复杂深井尾管固井及回接技术实践

尾管悬挂固井是一种工程经济效益较高、注水泥环空阻力较低且有利于改善套管柱轴向设计和再钻进水力条件的固井方法，常常应用于深井超深井的固井作业。鄯科1井是一口科学探索井，完井尾管具有井径极不规则（实测井径扩大率最大为139.82%，最小为－6.02%）、施工压力高（15~20 MPa）、封固段长（考虑水泥余量的情况下封固段可长达1500 m）和尾管回接固井要求封固两头且不使用分级注水泥等难点。文章针对固井难点，总结出一套适用于复杂深井尾管及其回接固井的优化设计关键技术，它主要包括管串选择、套管扶正器加法、水泥浆体系优选、施工压力校核和配套工艺措施。实践证明，这些技术对类似井的固井作业具有实际的参考价值。     

Stimulatory effect of follicle-stimulating hormone on basal and luteinizing hormone-stimulated testosterone secretions by the fetal rat testis in vitro

The in vitro effect of FSH on testosterone secretion by the fetal rat testis was studied. Testes were cultured in the presence or absence of either commercial human (h) FSH (Metrodine; 200 mIU/ml) or recombinant hFSH (200 mIU/ml) for 3 days and with 100 ng/ml ovine LH during the last 4 h of culture. To avoid a stimulatory effect by the 0.4% LH that contaminates Metrodine, the cultures were performed in the presence of a monoclonal anti-hLH beta antibody and with a concentration of Metrodine that had no short term stimulatory effect on testosterone production by the fetal testes in vitro. Metrodine treatment had a positive long term effect on both basal and LH-stimulated testosterone secretion by fetal testes explanted on days 18.5, 20.5, and 22.5 postconception, which was abolished by the addition of a monoclonal anti-hFSH beta antibody. LH-free recombinant FSH also augmented basal and LH-stimulated testosterone secretion of testes explanted on days 13.5, 14.5, and 18.5 postconception. The positive effect of recombinant hFSH appeared during the second day of treatment with day 14.5 and 18.5 testes and on the third day of treatment with day 13.5 testes. As it is widely accepted that FSH receptors are exclusively localized on Sertoli cells, these results suggest that on or before day 15.5 of fetal life, 1) Sertoli cells are able to respond to FSH, 2) Sertoli cells can produce factors that are able to act on Leydig cell function, and 3) Leydig cells are sensitive to FSH-induced Sertoli cell factors. In conclusion, this study points out a potential paracrine control of fetal Leydig cell function and/or differentiation by fetal Sertoli cells as soon as fetal Leydig cells differentiate.