Early Expression of Tet1 and Tet2 in Mouse Zygotes Altered DNA Methylation Status and Affected Embryonic Development

Ten-eleven translocation (Tet) dioxygenases can induce DNA demethylation by catalyzing 5-methylcytosine(5mC) to 5-hydroxymethylcytosine(5hmC), and play important roles during mammalian development. In mouse, Tet1 and Tet2 are not expressed in pronucleus-staged embryos and are not involved in the genomic demethylation of early zygotes. Here, we investigated the influence of Tet1 and Tet2 on methylation of parental genomes by ectopically expressing Tet1 and Tet2 in zygotes. Immunofluorescence staining showed a marked 5hmC increase in the maternal pronucleus after injection of Tet1 or Tet2 mRNA into zygotes. Whole-genome bisulfite sequencing further revealed that Tet2 greatly enhanced the global demethylation of both parental genomes, while Tet1 only promoted the paternal demethylation. Tet1 and Tet2 overexpression altered the DNA methylation across genomes, including various genic elements and germline-specific differently methylated regions. Tet2 exhibited overall stronger demethylation activity than Tet1. Either Tet1 or Tet2 overexpression impaired preimplantation embryonic development. These results demonstrated that early expression of Tet1 and Tet2 could substantially alter the zygotic methylation landscape and damage embryonic development. These findings provide new insights into understanding the function of Tet dioxygenases and the mechanism of DNA methylation in relation to embryogenesis.     

Synthesis of Sulfonated Carbon Nanocage and Its Performance as Solid Acid Catalyst

Abstract  

A carbon nanocage material (CKT) was first successfully sulfonated by introducing sulfophenyl groups on the surface of pore channels through benzenesulfonic acid-containing aryl radical in situ generated from the reaction of 4-aminobenzenesulfonic acid and isoamyl nitrite in water. The sulfonated carbon nanocage material (S-CKT) was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, powder small-angle X-ray diffraction and nitrogen sorption measurements. The results showed that the S-CKT still possess the high specific surface area (787 m²/g) and uniform mesoporous (pore diameter 4.7 nm) structures, although the structure of S-CKT is slightly disorder, compared with its unsulfonated precursor. S-CKT, as a carbon-based solid acid catalyst, showed good catalytic performance and reusability in the cross-Aldol condensation of ketones with aromatic aldehydes under solvent-free condition.     

我国蒸气压缩式空调机专利技术的发展现状与分析

对2000—2006年在我国申请以及授权的关于蒸气压缩式空调机四个主要部件——压缩机、冷凝器、蒸发器、膨胀阀的发明及实用新型专利的数据进行了检索及统计,分析了近年来我国在这一领域的发展水平,间接反映出不同地区及行业不同单位的科研能力。     

Ce对共晶Al-Si合金微观组织的影响

研究了Ce的变质处理时间和加入量对共晶Al-Si合金微观组织的影响。结果表明,Ce对共晶Al-Si合金有明显的变质效果,经Ce变质处理后,共晶Si由长针状变为珊瑚状;Ce的变质处理时间和加入量也会影响变质效果,Ce在共晶Al-Si合金中的最佳加入量(质量分数,下同)为1%,最佳变质处理时间为60~90min。     

补偿回弹的冲压件模具设计方法

回弹是金属板料冲压中的主要缺陷之一,它可以通过修正模具形状加以解决.基于对经过成形--回弹的有限元数值模拟,提出一种循环位移补偿回弹修正模具型面的方法.它基于对经过成形--回弹有限元数值模拟得到的成形工件与目标工件多次循环比较,用成形工件结点回弹位移修正实验模具形状,直到利用修正的模具能够换获得满意的工件为止.利用此方法对一小型三维铝合金板料冲压工件的冲压模具设计过程进行了数值模拟,结果表明,通过两次修正的模具可获得满意工件.并根据模拟得到的型面制作模具进行了实冲实验,证明此补偿回弹的模具修正法是有效的.     

浅析聚丙烯粉料中晶点的生成与预防

结合小本体聚丙烯的生产，对聚丙烯粉料中存在的晶点按生成原因从晶点料，高温料和碎料等不同的情况进行了分析，并提出预防措施。     

低熔点Cu-P基中间合金及其变质效果

利用差示扫描量热仪(DSC)、扫描电子显微镜(SEM)和金相显微镜等手段对Cu-P基中间合金进行了研究。结果表明，在二元共晶Cu-P中间合金中加入一定量的Sn可制成低熔点的Cu-16Sn-8P中间合金；在此基础上研制出一种熔点为630℃左右的低熔点的多元Cu-P基中间合金。与Cu-P中间合金相比，该低熔点多元Cu-P基中间合金对过共晶AI-24Si合金具有更好的变质效果。同时，该中间合金对ZL109合金也具有良好的变质效果。     

机床的静压系统及润滑

对机床的静压系统及其发展过程进行了综述 ,介绍了静压系统的基本结构、特点及其在机床中的各种运用。