Combined phase singularity and wavefront analysis for optical maps of ventricular fibrillation

Much of the research into the mechanisms of ventricular fibrillation (VF) employs high-resolution mapping of electrical activation and recovery patterns. We previously developed a method for analyzing electrically mapped VF patterns that was based on identifying individual VF wavefronts. We now introduce a related method designed to take into account the information on repolarization that is present in optically mapped VF data. The new method first converts raw fluorescence data to an angular variable that tracks the phase of the mapped tissue through the depolarization-repolarization cycle. We define wavefronts in this context as isolines of phase that terminate either at boundaries or at singular points within the phase field. These singularities are the pivots of functional reentry and are important determinants of VF patterns. We parameterize VF by constructing data structures that describe wavefronts and singularities and also maintain wavefront-wavefront, wavefront-singularity, and singularity-singularity relationships. We describe one important application of this parameterization, which is to identify, localize, and characterize the importance of occurrences of propagation block during VF.     

Dissolution Behaviors and Applications of Silicon Oxides and Nitrides in Transient Electronics

Silicon oxides and nitrides are key materials for dielectrics and encapsulation layers in a class of silicon‐based high performance electronics that has ability to completely dissolve in a controlled fashion with programmable rates, when submerged in bio‐fluids and/or relevant solutions. This type of technology, referred to as “transient electronics”, has potential applications in biomedical implants, environmental sensors, and other envisioned areas. The results presented here provide comprehensive studies of transient behaviors of thin films of silicon oxides and nitrides in diverse aqueous solutions at different pH scales and temperatures. The kinetics of hydrolysis of these materials depends not only on pH levels/ion concentrations of solutions and temperatures, but also on the morphology and chemistry of the films, as determined by the deposition methods and conditions. Encapsulation strategies with a combination of layers demonstrate enhancement of the lifetime of transient electronic devices, by reducing water/vapor permeation through the defects.     

A twin slot antenna on a layered substrate coupled to a microstrip feed line

A method is described for calculating the input impedance as seen by the detector for a twin slot antenna structure. This technique is then used to analyze several twin slot antenna configurations, leading to improved performance.     

Poles and Zeros – Examples of the Behavioral Approach Applied to Discrete Linear Repetitive Processes

In this paper the behavorial approach is applied to discrete linear repetitive processes, which are class of 2D systems of both systems theoretic and applications interest. The main results are on poles and zeros for these processes, which have exponential trajectory interpretations. Published online: April 2006     

Robust and accurate registration of 2-D electrophoresis gels using point-matching.

Point-matching is a widely applied image registration method and many algorithms have been developed. Registration of 2-D electrophoresis gels is an important problem in biological research that presents many of the technical difficulties that beset point-matching: large numbers of points with variable densities, large nonrigid transformations between point sets, paucity of structural information and large numbers of unmatchable points (outliers) in either set. In seeking the most suitable algorithm for gel registration we have evaluated a number of approaches for accuracy and robustness in the face of these difficulties. Using synthetic images we test combinations of three algorithm components: correspondence assignment, distance metrics and image transformation. We show that a version of the iterated closest point (ICP) algorithm using a non-Euclidean distance metric and a robust estimation of transform parameters provides best performance, equalling SoftAssign in the presence of moderate image distortion, and providing superior robustness against large distortions and high outlier proportions. From this evaluation we develop a gel registration algorithm based on robust ICP and a novel distance metric combining Euclidean, shape context and image-related features. We demonstrate the accuracy of gel matching using synthetic distortions of real gels and show that robust estimation of transform parameters using M-estimators can enforce inverse consistency, ensuring that matching results are independent of the order of the images.     

Automatic detection of conduction block based on time-frequencyanalysis of unipolar electrograms

It is commonly thought that lethal tachyarrhythmias, such as ventricular fibrillation (VF), are perpetuated by functional reentry, which occurs when an activation wave blocks and rotates around tissue that is excitable (i.e., functional block). Electrograms recorded near these regions typically contain two sequential deflections representing activation on either side of the block. By detecting these "double potentials," we hypothesize that functional block can be detected by a single electrode. METHODS: Unipolar electrograms were recorded from a 24 x 21 mapping array on the intact ventricular epicardium of five pigs during electrically-induced VF. The short time Fourier Transform (STFT) of each electrogram was analyzed to identify double potentials. To evaluate the performance of the STFT algorithm, conduction block was located in activation maps using a minimum conduction velocity criterion (10 cm/s) and then compared to the results of the STFT algorithm. RESULTS: The STFT algorithm detected conduction block with a sensitivity of 0.74 +/- 0.12 and a specificity of 0.99 +/- 0.00. CONCLUSION: We have developed an automated algorithm that can detect functional block during VF from a single electrode recording. Possible applications include fast, objective identification of block in mapping data and realtime localization of reentrant substrates using mapping catheters.     

A 5-GHz radio front-end with automatically Q-tuned notch filter and VCO

A low-voltage receiver front-end for 5-GHz radio applications is presented. The receiver consists of a low-noise amplifier (LNA) with notch filter, a voltage-controlled oscillator (VCO), and a mixer. The LNA/notch filter has an automatic Q-tuning circuit integrated with it to provide good image rejection. On-chip transformers are used extensively in the receiver to improve performance and facilitate low-voltage operation. The receiver has a gain of 19.8 dB, noise figure of 4.5 dB, a third-order input intercept point (IIP3) of -11.5 dBm, and an image rejection of 59 dB, and the VCO had a phase noise of -116 dBc/Hz at 1-MHz offset.     

Photolithographic Route to the Fabrication of Micro/Nanowires of III–V Semiconductors

Nano/microwires of semiconducting materials (e.g., GaAs and InP) with triangular cross‐sections can be fabricated by “top–down” approaches that combine lithography of high‐quality bulk wafers (using either traditional photolithography or phase‐shift optical lithography) with anisotropic chemical etching. This method gives good control over the lateral dimensions, lengths, and morphologies of free‐standing wires. The behaviors of many different resist layers and etching chemistries are presented. It is shown how wire arrays with highly ordered alignments can be transfer printed onto plastic substrates. This “top–down” approach provides a simple, effective, and versatile way of generating high‐quality single‐crystalline wires of various compound semiconductors. The resultant wires and wire arrays have potential applications in electronics, optics, optoelectronics, and sensing.     

Oxygen-Generating Cryogels Restore T Cell Mediated Cytotoxicity in Hypoxic Tumors

Solid tumors are protected from antitumor immune responses due to their hypoxic microenvironments. Weakening hypoxia-driven immunosuppression by hyperoxic breathing of 60% oxygen has shown to be effective in unleashing antitumor immune cells against solid tumors. However, efficacy of systemic oxygenation is limited against solid tumors outside of lungs and has been associated with unwanted side effects. As a result, it is essential to develop targeted oxygenation alternatives to weaken tumor hypoxia as novel approaches to restore immune responses against cancer. Herein, injectable oxygen-generating cryogels (O₂-cryogels) to reverse tumor-induced hypoxia are reported. These macroporous biomaterials are designed to locally deliver oxygen, inhibit the expression of hypoxia-inducible genes in hypoxic melanoma cells, and reduce the accumulation of immunosuppressive extracellular adenosine. The data show that O₂-cryogels enhance T cell-mediated secretion of cytotoxic proteins, restoring the killing ability of tumor-specific cytotoxic T lymphocytes, both in vitro and in vivo. In summary, O₂-cryogels provide a unique and safe platform to supply oxygen as a coadjuvant in hypoxic tumors and have the potential to improve cancer immunotherapies.