Langmuir films from antibodies based on amphiphilic polyelectrolytes

A technique for forming Langmuir films from antibodies based on an amphiphilic polyelectrolyte was developed. The physicochemical and immunochemical properties of the Langmuir films obtained were studied. The interaction of HBsAg with the films was found to be described by a model with one binding site, whereas that of HBsAg with antibodies adsorbed on a polystyrene plate, by a model with a positive cooperativity. The use of the novel Langmuir films from antibodies increases the sensitivity of the immunoenzyme assay.     

五号桩—长堤地区沙河街组砂体储集性及控制因素研究

沙河街组是沾化凹陷东部五号桩-长堤地区重要的储油层系，主要目的层段为沙三段和沙二段。沙三段和沙二段主要发育有浊积扇、水下冲积扇和滩坝沉积体系，在浊积扇、水下冲积扇中发育辫状水道、扇中前缘、辫状水道间以及扇根、扇缘等沉积微相类型，其中辫状水道、扇中前缘砂体物性好，原生孔隙及次生孔隙均较发育，辫状水道间以及扇根砂体物性较差。其储集特征主要受岩性、沉积微相、成岩作用等因素控制，结合该地区实际情况，将沙二段、沙三段储集层划分为好、中、差3种类型。图4表1参7     

CONSTITUTE AND PROPERTY OFα-Ti AFTER HYDRIDING TREATMENT

研究了氢化处理对退火α-Ti 板材的组织、结构及性能的影响。α-Ti 在室温出现两种结构的氢化物:有序的fctγ相(TiH)和fccδ相(TiH_2)。分析了用X 射线衍射只查觉到δ相的原因。氢化物的数量及形态影响除氢后基体的晶粒组织。控制氢含量可以改善拉伸性能及疲劳性能。     

傅立叶变换在虚拟仪器LabVIE中的应用

文中介绍了LabVIEW环境下用NI公司高级分析库里的信号处理工具包的Real FFT.vi傅立叶变换,采用此种方法对某型飞机起落架液压测试系统采样信号进行频谱分析,实验证明其效果良好.     

适用于稠油开采的游梁式抽油机设计分析

在稠油开采中,抽油杆在液体中运动时,所受到的摩擦阻尼力很大,有时高达几十千牛。上冲程时,该力有加大悬点载荷的作用;下冲程时,该力使抽油杆下行困难,甚至悬点载荷为零。为了开采稠油,必须根据其特点设计适应的游梁式抽油机。文章分析了稠油用抽油机的受力特点,提出了该类抽油机的设计要求。     

Control of Titanium Alloy Thin Plate Welding Distortion by Trailing Peening

The technology of trailing peening is a kind of promisingly bran-new technology which can be used to control weldingstress and distortion of Ti alloy thin plate. Control of TC4 Ti alloy thn plate welding stress and distortion underthe condition of convent     

艺术建筑设计的探索

通过徐州一中艺术楼的设计实践，寻求优美而独特的艺术形象，并用纯几何形体的组合来达到功能与形式的完美统一。     

Phenotypic analysis of antigen-specific T lymphocytes

Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.     

Antibody- and cell-mediated immune responses of Actinobacillus pleuropneumoniae-infected and bacterin-vaccinated pigs

The number involved in and the rate of migration of donor leucocytes into the following recipient organs (spleen, thymus, bone marrow, lung and mesenteric lymph nodes) were measured in two rat models of orthotopic liver transplantation (OLT) using donor-specific MHC class I antibodies. The first OLT model is one that does not require immunosuppression in order to achieve tolerance and involved the transplantation of DA (MHC haplotype, RT1a) livers into PVG (RT1c) recipients. The second model was one that required a 7-day (10 mg/kg) treatment with cyclosporin A (CsA) to achieve tolerance and used DA donors into Lewis (RT1(1)) recipients. Recipient organs were biopsied on days 3, 20 and 87 following OLT and donor leucocyte migration was quantified by immunohistochemistry and computer densitometry of immunoblots of detergent-solublized tissues in order to resolve both membrane-bound and soluble donor MHC class I antigen. In a separate experiment, spleen biopsies were taken following OLT on days 3 and 15 from the naturally tolerizing OLT model (DA into PVG), treated with and without CsA for 7 days and compared with the (DA into Lewis) model. The initial rate of leucocyte migration between days 3 and 21 following OLT was found to be the most rapid into the spleen, followed by the bone marrow and mesenteric lymph nodes in the naturally tolerant (DA into PVG) model when compared with the (DA into Lewis) model. The number of donor leucocytes in the spleen and mesenteric lymph nodes in both models was, however, approximately the same by 87 days. No real difference in the rate of leucocyte migration was seen in the thymus or the lung for both transplant models over the time course assayed. CsA was found to lower the rate of donor leucocyte migration only over the period it was administered. The rate of donor leucocyte migration into the spleen was still much lower 15 days after OLT in the (DA into Lewis) model compared with the (DA into PVG) model treated with and without CsA. Thus the differences in the rate of donor leucocyte migration into the spleen, bone marrow and mesenteric lymph nodes immediately following OLT may offer an explanation as to why the (DA into PVG) combination is able to accept a transplanted liver without immunosuppressive therapy.     

耐热钢主蒸汽管的焊接

1前言我厂动力区三期锅炉项目为2台75 t/h和1台130 t/h锅炉,与之配套的主蒸汽管道大量使用不同规格的15CrMo耐热管材,其主要规格为D273mm×11mm、D219mm×10mm、D159mm×9mm、D76mm×6mm、D36mm×4mm,管内蒸汽工作温度为450℃,工作压力为3.82 MPa,根据DL5007—92《电力建设施工及验收技术规范(》火力发电厂焊接篇)的要求,50%探伤,焊后24 h进行X射线探伤,评定标准为SD143—85,Ⅱ级合格。为保证质量,对15CrMo钢的焊接工艺特点进行了分析,评定了焊接工艺。2焊接工艺特点2、1 15CrMo属耐热钢,一般在热处理状态下焊接,其热处理状态为900…