Contribution of the C-terminal amino acid to the stability of Bacillus subtilis neutral protease

The role of the C-terminal Leu300 in maintaining thermal stabilityof the neutral protease of Bacillus subtilis was investigated.From model building studies based on the three dimensional structureof thermolysin, the neutral protease of B.thermoproteolyticus,it was conduded that this residue is located in a hydrophobicpocket composed of residues located in the C-terminal and themiddle domain. To test the hypothesis that Leu300, by contributingto a stabilizing interaction between these domains, is importantfor enzyme stability, several neutral protease mutants wereconstructed and characterized. The thermostability of the enzymewas lowered by deleting Leu300 or by replacing this residueby a smaller (Ala), a polar (Asn) or a sterically unfavourable(He) amino acid. Thermostabiity was increased upon replacingLeu300 by Phe. These results are in agreement with model-buildingstudies. The effects on thermostability observed after mutatingthe corresponding Val318 in the thermostable neutral proteaseof B.stearothermophilus were less pronounced.     

The impact of alkali elements on the degradation of CIGS solar cells

Unencapsulated CIGS solar cells with high and low contents of sodium (Na) and potassium (K) were simultaneously exposed to damp heat and illumination. The solar cells with a high alkali (Na, K) content exhibited higher initial conversion efficiencies, but degraded severely within 100 h, while the alkali poor samples kept relatively stable performance under damp heat and illumination. The degradation of the samples with a high alkali content resulted in the formation of sodium rich spots on the top ZnO:Al surface of the samples. This is likely caused by light‐induced Na⁺ migration via the grain boundaries in the absorber to the depletion region, where the Na⁺ accumulated. This allowed subsequent Na⁺ transport through the depletion region due to the lowering of the internal electric field caused both by the Na⁺ accumulation and illumination. The migration resulted in the formation of shunt paths, which reduced the shunt resistance and open circuit voltage. Furthermore, ingression of water into the ZnO:Al is expected to be responsible for a slow but steady increase in series resistance for both high and low alkali solar cells. Additionally, sodium migration led to a severe increase of the series resistance in case of alkali rich samples. Copyright © 2015 John Wiley & Sons, Ltd.     

Clockless Stochastic Decoding of Low-Density Parity-Check Codes: Architecture and Simulation Model

This paper introduces clockless stochastic decoding for high-throughput low-density parity-check (LDPC) decoders. Stochastic computation provides ultra-low-complexity hardware using simple logic gates. Clockless decoding eliminates global clocking, which eases the worst-case timing restrictions of synchronous stochastic decoders. The lack of synchronization might use outdated bits to update outputs in computation nodes; however, it does not significantly affect output probabilities. A timing model of clockless-computation behaviours under a 90 nm CMOS technology is used to simulate the BER performance of the proposed decoding scheme. Based on our models, the proposed decoding scheme significantly reduces error floors due to the “lock-up” problem and achieves superior BER performance compared with conventional synchronous stochastic decoders. The timing model includes metastability to verify the affect on BER performance.     

Suppression of phonon‐mediated hot carrier relaxation in type‐II InAs/AlAsxSb1 − x quantum wells: a practical route to hot carrier solar cells

InAs/AlAs_xSb_{1 − x} quantum wells are investigated for their potential as hot carrier solar cells. Continuous wave power and temperature‐dependent photoluminescence indicate a transition in the dominant hot carrier relaxation process from conventional phonon‐mediated carrier relaxation below 90 K to a regime where inhibited radiative recombination dominates the hot carrier relaxation at elevated temperatures. At temperatures below 90 K, photoluminescence measurements are consistent with type‐I quantum wells that exhibit hole localization associated with alloy/interface fluctuations. At elevated temperatures, hole delocalization reveals the true type‐II band alignment, where it is observed that inhibited radiative recombination due to the spatial separation of the charge carriers dominates hot carrier relaxation. This decoupling of phonon‐mediated relaxation results in robust hot carriers at higher temperatures, even at lower excitation powers. These results indicate type‐II quantum wells offer potential as practical hot carrier systems. Copyright © 2016 John Wiley & Sons, Ltd.     

Modified ESPRIT (M-ESPRIT) algorithm for time delay estimation in both any noise and any radar pulse context by a GPR radar

This paper presents M-ESPRIT, a modified version of the ESPRIT algorithm, for the purpose of time delay estimation of backscattered radar signals. The proposed algorithm takes both the transmitted pulse shape and any noise into account. It can process raw data from experimental device without the preprocessing which would be required with the conventional ESPRIT algorithm.     

Performance evaluation of path optimization schemes for inter‐switch handoff in wireless ATM networks

One of the major design issues in wireless ATM is the support of inter‐switch handoff. An inter‐switch handoff occurs when a mobile terminal moves to a new base station connecting to a different switch. Recently, a two‐phase handoff protocol has been proposed to support inter‐switch handoff in wireless ATM networks. With the aim of shortening handoff delay while using the network resources efficiently, the two‐phase handoff protocol employs path extension for each inter‐switch handoff, followed by path optimization if necessary. To implement the two‐phase handoff protocol efficiently, we need to determine when to trigger path optimization. In this paper, we propose and analyze three path optimization schemes, namely: periodic, exponential, and Bernoulli, for the two‐phase handoff protocol. The design objective is to determine the time to invoke path optimization such that the average cost per connection is minimized. We develop a discrete time analytical model and a discrete‐event simulation model for comparing the performance of the three path optimization schemes. Results indicate that the Bernoulli path optimization scheme outperforms the other two schemes by providing a lower average cost per connection. The proposed models can also be adapted to analyze other path optimization schemes in general. This revised version was published online in July 2006 with corrections to the Cover Date.     

DUV-induced micro and nanopatterning of polymer plasma deposited films

A simple method is described for patterning polymer surfaces with reactive groups. This entails pulsed plasma deposition of anhydride functionalized films, followed by DUV irradiation using a ArF excimer laser. Micro and nano-scale patterning was demonstrated, leading to well defined structures with controlled chemistry and/or geometries. We investigated the chemical changes induced by DUV irradiation. Among other parameters, we demonstrated that the covalent attachment of an amine terminated nucleophile via the aminolysis improved significantly the DUV photosensitivity. Using this approach it was possible to create combinatorial patterned surfaces. In particular such patterned polymer films appear as excellent candidate to study the effect of nanostructuration on the development of biofilms.     

Satellite image compression by concurrent representations of wavelet blocks

This paper proposes a complete compression and coding scheme for on-board satellite applications considering the main on-board constraints: low computational power and easy bit rate control. The proposed coding scheme improves the performance of the current Consultative Committee for Space Data Systems (CCSDS) recommendation for a low additional complexity. We consider post-transforms in the wavelet domain, select the best representation for each block of wavelet coefficients, and encode it into an embedded bit stream. After applying a classical wavelet transform of the image, several concurrent representations of blocks of wavelet coefficients are generated. The best representations are then selected according to a rate-distortion criterion. Finally, a specific bit-plane encoder derived from the CCSDS recommendation produces an embedded bit stream ensuring the easy rate control required. In this article, both the post-transforms and the best representation selection have been adapted to the low complexity constraint, and the CCSDS coder has been modified to compress post-transformed representations.