Odourous algal-derived alkenes: differences in stability and treatment responses in drinking water.

Drinking water supplies are often impacted by taste and odour (T/O) episodes caused by algal volatile organic compounds (AVOCs) from algal blooms. Treatment and control of these events is important to utility operators, as customer confidence in the safety of public drinking water supplies is based primarily on their palatability and odour. To manage T/O outbreaks successfully, knowledge about treatment responses of AVOCs and anticipation of their outbreaks are thus of major importance to the water industry. The Glenmore Reservoir and water treatment plant (GWTP) supplies drinking water to over 50% of the ca. 1 million consumers in Calgary (Alberta). Despite low nutrients and high raw water quality, the reservoir experiences periodic outbreaks of fishy/floral T/O, caused by chrysophytes and diatoms (Uroglena americana, Dinobryon spp., Synura petersenii, Asterionella formosa). These odours are produced by the unsaturated C7-C10 alkenes 2,4-heptadienal, 2,4,7-octatriene, 2,4-decadienal and 2,4,7-decatrienal, generated during from the enzymatic breakdown of algal polyunsaturated fatty acids (PUFAs). The formation, persistence and stability of these compounds in both the raw water and treatment plant is not well understood.     

Exploring Particle Density Effects on Partial Mobility of Steelhead Spawning Gravels

Yellowbank Creek is a small stream in coastal central California being assessed for salmonid habitat limiting factors and restoration potential. Yellowbank flows through low‐density marine mudstone bedrock, which is the gravel source for the stream. To assess the potential effects of the low‐density substrate on spawning gravels, a tracer stone study comparing the incipient motion of low‐density mudstone particles and typical density granitic particles was used to populate a logistic regression particle entrainment model. A model comparison approach was used to test the strength of the model. Results demonstrate partial mobility of both mudstone and granitic particles under boundary shear conditions ranging from 6.9 to 42.2 N m^‐2. The modelling results quantify the strong negative correlation between particle entrainment and particle density. Mudstone gravel was three times more likely to be entrained than granitic gravel, within the context of the experimental conditions. The effect of density difference on partial mobility was greater in smaller grain size fractions. This work has implications for salmonid spawning success in atypical geologic settings and may assist in prioritization of restoration efforts. Copyright © 2013 John Wiley & Sons, Ltd.     

A cannabinoid with cardiovascular activity but no overt behavioral effects

Abnormal-delta8-tetrahydrocannabinol (ABN-delta8-THC) failed to elicit central nervous system and cardiovascular effects in laboratory animals. Abnormal-cannabidiol (ABN-CBD) was also devoid of overt behavioral effects but produced marked hypotension with only slight bradycardia in anesthesized dogs.     

Orientation of slit pupil and visual streak in the eye of the cat

The orientation of the visual streak of the cat's retina was compared to that of the long axis of the slit pupil in the same eye. In five paralyzed, anesthetized cats, the retinal projection to the superior colliculus was mapped with electrophysiological techniques. The orientation of the visual streak was estimated from the projection in visual space of the collicular region of high magnification which corresponds to the central projection of the streak. The angle by which the streak was tilted from absolute horizontal was always within one or two degree of the angle by which the pupil axis was tilted from absolute vertical. This relationship was confirmed in three of the animals in which small retinal lesions were placed a known distance from the histologically determined axis of the streak. From the visual coordinates of these lesions, an independent estimate of the streak's orientation was obtained. In each case, the tilt of streak axis from horizontal differed by no more than 0.5 degrees from the tilt of the pupil axis from vertical. The results support the hypothesis that planes containing the long axis of the cat's slit pupil are perpendicular to planes containing the long axis of the visual streak of the same eye.     

Failure of triiodothyronine to inhibit TSH-mediated thyroid hormone release in man

We studied the effect of short-term triiodothyronine administration on thyroid gland responsivity to exogenous thyrotropin in four euthyroid human subjects. Thyroidal iodine release and serum thyroxine during daily im injections of bovine TSH were not significantly inhibited, despite a four-fold elevation in serum T3 concentrations. This negative finding contrasts with earlier positive reports of a regulatory "short-loop" effect of elevated circulating T3 on the thyroid gland. This difference may be due either to the use in previous murine or in vitro studies of non-physiologic, high doses of exogenous T3, or failure to control the withdrawal of the trophic effect of endogenous TSH in man on the subsequent glandular response.     

Estimation of serum gentamicin by quenching fluoroimmunoassay

A new type of non-isotopic immunoassay, applied to the determination of serum gentamicin, is reported. The method is based on partial quenching of fluorescence observed when fluorescein-labelled gentamicin is bound by anti-gentamicin serum. The fluorescence intensity of the labelled gentamicin in an unseparated immunoassay incubation mixture therefore serves to indicate the extent of binding, which is related to the amount of competing unlabelled gentamicin present. Precision and accuracy are shown to be similar to those of the best existing methods for gentamicin, while the new assay is more rapid and technically simpler, and avoids the use of expensive radio-chemicals with their attendant health hazard. Assays of patient samples correlate with established bioassay and polarisation fluoroimmunoassay methods.     

Early prostaglandin release from the ischemic myocardium in the dog

Cellular consequences of myocardial ischemia were studied in anesthetized dogs. Confirmation of myocardial ischemia was provided by electrocardiographic and biochemical indexes. Prostaglandin F2alpha release into coronary venous blood was significantly elevated during myocardial ischemia, whereas indomethacin treatment prevented this increase in coronary venous prostaglandin F2alpha concentrations. No significant increase in prostaglandin E2 release was observed in response to myocardial ischemia, but indomethacin treatment significantly reduced coronary venous prostaglandin E2 concentrations below those of control values. Within one hour after occlusion of the coronary artery, the S-T segment was significantly altered, and coronary venous prostaglandin F2alpha had increased significantly above the control concentration. These changes persisted during four hours of myocardial ischemia. Plasma creatine phosphokinase activity increased significantly after two hours of myocardial ischemia and remained elevated for the subsequent two hours of ischemia. After four hours of myocardial ischemia, myocardial creatine phosphokinase activity of ischemic myocardium was significantly reduced, and labilization of myocardial treatment prevented increases in prostaglandin release but did not influence other biochemical changes or the electrocardiographic response to ischemia. Thus, prostaglandin release by ischemic myocardial tissue is an early response to the ischemic stimulus.     

Effects of exercise on fluid exchange and body composition in man during 14-day bed rest

To determine the cause of the body weight loss during bed rest (BR), fluid balance and anthropometric measurements were taken from seven men (19-21 yr) during three 2-wk BR periods which were separated by 3-wk ambulatory recovery periods. Caloric intake was 3,073 +/- 155 (SD) kcal/day. During two of the three BR periods they performed supine isotonic exercise at 68% of VO2max on the ergometer for 1 h/day; or supine isometric exercise at 21% of maximal leg extension force for 1 min followed by a 1-min rest for 1 h/day. No prescribed exercise was given during the other BR period. During BR, body weight decreased slightly with no exercise (-0.43 kg, NS), but decreased significantly (P less than 0.05) by -0.91 kg with isometric and by -1.77 kg with isotonic exercise. About one-third of the weight reduction with isotonic exercise was due to fat loss (-0.69 kg) and, the remainder, to loss of lean body mass (-0.98 kg). It is concluded that the reduction in body weight during bed rest has two major components: First, a loss of lean body mass caused by assumption of the horizontal body position that is independent of the metabolic rate. Second, a loss of body fat content that is proportional to the metabolic rate.     

Estimating channel‐forming discharge in urban watercourses

Several methods of estimating channel‐forming discharge were conducted on 12 quasi‐stable urban stream channels ranging from 9 to 99% urban land use to test their applicability in the urban condition. Bankfull stage was identified at a series of locations along each study reach and it was found that the most consistent observations of bankfull discharge occurred during flood conditions where bankfull stage was identified at the top of point bars along the convex arc of bends. The largest errors in estimation occurred at gauge stations where cross‐sectional geometry had been altered to conform to bridges or culverts rather than the channel morphology. Independent evaluations of channel forming discharge were conducted by 11 practitioners ranging from 10 years to 43 years of experience with similar findings and errors. Various methods of relating frequency return periods were evaluated using annual peak series discharge observations and continuous 15‐min systematic discharge records using partial duration series analysis. Bankfull discharge was observed to occur more than once a year in all of the urban streams studied and often averaged from 4 to 8 bankfull discharge or larger events per year. In one particular case in a single given year 18 events exceeding bankfull discharge were observed. No specific correlations were identified between frequency return periods and land use change. However, based upon the findings of this study, the applicability of employing annual series peak discharge data to evaluate bankfull frequency return in urban stream channels is highly discouraged. Copyright © 2010 John Wiley & Sons, Ltd.     

Simulation of the mechanical properties of an aluminum matrix composite using X-ray microtomography

Aluminum-based particulate-reinforced metal matrix composites (PMMCs) frequently have a heterogeneous distribution of reinforcement particles whether produced by a powder or liquid processing route. The applicability of X-ray microtomography (XMT) for the characterization of this heterogeneity, and its influence on final properties, was investigated for the case of a powder blended and extruded AA2124 matrix with Ni particulate. Three-dimensional image analysis techniques were used to quantify the embedded Ni particle size distribution and the extent and texture of clusters formed. The XMT data were exploited as a rapid method to generate a microstructurally accurate and robust three-dimensional mesh for input for finite-element modeling. Simulation of the elastoplastic response of the material showed excellent correlation with experimental results. This article is based on a presentation made in the symposium “Computational Aspects of Mechanical Properties of Materials,” which occurred at the 2005 TMS Annual Meeting, February 13–17, 2005, in San Francisco, CA, under the auspices of the MPMD-Computational Materials Science & Engineering (Jt. ASM-MSCTS) Committee.