Experimental and numerical investigation of the crushing response of an open section composite energy absorbing element

The quasi-static crushing response of carbon epoxy composite hat-shaped crush elements is described herein. A steeple-type triggering mechanism was used to ensure the specimens exhibited a continuous stable crushing mode of failure. The explicit finite element software PAM-CRASH was used to predict the crushing failure of these energy absorbing elements. A four-layer, stacked-shell model of the composite hat-shaped element, after calibration against experimental test data, was found to be capable of closely approximating the failure modes and provide agreement with the load vs. displacement behaviour observed during the experiments. The predicted steady state load and specific energy absorption were respectively within 1.5 and 0.2% of the experimental average. With further validation, the developed stacked-shell methodology could help provide a predictive tool to characterise the energy absorption of open section crush elements and significantly reduce the cost associated with an extensive experimental material characterisation test program.     

Two Subtle Amino Acid Changes in a Transaminase Substantially Enhance or Invert Enantiopreference in Cascade Syntheses

Amine transaminases (ATAs) are powerful enzymes for the stereospecific production of chiral amines. However, the synthesis of amines incorporating more than one stereocenter is still a challenge. We developed a cascade synthesis to access optically active 3‐alkyl‐substituted chiral amines by combining two asymmetric synthesis steps catalyzed by an enoate reductase and ATAs. The ATA wild type from Vibrio fluvialis showed only modest enantioselectivity (14 % de) in the amination of (S)‐3‐methylcyclohexanone, the product of the enoate‐reductase‐catalyzed reaction step. However, by protein engineering we created two variants with substantially improved diastereoselectivities: variant Leu56Val exhibited a higher R selectivity (66 % de) whereas the Leu56Ile substitution caused a switch in enantiopreference to furnish the S‐configured diastereomer (70 % de). Addition of 30 % DMSO further improved the selectivity and facilitated the synthesis of (1R,3S)‐1‐amino‐3‐methylcyclohexane with 89 % de at 87 % conversion.     

Mobile technology dominates school children’s IT use in an advantaged school community and is associated with musculoskeletal and visual symptoms

This paper describes the contemporary use of information technology devices by children in a socio-educationally advantaged school. A sample of 924 children (50% girls) from grades 5 to 12 (ages 10–19 years) completed an online survey in class. Total daily technology use was high and similar for girls (mean 219 (SD 148) mins/day) and boys (207 (142), p=.186). Tablet computer was the dominant device used in grades 5–9, with laptop computer the dominant device in grades 10–12. Patterns of exposure were influenced by gender, device, grade and purpose of use interactions. For example, girls used mobile phones more than boys for social purposes for grades 10 and 11, but not grade 12. Whilst children’s attitudes to technology use were positive, musculoskeletal and visual symptoms were commonly reported. Hours/day tablet and phone use was related to neck/shoulder discomfort (OR = 1.07; 1.13) and visual symptoms (OR = 1.10; 1.07).

Practitioner Summary: Technology use by children appears to be quite different now to a decade ago. This paper describes contemporary school children’s use of various devices for various purposes. The survey of >900 children found high technology use, dominated by new mobile technologies, and associations with musculoskeletal and visual symptoms.     

Self-multiplexing force-sense test structures for (MOS) ICapplications

A self-multiplexing technique that enables multiple force-sense measurements to be carried out on test structures in the small area scribe lanes of product chips is presented. Instead of using separate address signals, the technique uses the distribution of stimulus and sense voltages across lines to select the proper test structure, leading to a drastic reduction in the number of probe pads. A simple, robust prototype circuit which enables four independent test structures to be measured with only five probe pads is presented. The technique can be extended to 12 structures with some additional circuitry. Excellent measurement accuracy is obtained and the self-multiplexing circuit allows a wide range of operating conditions while being insensitive to process variations     

Shifting behavior: an analysis of response patterns of Parkinson patients in discrimination learning

Parkinson patients often show decreased performance on what is generally referred to as "shift tasks." This does not necessarily imply that Parkinson patients have problems with shifting, since task performance reflects not only shifting but also other factors. Using a discrimination learning task, we analyzed response patterns to determine the decision rules used. As well, we varied the manner of problem alternation (implicit versus explicit) and the type of problem alternation (extradimensional versus intradimensional shifts). In accordance with the literature, we found that Parkinson patients needed more trials to solve the problems. However, the response patterns of the Parkinson patients and controls were practically the same. An important finding was that Parkinson patients did not hold on longer to a rule, which was correct in a former problem, than controls did. Therefore, we concluded that Parkinson patients are able to shift from one decision rule to another.     

A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples

A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45+/-0.5 degrees C in lauryl sulfate tryptose broth supplemented with 0.5 M NaCl and 10 mg/liter vancomycin (mLST) for 22 to 24 h followed by streaking on tryptone soy agar with bile salts. When exposed to light during incubation at 37 degrees C, E. sakazakii produces yellow colonies within 24 h; identification was confirmed by testing for alpha-glucosidase activity and by using API 20E strips. All of the E. sakazakii strains tested (n = 99) were able to grow in mLST at 45+/-0.5 degrees C, whereas 35 of 39 strains of potential competitors, all belonging to the Enterobacteriaceae, were suppressed. A survey was carried out with 192 environmental samples from four different milk powder factories. Using this new protocol, E. sakazakii was isolated from almost 40% of the samples, whereas the reference procedure (enrichment in buffered peptone water, isolation on violet red bile glucose agar, and biochemical identification of randomly chosen colonies) only yielded 26% positive results. This selective method can be very useful for the rapid and reliable detection of E. sakazakii in environmental samples.     

Synergistic protection against cartilage destruction by low dose prednisolone and interleukin-10 in established murine collagen arthritis

BACKGROUND: For simplification of blood cell transplantation, an automated apheresis system that exploits a dual-stage channel device for mononuclear cell (MNC) collection (AutoPBSC, designed for the COBE Spectra) was studied. STUDY DESIGN AND METHODS: The automated default software (AutoPBSC-Default) and three software modifications of the harvest frequency during leukapheresis, referred to as AutoPBSC-1.25, AutoPBSC-1.75, and AutoPBSC-2.75, were evaluated in comparison with the semiautomated Version 4.7 (V4.7) apheresis system in 119 leukapheresis procedures performed in 90 cancer patients treated with chemotherapy plus granulocyte-colony-stimulating factor. CD34+ cell and platelet collection efficiency (CE); volume and cell composition of the leukapheresis components; and patient platelet and red cell (RBC) loss during leukapheresis were measured. RESULTS: The majority of collection measures evaluated with the AutoPBSC compared favorably to those obtained with the V4.7. CD34+ cell CE increased from 55 percent with V4.7 to 68 percent with the AutoPBSC-Default (p = 0.05). The AutoPBSC provided lower platelet contamination in the collected component (1.18 x 10(11) vs. 2.26 x 10(11) with the V4.7; p<0.001). The volume of the AutoPBSC-Default component was significantly lower (67 vs. 180 mL with the V4.7; p<0.001). The MNC purity of the AutoPBSC component was greater (52 vs. 28% with the V4.7; p<0.001), and the RBC contamination lower (AutoPBSC, 0.53 x 10(11) vs. 1.04 x 10(11) with the V4.7; p<0.001). Modifications of the AutoPBSC to increase the harvest frequency by 1.25-, 1.75-, and 2.75-fold resulted in increased CD34+ cell CE (77%, 75%, and 83%, respectively; p<0.001 in all cases), but also in reduced numbers of circulating platelets, higher platelet contamination of the component, and lower MNC purity than were seen with the AutoPBSC-Default. CONCLUSION:The AutoPBSC offers the following advantages over the V4.7 system: a) better CE of CD34+ cells; b) reduced collection of platelets; c) reduced contamination of the leukapheresis component with granulocytes, platelets, and RBCs; d) reduced component volume; and e) automation.