Identification and cloning of the membrane-associated serine protease, hepsin, from mouse preimplantation embryos

Previous studies have suggested the existence of a membrane-associated serine protease expressed by mammalian preimplantation embryos. In this study, we have identified hepsin, a type II transmembrane serine protease, in early mouse blastocysts. Mouse hepsin was highly homologous to the previously identified human and rat cDNAs. Two isoforms, differing in their cytoplasmic domains, were detected. The tissue distribution of mouse hepsin was similar to that seen in humans, with prominent expression in liver and kidney. In mouse embryos, hepsin expression was observed in the two-cell stage, reached a maximal level at the early blastocyst stage, and decreased subsequent to blastocyst hatching. Expression of a soluble form of hepsin revealed its ability to autoactivate in a concentration-dependent manner. Catalytically inactive soluble hepsin was unable to autoactivate. These results suggest that hepsin may be the first serine protease expressed during mammalian development, making its ability to autoactivate critical to its function.     

Electromagnetic shower development in uranium and tungsten: A comparison of data from a silicon sampling calorimeter

Longitudinal electromagnetic shower development has been studied in uranium and tungsten using a sandwich calorimeter with silicon as the active medium. Data were taken with incoming electron energies of 2, 4 and 6 GeV. The silicon detectors were operated with depletion layers of both 200 and 70 μm. The energy resolution of the calorimeter was reduced by less than 10–15% when the detectors had depleted layer widths of 70 μm.     

Radiologic placement of long-term subcutaneous venous access ports in children

OBJECTIVE: The purpose of this study was to review the results of radiologically placed subcutaneous venous access ports in a pediatric population requiring long-term venous access. CONCLUSION: Sixteen of 20 patients had no significant problems with their ports. In the pediatric population, radiologically placed subcutaneous venous access ports offer a high success rate, a low complication rate, and significant cost savings over surgically placed ports.