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121.
JW Erdman AJ Thatcher NE Hofmann JD Lederman SS Block CM Lee S Mokady 《Canadian Metallurgical Quarterly》1998,128(11):2009-2013
The algae Dunaliella bardawil and Dunaliella salina naturally contain large concentrations of all-trans and 9-cis beta-carotene (betaC). The purpose of this study was to compare the relative serum and tissue accumulation of all-trans and 9-cis betaC in ferrets fed different ratios of all-trans/9-cis betaC derived from two commercial sources, D. bardawil or D. salina (Betatene). Male ferrets (7 wk old) were fed carotene-free, pelleted diets for 27 d. Beginning on d 18, groups of ferrets (n = 6 or 7) received daily, one of six oral supplements varying in ratios of 9-cis and all-trans betaC mixed with approximately 1.0mL of Ensure. Four supplements containing 5.2-8.3 micromol total betaC were prepared from a 20% Betatene preparation, D. bardawil, a high-cis Betatene preparation, and Betatene further enriched in 9-cis betaC with all-trans betaC/9-cis betaC ratios of 2.2, 1.5, 0.6 and 0.4, respectively. Two control supplements, high and low betaC, were prepared from commercial betaC beadlets. The high control supplement had an all-trans/9-cis ratio of 19.0, whereas 9-cis betaC was not detected in the low supplement. On d 27, serum and tissues were obtained for HPLC analysis of betaC and its isomers. Analysis of livers showed that all-trans betaC was the primary isomer present, but 9-cis and other isomers were also detected in all groups. The hepatic all-trans/9-cis ratios were 5.9, 4.9, 2.5, 1.4, 52.2 and47.5, respectively, for the groups listed above. Lower amounts of all-trans and 9-cis betaC were found in kidneys compared with the liver, but ratios of all-trans/9-cis were not different among groups. Only trace amounts of 9-cis betaC were found in serum. These results demonstrate that the algae D. bardawil and D. salina provide a bioavailable source of betaC isomers, but, as in humans, absorption of 9-cis betaC is poor and any 9-cis betaC absorbed is apparently cleared by the liver. 相似文献
122.
JW Finley PE Johnson PG Reeves RA Vanderpool M Briske-Anderson 《Canadian Metallurgical Quarterly》1994,42(2):81-96
Biliary/pancreatic (B/P) secretions are a major component of endogenous secretions, and endogenously secreted Zn is a primary means of Zn homeostasis. This study examined whether B/P fluid alters the absorption/reabsorption of Zn and, in doing so, whether this contributes to homeostatic control of Zn. Animal experiments utilized rats fed 10 or 300 micrograms Zn/kg diet. An open-ended gut perfusion study in which 65Zn-labeled B/P fluid or 67Zn-labeled and digested diet found significantly decreased Zn absorption from B/P fluid. Although Zn absorption from both sources was less in animals fed diets higher in Zn, there was no interaction of treatment and diet. Further studies utilizing cultured human colon carcinoma cells (CACO-2) as in vitro models of gut enterocytes found that the presence of B/P fluid significantly decreased Zn retention and/or transport and resulted in a redistribution of cellular Zn after 1200 min of incubation. These studies show that a substance in B/P fluid can decrease the absorption of Zn and also suggest that dietary Zn and Zn associated with B/P secretions are absorbed from distinct pools. However, the lack of an interactive effect with diet, and the amount of time required to see differences in CACO-2 cells, suggest that differences in absorption are not a major contributor to Zn homeostasis. 相似文献
123.
124.
Terry Carolyn J.; Blake Colin C. F. 《Protein engineering, design & selection : PEDS》1992,5(6):505-510
The structure of cleaved thyroxine-binding globulin (TBG) hasbeen modelled on the crystal structure of cleaved 1-antitrypsin(a member of the serine proteinase inhibitor, serpin, superfamily)based on the high sequence homology exhibited by the two proteins.Particular attention was paid to the identification and modelledcharacteristics of the thyroxine binding site. The primary aimof the study was to compare the site qualitatively with thecrystallographically determined binding site of transthyretin,the other major transporter of thyroxine, in an attempt to explainthe higher binding affinity of the site compared with the knownthyrox ine binding site in transthyretin (1010 versus 108 M1).The proposed binding site shares some similar characteristicswith the transthyretin binding site but also includes a clusterof aromatic residues which are entirely absent in transthyretin.It is proposed that this might account for the substantial differencein binding affinities. 相似文献
125.
Statistical detection of mass malware has been shown to be highly successful. However, this type of malware is less interesting to cyber security officers of larger organizations, who are more concerned with detecting malware indicative of a targeted attack. Here we investigate the potential of statistically based approaches to detect such malware using a malware family associated with a large number of targeted network intrusions. Our approach is complementary to the bulk of statistical based malware classifiers, which are typically based on measures of overall similarity between executable files. One problem with this approach is that a malicious executable that shares some, but limited, functionality with known malware is likely to be misclassified as benign. Here a new approach to malware classification is introduced that classifies programs based on their similarity with known malware subroutines. It is illustrated that malware and benign programs can share a substantial amount of code, implying that classification should be based on malicious subroutines that occur infrequently, or not at all in benign programs. Various approaches to accomplishing this task are investigated, and a particularly simple approach appears the most effective. This approach simply computes the fraction of subroutines of a program that are similar to malware subroutines whose likes have not been found in a larger benign set. If this fraction exceeds around 1.5 %, the corresponding program can be classified as malicious at a 1 in 1000 false alarm rate. It is further shown that combining a local and overall similarity based approach can lead to considerably better prediction due to the relatively low correlation of their predictions. 相似文献
126.
127.
We have employed a Sagnac interferometer to measure small optical phase shifts induced by the Fresnel drag effect on moving media. The system detects volumetric flow rates of water as slow as 43 μL/min while maintaining a small pressure drop across the measuring pipe. Velocity profiles and turbulence measurements of flowing water are also demonstrated and discussed. 相似文献
128.
CM Otto MC Mickel JW Kennedy EL Alderman TM Bashore PC Block JA Brinker D Diver J Ferguson DR Holmes 《Canadian Metallurgical Quarterly》1994,89(2):642-650
BACKGROUND: To identify predictors of long-term outcome after balloon aortic valvuloplasty, we analyzed data on 674 adults (mean age, 78 +/- 9 years; 56% were women) undergoing this procedure at 24 clinical centers who had a mean initial increase in aortic valve area of 0.3 cm2. METHODS AND RESULTS: Baseline data included clinical, echocardiographic, and catheterization variables. Follow-up data included mortality, cause of death, rehospitalization, 6-month echocardiography, and functional status. Kaplan-Meier curves and log-rank tests were used to evaluate survival in subgroups. Multivariate Cox regression models were used to identify independent predictors of survival. Overall survival was 55% at 1 year, 35% at 2 years, and 23% at 3 years, with the majority of deaths (70%) classified as cardiac by an independent review committee. Rehospitalization was common (64%), although 61% of survivors at 2 years reported improved symptoms. Echocardiography at 6 months (n = 115) showed restenosis from the postprocedural valve area of 0.78 +/- 0.31 cm2 to 0.65 +/- 0.25 cm2 (P < .0001). With stepwise multivariate analysis, sequentially adding clinical, echocardiographic, and catheterization variables, the overall model identified independent predictors of survival as baseline functional status, baseline cardiac output, renal function, cachexia, female gender, left ventricular systolic function, and mitral regurgitation. Baseline and postprocedural variables were examined to identify which subgroup of patients has the best outcome after aortic valvuloplasty. A "lower-risk" subgroup (28% of the study population), defined by normal left ventricular systolic function and mild clinical functional limitation, had a 3-year survival of 36% compared with 17% in the remainder of the study group. CONCLUSIONS: Long-term survival after balloon aortic valvuloplasty is poor with 1- and 3-year survival rates of 55% and 23%, respectively. Although survivors report fewer symptoms, early restenosis and recurrent hospitalization are common. 相似文献
129.
130.
P. B. Robinson J. M. Boulton-Stone J. R. Blake 《Journal of Engineering Mathematics》1995,29(5):393-412
The boundary integral method is applied to model the initial motion of two-dimensional or cylindrical deformable gas bubbles in an inviscid, incompressible fluid. Following the success of recent boundary integral studies to predict the qualitative behaviour of a single gas bubble, this numerical study is extended to consider the interaction of several bubbles. Surface tension, relative initial position and volume are all found to be important factors affecting the bubble interaction, jet formation, trapping of fluid between bubbles and bubble shedding. As well as computing the evolution of the bubble surfaces, consideration of the pressure fields and resulting instantaneous streamlines is given. 相似文献