APPLICATION OF ATTRITION-MILLED SOY FLOUR IN A BEVERAGE APPLICATION

A soy beverage mix was created using thermally treated, attrition-milled soy powder, anticaking agent (1.0, 1.5 and 2.0%) and adjunct ingredients (salt, sugar, carrageenan, calcium phosphate) commonly found in a soy beverage. Thermal processing of defatted soy flake at specific moisture levels (30, 35 and 40%) from two different sources was evaluated for effective denaturation of the soy trypsin inhibitor complexes. Identification of an optimal thermal treatment was then used to produce a soy flake milled in a cyclone-assisted attrition mill. Soy beverage mixes were homogenized with a handheld blender at solution temperatures of 4, 30, and 70C. Separation rates were highest at all treatment levels consisting of the 70C solution temperature (1.67 mL/min) and were lowest for all treatment levels at 4C (0.55 mL/min). Thermal treatment of defatted soy flake resulted in solutions with lower viscosities (87.32 and 151.90 cP for Archer Daniels Midland Co. and L-Star, respectively).

PRACTICAL APPLICATIONS

This article establishes the milling conditions to create an instant soy beverage system. The soy drink mix can be easily used by consumers to make soy beverage or soymilk and is stable for up to 10 min.     

Bovine longissimus muscle tenderness as affected by postmortem aging time, animal age and sex

Strip loins were removed from 10 bulls and 10 steers ～14 mo old and from 10 cows ≥ 44 mo. Samples were removed at 3, 7, 14, and 28 days postmortem. Shear-force values were measured. Sensory evaluation was done by a 15-member trained panel. Animal age and postmortem aging time had more influence on tenderness attributes than did sex of the animal. Increased postmortem aging time improved tenderness attributes regardless of sex or age.     

Protein Quality of Selected Muscle Foods as Affected by the Exchange of Dietary Wheat Bran for Cellulose

The exchange of 8.4% neutral detergent fiber (NDF) of wheat bran for 8.3% NDF of cellulose in diets containing either beef, chicken, fish or casein as protein sources did not (P>0.05) alter feed consumption, weight gain or energy intake in the protein efficiency ratio (PER) bioassay with weanling rats. Higher (P<0.05) fecal nitrogen (N) excretions and lower (P<0.05) N digestibilities occurred for each protein source when wheat bran was exchanged for cellulose. The exchange also had no effect (P>0.05) on PER or net protein ratio (NPR) of casein or fish protein or on NPR of chicken protein. However, PER and NPR of beef and PER for chicken were reduced (P<0.05) when the dietary fiber was exchanged.     

Lactic Acid Concentration and Microbial Spoilage in Anaerobically and Aerobically Stored Ground Beef

Lactic acid concentration of coarsely ground beef, packaged in low oxygen permeability casings, is inversely related to sensory acceptability of the meat following regrinding and aerobic storage for six additional days. Samples, reground and stored aerobically after periods of anaerobic storage, were assayed for total aerobic bacterial counts, percentage of gram-positive microflora, and pH. Lactic acid concentration ranged from 130 to 1055 mg per 100g ground beef and increased with anaerobic storage. During 6 days of aerobic storage, both increases and decreases in concentration were noted and related to changes in microbial flora. A positive correlation was found between the proportion of gram-positive bacteria (lactic acid bacteria) and lactic acid and pH.     

CHARACTERIZATION OF STOMACH AND PYLORIC CAECA PROTEINASES OF TAMBAQUI (COLOSSOMA MACROPOMUM)

The effects of pH, temperature, inhibitors, and substrates on proteolytic activities of crude extracts from the stomach, liver, pyloric caeca, and intestines of Colossoma macropomum were investigated. The highest acid and alkaline proteolytic activities were found in stomach and pyloric caeca, respectively. The optimum pH for the acid and alkaline proteases were 1.8 and 7.0–9.0, respectively, while the optimum temperatures were 35C and 65C. This alkaline protease thermal stability remained unaltered after 90 min incubation at 55C. A pepsin‐like protease was responsible for most of the acidic proteolytic activity (Pepstatin A inhibited approximately 90%), whereas PMSF inhibited about 40% of the alkaline protease. The alkaline proteolytic activity has attractive thermal properties for industrial applications.     

Variations in Radiation Sensitivity of Foodborne Pathogens Associated with the Suspending Meat

Longissimus dorsi from beef, pork, and lamb and turkey breast and leg meats were inoculated with Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus, and the gamma radiation resistance of the pathogens were determined under identical conditions. At 5°C the respective radiation D-values of E. coli O157:H7 and L. monocytogenes did not vary with the suspending meat. The D-value for a mixture of Salmonella spp. was significantly lower on pork than on beef, lamb, turkey breast, and turkey leg meats. The D-value for S. aureus was significantly lower on lamb and mechanically deboned chicken meat than on the other meats. All values were, nevertheless, within expected ranges.     

MULTI-RESIDUE DETERMINATION AND CONFIRMATION OF TEN HETEROCYCLIC AMINES IN COOKED MEATS

Heterocyclic aromatic amines (HAAs) have been identified in various cooked meat products. HAAs are formed from pyrolysis of proteins and amino acids and are of interest because many of these primary arylamines are mutagens and rodent carcinogens. We developed a screening method for ten HAAs using liquid-liquid extraction, reverse-phase HPLC separation, and tandem electrochemical (ECD) and fluorescence (FLU) detectors. This detection system exhibited greater sensitivity (50-100 fold) for HAAs than the HPLC-UV methods. HPLC-FLU has detection limits of 100 pg to 1 ng for six HAAs, and HPLC-ECD detection limits of four HAAs were 250 pg to 1 ng. We also developed a confirmatory procedure for ten HAAs using HPLC coupled to positive ion APCI-MS. This technique has subnanogram detection limits. These methods were used to quantify HAAs in meat products at levels formed by typical Western cooking methods.     

In-use measurement of activity, energy use, and emissions of a plug-in hybrid electric vehicle

Plug-in hybrid electric vehicles (PHEVs) could reduce transportation air emissions and energy use. However, a method is needed for estimating on-road emissions of PHEVs. To develop a framework for quantifying microscale energy use and emissions (EU&E), measurements were conducted on a Toyota Prius retrofitted with a plug-in battery system on eight routes. Measurements were made using the following: (1) a data logger for the hybrid control system; (2) a portable emissions measurement system; and (3) a global positioning system with barometric altimeter. Trends in EU&E are estimated based on vehicle specific power. Energy economy is quantified based on gasoline consumed by the engine and grid energy consumed by the plug-in battery. Emissions from electricity consumption are estimated based on the power generation mix. Fuel use is approximately 30% lower during plug-in battery use. Grid emissions were higher for CO?, NO(x), SO?, and PM compared to tailpipe emissions but lower for CO and hydrocarbons. EU&E depends on engine and plug-in battery operation. The use of two energy sources must be addressed in characterizing fuel economy; overall energy economy is 11% lower if including grid energy use than accounting only for fuel consumption.     

Development of an epitope-specific analytical tool for the major peanut allergen Ara h 2 using a high-density multiple-antigenic peptide strategy

Using the major peanut allergen Ara h 2 as an example, an analytical tool enabling the determination of immunoglobulin E (IgE)-epitopes in processed food allergens was developed. We synthesized a multiple-antigenic peptide (MAP) of the IgE-reactive linear epitope 3 (amino acid positions 27-36) of Ara h 2 and raised a monospecific antiserum against this epitope to obtain a positive control for future epitope resolved diagnostics. First, a MAP of epitope 3, having a molecular mass of 7770 Da, was synthesized, purified, and its structure confirmed by liquid chromatography-mass spectrometry (electrospray ionization) (LC-MS(ESI)), matrix assisted laser desorption/ionization-time of flight (MALDI-TOF), and Edman sequencing. The MAP was then used to raise high titer antibodies in rabbits using the adjuvant Titermax and to characterize the specificity of IgE from allergenic patients sensitized to Ara h 2. The antiserum exclusively detects Ara h 2 in crude peanut extract with a titer of 10(7) by Western blot and reacts specifically with epitope 3 shown by epitope mapping for a library of solid-phase-bound synthetic 15-mer peptides covering the entire sequence of Ara h 2. Such IgE-reactive epitopes are of high analytical relevance as they could constitute the basis for epitope-specific detection systems for use in quality control in the food industry or for forensic purposes in cases of fatal reactions to otherwise undetected peanut proteins.