Resonance Raman spectra of iron(III)-, copper(II)-, cobalt(III)-, and manganese(III)-transferrins and of bis(2,4,6-trichlorophenolato)diimidazolecopper(II) monohydrate, a possible model for copper(II) binding to transferrins

Fe(III), Cu(II), Co(III), and Mn(III) complexes of ovo- and human serum transferrins show resonance enhanced Raman bands near 1600, 1500, 1270, and 1170 cm-1 upon excitation with laser frequencies which fall within the visible absorption bands of those metalloproteins. Comparison of the visible absorption and resonance Raman spectra of the Cu(II)-transferrin complexes with those for the Cu(II) model compound, bis(2,4,6-trichlorophenolato)diimidazolecopper(II) monohydrate, indicates that the resonance Raman bands are due to enhancement of phenolic vibrational modes. For the model (Cu(II) compound, a normal coordinate analysis was used to aid our assignment of the observed resonance bands at 1562, 1463, 1311, and 1122 cm-1 to A1 vibrational modes of the 2,4,6-trichlorophenolato moiety. These assignments are consistent with those made for Cu(II)-transferrins. The latter assignments were based upon calculated A1 frequencies for p-methylphenol (Cummings, D.L., and Wood, J.L. (1974), J. Mol. Struct. 20, 1). The wavelength shifts in the resonance bands for the model compound from those for Cu(II)-transferrins are due to the influence of the chloro substituents on the planar vibrations of phenol. These results clearly identify tyrosine as a ligand in copper binding to transferrins.     

An electrophoretic analysis of RNA synthesis in normal and lobeless Ilyanassa embryo

A primary infection with schistosoma mansoni, of 5 or more weeks duration, stimulated a high level of resistance to a challenge infection in the WO outbred strain of golden hamster. In sharp contrast, the LGN strain showed no statistically significant immunity to reinfection in most experiments and where detected the level of resistance was only about half that observed in the WO strain. Immunity to reinfection was assayed with the lung recovery method which assesses resistance a few days after challenge and with the conventional perfusion assay which measures immunity 6 weeks after challenge. Wide differences in the immune response to S. mansoni in different strains of the same host species have not been reported previously.     

Mechanism of Creep in MgO Brick

Creep of selected brands of periclase refractories was studied using rate process theory to provide information on activation energy and on the dominant method of deformation. A brand studied over the range 2200° to 2600°F at 25 psi gave an activation energy of 62 kcal/mole. All brands were studied at selected stresses and 2500°F to determine the flow mechanism. Plastic flow was indicated for all brands tested.     

AN EXPLORATORY STUDY OF A COMBINED SONIC AGGLOMERATION AND CROSSFLOW FILTRATION SYSTEM FOR HOT GAS CLEANUP

The Institute or Gas Technology has investigated a combined sonic agglomeration/crossflow filtration system to remove particles smaller than 10 microns from high-temperature, high-pressure gas streams. Sonic energy induces agglomeration so that particles can be removed in a continuously operating cross-flow filler element. Cold-model and preliminary high-temperature, high-pressure results are promising.

The objective of this investigation was to explore the potential effectiveness of sonic agglomeration, crossflow filtration, and a combination of these techniques to remove particles from high-temperature, high-pressure (HTHP) gas streams. The technique of sonic agglomeration has been known since the 1930's, and crossflow filtration has been used successfully in liquid filtration. This investigation is unique in that these two techniques were combined. Sonic energy was used to agglomerate particles to sizes large enough to be separated from the gas stream in a crossflow filter. The crossflow filter has advantages over conventional filters as a paniculate agglomerate removal system because it (1) operates continuously, (2) does not subject the fragile agglomerates to the high stress typical of inertial capture devices, and (3) can control the buildup of a filter cake when properly combined with a sonic agglomerator.

This investigation was supported by the Gas Research Institute and the Institute of Gas Technology Internal Research and Development Fund.

In this preliminary study, we found that—

• A 2-micron porosity filter must be used to achieve 98% paniculate removal from 95% of a dust-laden stream. (In the crossflow operating mode, 5% of the stream bypasses the filter.)

• When sonic agglomeration is combined with crossflow filtration, the same removal efficiency can be achieved with a 10-micron porosity filter.

• Combined sonic agglomeration/crossflow filtration removed particulates smaller than 10 microns in experiments at 265° C and 7000 kPa.

• The pressure drop across a 10-micron filter is about one-half that of a 2-micron filter, which could reduce the energy requirements for filtration.

This method of particulate removal should be applicable to many different coal reactor effluent streams, especially because it can operate at elevated temperatures and avoid gas cooling, liquid condensation, and subsequent liquid-solids separation. Preliminary estimates show that the power requirements of a combined sonic agglomerator/crossflow filter are lower than those of a crossflow filter alone, and that they are lower than or comparable with other particulate removal techniques. Additional tests are needed to establish the degree to which these benefits can be realized.