method is insensitive to scintillation at the aperture. 相似文献
88.
In 3 previous experiments, high doses of alcohol, marijuana, and alprazolam acutely increased risky decision making by adult humans in a 2-choice (risky vs. nonrisky) laboratory task. In this study, a computational modeling analysis known as the expectancy valence model (J. R. Busemeyer & J. C. Stout, 2002) was applied to individual-participant data from these studies, for the highest administered dose of all 3 drugs and corresponding placebo doses, to determine changes in decision-making processes that may be uniquely engendered by each drug. The model includes 3 parameters: responsiveness to rewards and losses (valence or motivation); the rate of updating expectancies about the value of risky alternatives (learning/memory); and the consistency with which trial-by-trial choices match expected outcomes (sensitivity). Parameter estimates revealed 3 key outcomes: Alcohol increased responsiveness to risky rewards and decreased responsiveness to risky losses (motivation) but did not alter expectancy updating (learning/memory); both marijuana and alprazolam produced increases in risk taking that were related to learning/memory but not motivation; and alcohol and marijuana (but not alprazolam) produced more random response patterns that were less consistently related to expected outcomes on the 2 choices. No significant main effects of gender or dose by gender interactions were obtained, but 2 dose by gender interactions approached significance. These outcomes underscore the utility of using a computational modeling approach to deconstruct decision-making processes and thus better understand drug effects on risky decision making in humans. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
89.
A novel interferometric optical Fourier-transform processor is presented that calculates the complex-valued Fourier transform of an image at preselected points on the spatial-frequency plane. The Fourier spectrum of an arbitrary input image is interfered with that of a reference image in a common-path interferometer. Both the real and the imaginary parts of the complex-valued spectrum are determined. The source and the reference images are easily matched to guarantee good fringe visibility. At least six interferograms are postprocessed to extract the real and the imaginary parts of the Fourier spectrum at preselected points. The proposed hybrid optical-digital technique is computationally appropriate when the number of desired spatial frequencies is small compared with the number of pixels in the image. When the number of desired points is comparable with the number of image pixels, a conventional or pruned two-dimensional fast Fourier transform is more appropriate. The number of digital operations required by the hybrid optical-digital Fourier processor is proportional to the number of desired spatial frequencies rather than the number of pixels in the image. The points may be regularly distributed over the spatial-frequency plane or concentrated in one or several irregularly shaped regions of interest. The interferometric optical Fourier processor is demonstrated in a moving-object trajectory estimation system. The system successfully estimates the trajectory of multiple objects moving over both stationary and white-noise backgrounds. A comparison of performance was made with all-digital computation. With everything else equal, our hybrid optical-digital calculation was more than 3 orders of magnitude faster. 相似文献
90.
A series of blends has been prepared by adding a polyetherimide (PEI), in varying proportions, to a tetrafunctional epoxy resin, tetraglycidyl-diaminodiphenylmethane (TGDDM), cured with 4,4′-diaminodiphenylsulphone (DDS). All the materials exhibited two-phase morphologies which were characterised by dynamic mechanical thermal analysis and scanning electron microscopy. Addition of the thermoplastic gave a modest improvement in fracture properties (K1c and G1c), as determined by three-point bending experiments, although no obvious correlation with the marked morphological changes was observed. 相似文献